A kind of astragalus probiotics and its preparation method and application
The technology of a microecological preparation and astragalus is applied in the application, animal feed, additional food elements, etc., and can solve the problems of high cost of fermentation medium, the increase rate of crude polysaccharide needs to be further improved, and the increase of inorganic phosphorus pollution in livestock and poultry manure, etc. Achieve the effect of increasing the content of crude polysaccharide and lactic acid bacteria, increasing the content of crude polysaccharide and lactic acid bacteria in Astragalus, and improving animal production performance
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Embodiment 1
[0029] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:
[0030] 1) Raw material pretreatment:
[0031] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;
[0032] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust pH to 6.8, and autoclave to prepare MRS medium, Enterococcus faecium and Lactobacillus plantarum into MRS medium at a ratio of 5:5, anaerobic culture at 37°C 24h, that is;
[0033] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;
[0034] 2) Fermented astragalus: After mixin...
Embodiment 2
[0037] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:
[0038] 1) Raw material pretreatment:
[0039] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;
[0040] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust the pH to 6.8, and autoclave to prepare the MRS medium, enter the Enterococcus faecium and Lactobacillus plantarum into the MRS medium at a ratio of 5:5, and culture anaerobically at 38°C 30h, that is;
[0041] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;
[0042]2) Ferm...
Embodiment 3
[0045] The probiotics of Astragalus membranaceus in this example is prepared by the following method, and the specific operation steps are:
[0046] 1) Raw material pretreatment:
[0047] A: crush astragalus, corn flour, bran, apple pomace and papaya pomace through a 40-mesh sieve;
[0048] B: Preparation of lactic acid bacteria seed solution: take 10g of casein peptone, 10g of beef extract, 8g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 1g of Tween-80, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate, Manganese sulfate 0.05g, calcium carbonate 10g, water 1000mL, adjust the pH to 6.8, and autoclave to prepare MRS medium. Enter the Enterococcus faecium and Lactobacillus plantarum into the MRS medium at a ratio of 5:5, and cultivate anaerobically at 39°C 36h, that is;
[0049] C: get corn flour, bran, apple pomace and papaya pomace and mix according to equal mass ratio to make solid culture medium;
[0050] 2) Ferme...
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