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Fermentation medium for improving phosphate-dissolving ability of bacillus cereus and fermentation method thereof

A technology of Bacillus cereus and fermentation medium, which is applied in the field of fermentation medium to improve the phosphorus-solubilizing ability of Bacillus cereus, and can solve the problems of low biomass, high cost of medium, and unsuitability for fermentation and cultivation of Bacillus cereus , to achieve the effects of promoting plant growth, shortening the fermentation cycle, and reducing the production cost of bacterial agents

Inactive Publication Date: 2014-05-14
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to solve the problems of high cost, low biomass after fermentation and unsuitability for fermentation and cultivation of Bacillus cereus in the existing medium for cultivating phosphorus-solubilizing bacteria, and to provide a fermentation method for improving the phosphorus-dissolving ability of Bacillus cereus. Culture medium and fermentation method

Method used

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  • Fermentation medium for improving phosphate-dissolving ability of bacillus cereus and fermentation method thereof
  • Fermentation medium for improving phosphate-dissolving ability of bacillus cereus and fermentation method thereof

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Experimental program
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preparation Embodiment 1

[0036] Sucrose 10g / L, NH 4 Cl0.5g / L, NaCl0.3g, KCl0.3g, FeSO 4 ·7H 2 O0.03g, MnSO 4 ·H1 2 O0.03g, MgSO 4 ·7H 2 O0.3g, KH 2 PO4 2.0g, distilled water 1000mL, pH7.0~7.5.

preparation Embodiment 2

[0038] Sucrose 10g / L, NH 4 Cl1.0g / L, NaCl0.3g, KCl0.3g, FeSO 4 ·7H 2 O0.03g, MnSO 4 ·H 2 O0.03g, MgSO 4 ·7H 2 O0.3g, KH 2 PO4 2.0g, distilled water 1000mL, pH7.0~7.5.

preparation Embodiment 3

[0040] Sucrose 10g / L, NH 4 Cl1.5g / L, NaCl0.3g, KCl0.3g, FeSO 4 ·7H 2 O0.03g, MnSO 4 ·H 2 O0.03g, MgSO 4 ·7H 2 O0.3g, KH 2 PO 4 2.0g, distilled water 1000mL, pH7.0~7.5.

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Abstract

The invention discloses a fermentation medium for improving phosphate-dissolving ability of bacillus cereus and a fermentation method thereof. The medium comprises 25-35g / L saccharose, 0.8-1.2g / L NH4Cl, 0.3g of NaCl, 0.3g of KCl, 0.03g of FeSO4.7H2O, 0.03g of MnSO4.H2O, 0.3g of MgSO4.7H2O, 2.0g of KH2PO4, and 1,000mL of distilled water, and the pH is 7.0-7.5. The invention also provides a fermentation method of the fermentation medium for improving phosphate-dissolving ability of bacillus cereus. By adopting the fermentation medium and the fermentation method, the bacillus cereus is in the optimal growth state, the preparation efficiency and the phosphate-dissolving effect of the phosphate-solubilizing bacteria inoculant can be effectively improved, the production cost of the bactericide is reduced, and plant growth is facilitated.

Description

technical field [0001] The invention belongs to the technical field of exploring the phosphorus-dissolving ability of bacillus cereus, and relates to a fermentation medium and a fermentation method for improving the phosphorus-dissolving ability of bacillus cereus. Background technique [0002] Phosphorus is an essential nutrient element for plant growth and development. Phosphorus deficiency can lead to a significant decrease in crop yields. Although the total phosphorus content in the soil is relatively high, it mostly exists in an insoluble state and is difficult to be absorbed and utilized by plants. In order to obtain high yield, a large amount of phosphorus fertilizers are repeatedly applied in production, about 70% of which are converted into insoluble compounds such as Ca-P, Fe-P and Al-P and stored in the soil, which not only depletes the limited phosphorus resources, but also causes Environmental pollution will also affect food safety and human health. [0003] M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/085
Inventor 余旋刘旭
Owner NORTHWEST A & F UNIV
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