The invention belongs to the technical field of flower and tree plant cultivation, and particularly belongs to a novel root system inducing method adopted before potting of hydroponic flowers and trees. The method comprises the following steps that a, under the condition that the air temperature is not lower than 15 DEG C, field cultivation seedlings or hole pot seedlings or cuttage bars of indoor cultivated flowers and trees are selected and washed, and rotten roots and leaves are removed; b, seedling plants or the cuttage bars are soaked in a 1,000-1,5000-fold antibacterial agent solution for 15-30 seconds, the seedling plants or the cuttage bars are taken out to be aired, and then the roots of the seedling plants or the cuttage bars are soaked in clear water for 10-20 hours; c, compound inducer inducing is carried out, a water solution is prepared from 0.2-0.5 mg / L 6-benayl aminopurine, 0.1-0.2 mg / L naphthylacetic acid, 0.02 mg / L gibberellins and 0.3 mg / L kinetin for soaking the roots or stem basal parts or cuttage bar basal parts for 30-60 minutes, and the seedling plants or the cuttage bars are taken out to be aired; d, shading culture is carried out, root system differentiation is promoted, culture is carried out in a proper shading greenhouse, hydroponic roots can grow out generally within 5-15 days, and meanwhile absorption roots and aerial roots are differentiated out.