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A kind of method for in vitro symbiosis culture of chestnut and fungus

A symbiotic culture and co-cultivation technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of low mycorrhizal formation efficiency, cumbersome cultivation steps, and long cycle, so as to improve the fungal infection rate and increase Interaction effect, effect of eliminating interference

Active Publication Date: 2021-10-01
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The object of the present invention is to provide a method for in vitro symbiosis culture of chestnut and fungus, to solve the technical problems of low mycorrhizal formation efficiency, cumbersome culture steps, long cycle, root damage, etc. existing in the prior art

Method used

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  • A kind of method for in vitro symbiosis culture of chestnut and fungus
  • A kind of method for in vitro symbiosis culture of chestnut and fungus
  • A kind of method for in vitro symbiosis culture of chestnut and fungus

Examples

Experimental program
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Effect test

Embodiment 1

[0064] The present embodiment provides a kind of method that Chinese chestnut and puffball scleroderma in vitro symbiosis culture comprise the steps:

[0065] (1) Pour the prepared fungal culture medium into a 13cm × 13cm petri dish, and spread a sterile glass fiber membrane (Sigma company, specification: Z377600-1PAK) half the size of the culture medium after the culture medium is completely solidified, Cover the entire dish with two sheets of sterile glass fiber membrane.

[0066] Wherein, the above-mentioned fungal medium is P20 medium, and the components are: Di-NH 4 -tartrat, 0.5g L -1 ;KH 2 PO 4 1g·L -1 ;MgSO 4 ·7H 2 O 0.5g·L -1 ; Glucose 1g·L -1 ; Trace element mother solution (1000×) 1mL·L -1 ; Ammonium sulfate hydrochloride mother liquor (1000×) 1mL L -1 ;MES 1g·L -1 ; Agar is 15g L -1 ; Use 0.25N KOH to adjust the pH to 5.8, and sterilize with damp heat at 121°C for 21 minutes.

[0067] (2) Inoculate the purified Puffball scleroderma on the petri dish in...

Embodiment 2

[0077] This example refers to the cultivation method of Example 1, the only difference is that in step (2), the fungus block is cultured in the dark at 25°C for 15 days to obtain pure hyphae grown on the glass fiber membrane without culture medium .

Embodiment 3

[0079] This example refers to the cultivation method of Example 1, the only difference is that in step (3), the hyphae sheet was cultured in the dark at 25°C for 5 days to obtain pure bacteria grown on the glass fiber membrane without culture medium Silk.

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Abstract

The invention relates to the technical field of mycorrhizal culture, in particular to a method for in vitro symbiotic culture of chestnuts and fungi. The method for in vitro symbiosis culture of chestnut and fungus comprises the steps of: co-cultivating the root system of chestnut and the pure hyphae of fungus, during the co-cultivation, the root system of chestnut and the pure mycelium are isolated from the culture medium of co-cultivation by using the first semipermeable membrane , the pore size of the first semipermeable membrane is 0.5 μm-0.7 μm. The method for in vitro symbiotic culture of chestnut root system and puffball sclerosteae of the present invention adopts specific semi-permeable membrane to physically separate the culture medium from the chestnut root system and pure hyphae in the co-cultivation system, but can ensure that the culture medium can provide nutrients for Chestnut root system and pure mycelium will not affect the normal growth of chestnut root system and pure mycelium.

Description

technical field [0001] The invention relates to the technical field of mycorrhizal culture, in particular to a method for in vitro symbiotic culture of chestnuts and fungi. Background technique [0002] Ectomycorrhizal fungi are symbionts formed by mutualistic symbiosis between fungi and plant roots. Fungi that can form such a symbiotic relationship are called ectomycorrhizal fungi. [0003] The effect of ectomycorrhizal on the vegetative growth of the host is to expand the absorption area and scope of the host. The second is to improve the absorption of nutrients. Studies have shown that ectomycorrhizae can improve the absorption and utilization of nutrients by host plants, especially the absorption and utilization of P and N. The third is the growth-promoting effect of ectomycorrhizal fungi secreting phytohormones. Most ectomycorrhizal fungi can synthesize or secrete phytohormones and growth regulators to regulate the symbiotic relationship, and play multiple roles in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G18/10A01G18/20A01G18/40
Inventor 曹庆芹秦岭王溢洋张昊琳葛月阳李虎臣李光栋邢宇张卿房克凤
Owner BEIJING UNIV OF AGRI
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