45results about How to "High live bacteria" patented technology

The invention relates to lactobacillus reuteri and application thereof. Lactobacillus reuteri is lactobacillus reuteri LZ262 which is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms on 13 August, 2015 with the preservation number of CGMCCNo.11240. Lactobacillus reuteri LZ262 is from the intestinal tract of a healthy pig, can be colonized in a human body and an animal body, and has higher tolerance to gastric acid and bile salt; in addition, lactobacillus reuteri LZ262 has a better suppression effect on Escherichia coli, and has a higher survival rate after spray-drying, and higher fermentation density is achieved by improving a fermentation formula; the strain has a better effect of synergistic growth with other types of lactic acid bacteria, can be used for food and animal feed additives, and can also be widely applied to other food and biological products.

The invention relates to a probiotic solid beverage and a preparation method thereof. The probiotic solid beverage is prepared from, by weight, 25-30 parts of inulin, 5-10 parts of cranberry extractive and 1-5 parts of probiotics; the probiotics comprises bifidobacterium lactis, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus casei, lactobacillus reuteri, lactobacillus plantarumand bifidobacterium longum. The probiotic solid beverage can reduce the loss of the probiotics in the process of passing through the gastrointestinal tract, improve the survival rate, promote the colonization of the probiotics in the intestinal tract, has the functions of adjusting intestines and stomach, relieving constipation and diarrhea, adjusting body immunity and adjusting the blood glucoselevel, particularly can significantly improve gynecologic inflammation and female blotches, dark skin and dry hair caused by the constipation.

The invention discloses an astragalus membranaceus micro-ecologic preparation as well as a preparation method and an application thereof, and belongs to the technical field of feed additives and biological fermentation tradition Chinese medicines. The micro-ecologic preparation is prepared by fermenting astragalus membranaceus, a lactic acid bacteria seed solution and a solid culture medium, wherein the lactic acid bacteria seed solution is prepared by mixing and culturing enterococcus faecium and lactobacillus plantarum at a ratio of (2-8): (8-2); the number of viable organisms in the inoculated lactic acid bacteria seed solution, corresponding to every 25-75 g of astragalus membranaceus, is 25*109; compared with the traditional additive which directly mixes the lactic acid bacteria and the astragalus membranaceus, the astragalus membranaceus micro-ecologic preparation has the effect that the yield of the crude polysaccharide and the quantity of the lactic acid bacteria are improved after fermentation. According to the preparation method of the astragalus membranaceus micro-ecologic preparation, the synergistic effect of the lactic acid bacteria and the astragalus membranaceus is improved by selecting proper fermentation temperature and fermentation time. The astragalus membranaceus micro-ecologic preparation is used as a livestock feed additive.

The invention discloses a culture medium for effectively prolonging the survival time of lactic acid bacteria and a preparation method of the culture medium. The culture medium for effectively prolonging the survival time of the lactic acid bacteria is prepared by taking peptone, yeast extract powder, mannan oligosaccharide, skim milk powder, defatted soybean meal, magnesium sulfate heptahydrate, manganese sulfate heptahydrate, dried corn steep liquor powder, a compound additive, sodium dihydrogen phosphate, sodium citrate, citric acid, sodium acetate, Tween-80 and saccharomyces cerevisiae as raw materials; in addition, the compound additive is a mixture of sodium carboxymethylcellulose, sodium alginate, bone meal, royal jelly acid, guar gum and xanthan gum. According to the culture medium for effectively prolonging the survival time of the lactic acid bacteria, the functions of all the raw materials are mutually cooperative, and higher variable bacteria quantity of the lactic acid bacteria can be kept at normal temperature for a long time; in addition, the culture medium has the advantages of simplicity and convenience in operation, easily-obtained raw materials, low cost and high homogenization degree of a product; the lactic acid bacteria is enabled to be more stable in property and higher in biological activity and can be stored for a longer time.

The invention provides a lactobacillus preservative capable of increasing the survival rate of liquid lactobacilli at the room temperature and an application of the lactobacillus preservative. With adoption of a lactobacillus body and nutrient suspension technology and a sustained release technology as well as addition of cream, linoleic acid, lecithin, glycerol monostearate and a stabilizer to a medium and regulation of pH value, the storage period of the liquid lactobacilli with high live bacteria quantity is prolonged, the problem of rapid reduction of liquid lactobacilli with high live bacteria quantity due to reduction of the content of nutrients and storage at the normal temperature is solved, and the lactobacilli really play a role in preventing and treating diarrhea and regulating balance of the intestinal flora during use; the lactobacillus preservative as a green, safe and effective microecological agent is applied and popularized.

The invention discloses bacillus polymyxa DYr4.4 with broad spectrum antibacterial activity and a preparation method and application. The number is CCTCC M 2018023. The method comprises the steps thatA, a single bacterial colony of a bacterial strain is selected under the aseptic environment, inoculated to a culture solution and placed in a constant-temperature culture oscillator, the temperatureand rotation speed are adjusted, and oscillation culture is conducted; B, a fermentation solution is placed in each bottle, the bottles are placed in a large-amplitude constant-temperature freezing shaking table, and oscillation culture is conducted; C, the culture solution is prepared, wherein solutions are added to a tank in sequence, a tank body is sealed, the culture solution is heated and disinfected, an inoculation port is disinfected by igniting alcohol, 2 L of fermentation solution prepared in the last step is poured into the tank under the condition that the alcohol burns continuously, and the rotation speed of a stirrer is adjusted; D, the fermentation solution is filtered, an auxiliary material namely light calcium carbonate powder is added to the filtered fermentation solution, the mixed solution is placed in a centrifugal spray drying tower and dried, and dry powder is collected. The bacillus polymyxa DYr4.4 is applied to preparation of medicine for treating or preventingrust diseases of pear trees. Dry powder fermentation of the bacterial strain has the remarkable anti-disease effect on the rust diseases of the pear trees, the prevention effect reaches 67.09% or above, and leaf falling of the pear trees in advance is remarkably inhibited.

The invention discloses efficient bio-organic fertilizer which comprises straw, livestock excrement, leftovers, winery sewage, lees, bagasse, discarded traditional Chinese medicine, mildewed grain, magnesium nitrate, egg shells, waste paper, wheat bran, rape seed cake, bean pulp and strains. The efficient bio-organic fertilizer is free from toxicity, harmless, environmentally friendly and antibacterial, prevents diseases, and can improve and repair soil, increase organic matter of soil, improve the water and fertilizer preserving ability of the soil, increase the yield, prevent water from being polluted and protect environment; waste is used as a raw material, the problem of environmental pollution caused by industry, agriculture and life garbage is reduced, the waste is turned into wealth, nutritional ingredients of the organic fertilizer can be increased, crops grow well, and the yield and the nutritional ingredients of the crops are increased; multiple compound bacteria are fermented to form the fertilizer, the content of effective living bacteria is high, fermentation time is shortened obviously, working efficiency is improved, the multiple bacterial strains grow coordinately, and the shelf life can be prolonged to be 5 years.

The invention discloses a whey protein solid beverage containing N-acetylneuraminic acid. The beverage is prepared from sugar, concentrated whey protein powder, whole milk powder, N-acetylneuraminic acid, probiotics, prebiotics, ethylenediaminetetraacetic acid ferric sodium salt and casein phosphopeptides. According to the whey protein solid beverage, all raw materials are effectively combined, the immunity of all kinds of people including infants and young children can be effectively enhanced, the whey protein content is high, nutrition is rich, absorption is facilitated, and more sufficientnutrition and energy can be provided for drinkers.

The invention discloses a preparation method of a microbial fermentation medium. The method comprises steps that: (a) raw materials are pretreated, wherein green straws of crops are dried in the shade or dried by baking; and the dried straws are segmented, crushed, and sieved; (b) the straw powder is added to hot water with a temperature of 60 to 80 DEG C for lixiviating, wherein a mass ratio of straw powder to water is 1:50 or 1:10; the extract is settled and filtered; a carbon source is added to the clarified extract; the pH value of the extract is regulated to 7.0; and the extract is sterilized, such that a microbial fermentation medium or a concentrate thereof is obtained. According to the preparation method of the microbial fermentation medium, green straws of crops are adopted as a raw material, the cost is low, the preparation method is simple, the fermentation effect is good, the living microbe content is high, the raw materials are economic and environment-friendly, and the raw materials are sufficient and easy to obtain. No chemical pollution is brought in during the application of a prepared microbial manure product. The medium is especially suitable for the fermentation production of rhizobia, azotobacteria and phosphorus-solubilizing bacteria.

The invention relates to the technical field of pharmaceuticals and discloses a female probiotic composition and a preparation method and application thereof and a female care product. By the female probiotic composition, technical problems of low strain effective survival rate and poor colonization performance in the prior art can be solved, uncomfortable symptoms of female private parts can be effectively improved, vaginal pH value regulation is realized, and vaginal flora balance is recovered. In addition, the female probiotic composition has the advantage of effectiveness in improvement ofgynecological inflammations such as vaginitis, pelvic inflammation and cervicitis.

The invention discloses a fermentation medium for improving phosphate-dissolving ability of bacillus cereus and a fermentation method thereof. The medium comprises 25-35g / L saccharose, 0.8-1.2g / L NH4Cl, 0.3g of NaCl, 0.3g of KCl, 0.03g of FeSO4.7H2O, 0.03g of MnSO4.H2O, 0.3g of MgSO4.7H2O, 2.0g of KH2PO4, and 1,000mL of distilled water, and the pH is 7.0-7.5. The invention also provides a fermentation method of the fermentation medium for improving phosphate-dissolving ability of bacillus cereus. By adopting the fermentation medium and the fermentation method, the bacillus cereus is in the optimal growth state, the preparation efficiency and the phosphate-dissolving effect of the phosphate-solubilizing bacteria inoculant can be effectively improved, the production cost of the bactericide is reduced, and plant growth is facilitated.

The invention relates to a microorganism water purification agent and a preparation method thereof. The preparation method comprises following specific steps of: inoculating the agent onto a cane molasses medium, and fermenting for 24-120 hours at 25-35DEG C so as to cultivate the agent. The method for purifying the water quality of aquaculture water by the prepared microorganism water purification agent comprises the step of adding 15-18ml of the agent prepared by the method into one cube of aquaculture water. According to the method, the cane molasses medium is used for cultivating the agent, so that the amount of viable bacteria can be increased to the greatest extent, the amount of viable bacteria of bacillus subtilis, which plays a main role in purifying the water body, can be increased, and the purification effect of the aquaculture water is better; for the fermentation time, the amount of viable bacteria of the agent can be improved, furthermore, and the agent holds certain amount of sugar so as to provide nutrient for the bacteria, thus being beneficial to guaranteeing the quality; the agent prepared by the method can effectively solve the problem of damage and eutrophication of the quality of the aquaculture water, thus improving the quantity and quality of aquatic products.

The invention provides a straw micro-storage additive. The straw micro-storage additive is characterized by being prepared by fermenting lactic acid bacteria, brewers yeast, bacillus subtilis, actinomycetes, nitrogen-fixing bacteria, and a mould strain in a ratio of (75,300-10,000):(73,000-10,000):(600-500):(70-50):(15-10):1. A method for preparing the straw micro-storage additive comprises the following steps of: activating the strain, preparing seed liquid, sterilizing fermentation equipment and a culture medium, inoculating, designing fermentation parameters, collecting thallus, performingvacuum freeze drying, preparing a fermentation agent and the like. The straw micro-storage additive has the advantages of scientific proportioning, complete function, and high straw treatment performance. The scientific compatibility and mutual coordination of cellulose decomposing bacteria and beneficial bacteria in animal intestines can effectively improve nutrition structures of straws. After the straws in which the additive is added are fermented at the temperature of 37 DEG C for 15 days, the pH value can be reduced to 3.8, the content of crude protein is improved by about 8 percent, 15.05 percent of cellulose can be degraded, the water content is about 50 percent, the corruption rate is low, the color is yellowish, the texture is loose and soft, and the odor is mellow and strong; moreover, the preparation process is simple and practical.

The invention discloses a luminescent bacterium freeze-drying protective agent, freeze-dried powder and application of the freeze-dried powder in on-line monitoring of comprehensive toxicity of waterquality. Each L of the luminous bacterium freeze-drying protective agent is composed of the following components: 100-200g of skim milk, 20-120g of cane sugar, 10-60g of sodium chloride and the balance of water. The luminescent bacterium freeze-dried powder is prepared by adding a luminescent bacterium freeze-drying protective agent into a luminescent bacterium solution. The luminescent bacteriumfreeze-drying protective agent or the luminescent bacterium freeze-dried powder can be used as a detection preparation for on-line water quality toxicity monitoring. The resuscitated luminous bacterium freeze-dried powder is refrigerated in a strain storage cup at 4 DEG C, can be used for an online toxicity monitoring instrument for 15 days, can replace an imported freeze-drying protective agent,reduces the operation cost of reagent consumables of the online toxicity monitoring instrument, improves the continuous and stable operation of the online toxicity monitoring instrument, and plays a role in biotoxicity early warning for the safety of water quality.

The invention discloses a probiotic powdery agent. The probiotic powdery agent includes the following components in parts by mass: 5-10 parts of probiotic powder, 300-350 parts of a probiotic protection agent, and 20-30 parts of a sweetener, wherein the probiotic powder includes lactobacillus plantarum, bifidobacterium bifidum and lactobacillus casei, the total bacterial content of the probiotic powder is greater than 16 billion cfu / g, and the lactobacillus casei accounts for 55%-65% of a total weight of the probiotic powder; and the probiotic protection agent includes skimmed milk, inulin, trehalose, maltodextrin and cereal fiber powder, the skimmed milk accounts for 50%-60% of a total weight of the probiotic protection agent, and the cereal fiber powder accounts for 20%-30% of the totalweight of the probiotic protection agent. The probiotic powdery agent can effectively regulate intestinal flora, promote growth of probiotics in an intestine, and ensure the live bacteria amount of the probiotics in the intestine; the probiotic protection agent protects the probiotic powder, especially the cereal fibers cooperate with the skimmed milk, so that the loss of the probiotics in extremeenvironments such as stomach acid and bile can be reduced, and the live bacteria amount of the probiotics when the probiotics reach the intestine can be improved; and at the same time, the cereal fibers cooperate with the probiotics to realize intestinal conditioning.

The invention discloses a biological fermentation product for preventing and treating colitis and a preparation method thereof. The biological fermentation product has a certain preventing and treating effect to the colitis, and is high in stability. Compared with a control group, the viable bacteria quantity and polysaccharide content of fermented clostridium butyricum are greatly increased, thefact that dimocarpus longan extract liquid can promote the growth of the clostridium butyricum is demonstrated, the performance of a product containing a clostridium butyricum probiotics preparation is further improved, and the biological fermentation product plays an important role in the bioengineering fermentation field.

The invention relates a Lactobacillus acidophilus and an application thereof. The Lactobacillus acidophilus is Lactobacillus acidophilus FZU-LA1301X with the preservation number of CGMCC No.12453, and is preserved in China General Microbiological Culture Collection Center on May 16, 2016. The Lactobacillus acidophilus FZU-LA1301X can be colonized in weaned piglets, has strong tolerance to gastric acid, bile salt and intestinal juice, has a good live bacterium number in an improved fermentation medium, has a high survival rate after being spray-dried, and can be used in animal feed additives.

The invention discloses a preparation method of probiotic gel particles. The probiotic gel particles are prepared from beta-cyclodextrine, sodium alginate, konjac glucomannan, edible oil and fat, a probiotic culture solution, a probiotic protecting agent and water through the steps of sol solution preparation, probiotic culture solution preparation, probiotic powder coagulant preparation, probiotic mixed base material preparation, probiotic sol preparation and probiotic gel particle preparation. The probiotic gel particles can be added into various probiotic liquid beverages. The probiotic gelparticles prepared by the invention can keep the activity of probiotics in probiotic liquid beverages, and are particularly applicable to beverages with demand for long-term quality retention. Through specific probiotic culture and probiotic powder coagulant preparation, probiotic loss in extreme environments such as gastric acid and bile is effectively reduced, and the amount of viable bacteriareaching the intestinal tract is increased.

The invention discloses trichoderma harzianum and an an application thereof, the Latin name is Trichoderma harzianum, the strain number of the trichoderma harzianum is TR2125, and the preservation number of the trichoderma harzianum is CGMCC No.21430. The fermentation liquor obtained by fermenting the trichoderma hirsutum is used for preparing a microbial agent, and the obtained microbial agent has a good prevention and treatment effect on tomato damping-off; the fermentation liquor is not easy to cake during storage, is free of dust pollution during application and is convenient to apply; and the product has the advantages of good dispersion performance and high viable count, the spore content reaches 3*10 <8> CFU / g, and the chlamydospore content is still more than 10<8> CFU / g after the product is stored for 12 months.

The invention discloses a method of promoting propagation of candida tropicalis through sweep-frequency ultrasonic treatment on candida tropicalis in a mid-log phase and relates to the field of bioengineering. The method comprises inoculating a basic medium with a candida tropicalis strain as a raw material, carrying out strain activation, determining a growth curve, inoculating a seed medium with the activated candida tropicalis strain liquid, carrying out enlarge cultivation, carrying out moderate sweep-frequency ultrasonic treatment on the enlarge cultivation liquid under a certain condition, and culturing the treated enlarge cultivation liquid under appropriate conditions to obtain a candida tropicalis strain culture liquid product. The method can promote the proliferation of candida tropicalis, greatly increase the amount of viable bacteria and significantly reduce a cost of seed liquid fermentation production, has great economic and social benefits and has a good application prospect.

The present invention relates to a plant lactobacillus and its application. The plant lactobacillus is a plant lactobacillus (Lactobacillus plantarum) BJYL132, the preservation number is: CGMCC No.9646, and the preservation date is September 11, 2014. Unit: General Microbiology Center of China Committee for Culture Collection of Microorganisms. The Lactobacillus plantarum BJYL132 is derived from naturally fermented food, can colonize human bodies and animals, and is resistant to gastric acid and bile salts; at the same time, the bacterium has a good inhibitory effect on Escherichia coli and can survive at room temperature for 30 Day; this strain has a better synergistic growth effect with other types of lactic acid bacteria, and can be used in E. coli antibacterial liquid, aquaculture, and can also be widely used in other food and biological products fields.

The invention relates to a licorice microecological preparation fermented by Bacillus subtilis liquid, which is obtained by inoculating Bacillus subtilis seed liquid into licorice fermentation medium and culturing by shaking; the formula of the licorice fermentation medium is: licorice water extract 0.5-1.5mg / L, LB liquid medium 250mg / L, pH 7.4. The present invention utilizes Bacillus subtilis to ferment licorice in a liquid, so the production cost is low, and at the same time, it promotes the increase of the viable bacteria of Bacillus subtilis and the formation of spores, and after fermentation, it becomes a new type of microecological preparation, which is added to the drinking water of livestock and poultry to improve the digestibility of livestock and poultry, and the production performance and immunity.

The invention discloses a biological fermentation product for preventing and treating animal colitis and a preparation method thereof, which has a certain preventive effect on colitis, and the invention has high stability. compared to a significant increase. It shows that the longan extract can promote the growth of Clostridium butyricum, further improve the product performance of probiotic preparations containing Clostridium butyricum, and play an important role in the field of bioengineering fermentation.

The invention discloses a preparing method and application of high-density cultured nitrogen-fixing bacteria, and belongs to the technical field of bioengineering. The culture method comprises the steps that biochar serves as an adsorbent, nitrogen-fixing bacteria serve as an original strain, test tube expansion culture, liquid shake flask culture, seed tank expansion culture and liquid fermentation culture are carried out in sequence, and the average thallus density of the cultured nitrogen-fixing bacteria reaches 5*1013-1*1015 cfu / mL. Obtained fermentation liquid can be subjected to centrifugation and spray drying, and the quantity of viable bacteria in dried thallus powder reaches up to 1015-1017 cfu / g. The obtained nitrogen-fixing bacteria can serve as a live bacteria agent of bio-fertilizer or as a live bacteria agent of bio-fertilizer together with other strains.

The invention discloses a method for preparing a vector substrate of a plant growth promoting bacterium agent from green straws, which comprises the following steps of: (a) raw material processing; (b) raw material extracting; (c) filtration and neutralization; and (d) drying. In the method, the raw materials are sufficient and the price is low. Excessive soluble saccharides and proteins in the green straws are removed by adopting a hot water extracting or microwave irradiation extracting method, so that only a small amount of soluble carbon and nitrogen sources exists in green straw powder and more slightly soluble nutrient substances are decomposed and utilized by planted strains. The method has simple preparation process, good bacterium carrying effect and large viable bacteria quantity. The invention effectively solves the problems of the currently used bacterium agent vector. The method has high practicality and economy. The production and acquisition of the raw materials are environmental-friendly and non-polluted. The prepared bacterium agent product is convenient to use. The vector substrate can be degraded after being applied into the soil and the negative effects cannot be generated to the soil environment.

The invention provides a straw micro-storage additive. The straw micro-storage additive is characterized by being prepared by fermenting lactic acid bacteria, brewers yeast, bacillus subtilis, actinomycetes, nitrogen-fixing bacteria, and a mould strain in a ratio of (75,300-10,000):(73,000-10,000):(600-500):(70-50):(15-10):1. A method for preparing the straw micro-storage additive comprises the following steps of: activating the strain, preparing seed liquid, sterilizing fermentation equipment and a culture medium, inoculating, designing fermentation parameters, collecting thallus, performing vacuum freeze drying, preparing a fermentation agent and the like. The straw micro-storage additive has the advantages of scientific proportioning, complete function, and high straw treatment performance. The scientific compatibility and mutual coordination of cellulose decomposing bacteria and beneficial bacteria in animal intestines can effectively improve nutrition structures of straws. After the straws in which the additive is added are fermented at the temperature of 37 DEG C for 15 days, the pH value can be reduced to 3.8, the content of crude protein is improved by about 8 percent, 15.05 percent of cellulose can be degraded, the water content is about 50 percent, the corruption rate is low, the color is yellowish, the texture is loose and soft, and the odor is mellow and strong; moreover, the preparation process is simple and practical.