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Astragalus membranaceus micro-ecologic preparation as well as preparation method and application thereof

A technology of micro-ecological preparations and astragalus, applied in the fields of application, animal feed, animal feed, etc., can solve the problems of further increase in the increase rate of crude polysaccharides, increase of inorganic phosphorus pollution in livestock and poultry manure, and high cost of fermentation medium, to achieve increased Astragalus crude polysaccharide content and live lactic acid bacteria, the effect of improving animal production performance and increasing the content of crude polysaccharide and lactic acid bacteria

Active Publication Date: 2015-07-15
河南天昊鸿发生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zhu Xinshu disclosed in "Lactobacillus Breeding of Fermented Astragalus and Optimization of Fermentation Parameters" that the water extract of Astragalus fermented by Lactobacillus can improve the yield of Astragalus polysaccharides and the number of cells, and optimized the fermentation medium as corn flour 42.10 g / L, nitrogen source 7.45g / L, carrot powder 0.80g / L, KH 2 PO 4 -K 2 HPO 4 2.18g / L, meat extract 6.25g / L, the initial pH of the fermentation medium is 6.7, the fermentation time is 68h, and the fermentation temperature is 38°C. The fermentation medium uses lactic acid bacteria to ferment Astragalus membranaceus. Although the yield of crude polysaccharides has been increased, the increase rate of crude polysaccharides needs to be further improved, and the cost of the fermentation medium is high, and inorganic phosphorus is added, which increases the inorganic phosphorus pollution of livestock and poultry manure. The problem

Method used

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  • Astragalus membranaceus micro-ecologic preparation as well as preparation method and application thereof
  • Astragalus membranaceus micro-ecologic preparation as well as preparation method and application thereof
  • Astragalus membranaceus micro-ecologic preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:

[0030] 1) Raw material pretreatment:

[0031] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;

[0032] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust the pH to 6.8, and autoclave to prepare MRS medium. Enter the Enterococcus faecium and Lactobacillus plantarum into the MRS medium at a ratio of 5:5, and culture anaerobically at 37°C 24h, that is;

[0033] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;

[0034] 2) Ferment...

Embodiment 2

[0037] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:

[0038] 1) Raw material pretreatment:

[0039] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;

[0040] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust pH to 6.8, and autoclave to prepare MRS medium, Enterococcus faecium and Lactobacillus plantarum into MRS medium at a ratio of 5:5, anaerobic culture at 38°C 30h, that is;

[0041] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;

[0042]2) Fermented astragalus: mix 50 g of ...

Embodiment 3

[0045] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:

[0046] 1) Raw material pretreatment:

[0047] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;

[0048] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust the pH to 6.8, and autoclave to prepare MRS medium. Enter the Enterococcus faecium and Lactobacillus plantarum into the MRS medium at a ratio of 5:5, and cultivate anaerobically at 39°C 36h, that is;

[0049] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;

[0050] 2) Ferme...

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Abstract

The invention discloses an astragalus membranaceus micro-ecologic preparation as well as a preparation method and an application thereof, and belongs to the technical field of feed additives and biological fermentation tradition Chinese medicines. The micro-ecologic preparation is prepared by fermenting astragalus membranaceus, a lactic acid bacteria seed solution and a solid culture medium, wherein the lactic acid bacteria seed solution is prepared by mixing and culturing enterococcus faecium and lactobacillus plantarum at a ratio of (2-8): (8-2); the number of viable organisms in the inoculated lactic acid bacteria seed solution, corresponding to every 25-75 g of astragalus membranaceus, is 25*109; compared with the traditional additive which directly mixes the lactic acid bacteria and the astragalus membranaceus, the astragalus membranaceus micro-ecologic preparation has the effect that the yield of the crude polysaccharide and the quantity of the lactic acid bacteria are improved after fermentation. According to the preparation method of the astragalus membranaceus micro-ecologic preparation, the synergistic effect of the lactic acid bacteria and the astragalus membranaceus is improved by selecting proper fermentation temperature and fermentation time. The astragalus membranaceus micro-ecologic preparation is used as a livestock feed additive.

Description

technical field [0001] The invention belongs to the technical field of feed additives and bio-fermentation traditional Chinese medicines, and specifically relates to a microecological preparation, a preparation method and an application. Background technique [0002] With the abuse of antibiotics, hormones, etc. Additives will have more and more important applications in animal husbandry production. Microecological preparations are probiotics, prebiotics, and combinations of probiotics and prebiotics. The important sources of probiotics and prebiotics are lactic acid bacteria and plant polysaccharides respectively. However, traditional microecological preparations simply combine lactic acid bacteria and plant polysaccharides. The huge potential of microbial fermentation has not been brought into play. Studies have shown that microbial fermentation of traditional Chinese medicine has outstanding advantages such as increasing the content of active ingredients in traditional C...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14
Inventor 乔宏兴边传周王永芬史洪涛赵圣振李华玮姜亚乐张菡
Owner 河南天昊鸿发生物技术有限公司
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