Astragalus membranaceus micro-ecologic preparation as well as preparation method and application thereof
A technology of micro-ecological preparations and astragalus, applied in the fields of application, animal feed, animal feed, etc., can solve the problems of further increase in the increase rate of crude polysaccharides, increase of inorganic phosphorus pollution in livestock and poultry manure, and high cost of fermentation medium, to achieve increased Astragalus crude polysaccharide content and live lactic acid bacteria, the effect of improving animal production performance and increasing the content of crude polysaccharide and lactic acid bacteria
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Embodiment 1
[0029] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:
[0030] 1) Raw material pretreatment:
[0031] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;
[0032] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust the pH to 6.8, and autoclave to prepare MRS medium. Enter the Enterococcus faecium and Lactobacillus plantarum into the MRS medium at a ratio of 5:5, and culture anaerobically at 37°C 24h, that is;
[0033] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;
[0034] 2) Ferment...
Embodiment 2
[0037] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:
[0038] 1) Raw material pretreatment:
[0039] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;
[0040] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust pH to 6.8, and autoclave to prepare MRS medium, Enterococcus faecium and Lactobacillus plantarum into MRS medium at a ratio of 5:5, anaerobic culture at 38°C 30h, that is;
[0041] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;
[0042]2) Fermented astragalus: mix 50 g of ...
Embodiment 3
[0045] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:
[0046] 1) Raw material pretreatment:
[0047] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;
[0048] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust the pH to 6.8, and autoclave to prepare MRS medium. Enter the Enterococcus faecium and Lactobacillus plantarum into the MRS medium at a ratio of 5:5, and cultivate anaerobically at 39°C 36h, that is;
[0049] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;
[0050] 2) Ferme...
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