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Method of promoting propagation of candida tropicalis through sweep-frequency ultrasonic treatment on candida tropicalis in mid-log phase

A technology of Candida tropicalis and frequency-sweeping ultrasound, applied in the field of bioengineering, can solve problems such as microbial cell stimulation, achieve the effects of promoting proliferation, increasing the amount of viable bacteria, and reducing costs

Inactive Publication Date: 2017-03-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the slight differences in the structure of the same microorganism during the growth and reproduction process, it is difficult to effectively stimulate all microbial cells with fixed-frequency ultrasonic waves

Method used

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  • Method of promoting propagation of candida tropicalis through sweep-frequency ultrasonic treatment on candida tropicalis in mid-log phase
  • Method of promoting propagation of candida tropicalis through sweep-frequency ultrasonic treatment on candida tropicalis in mid-log phase

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Candida tropicalis ( Candida tropicalis , CICC No.: 1254) The freeze-dried strains were revived in tubes according to CICC requirements, cultured statically in wort medium in a biochemical incubator at 28°C for 2 days and transferred for 3 generations. The growth curve of the activated Candida tropicalis was determined. The method for determining the growth curve was: take 2 rings of bacteria to 100 mL of YPD medium, and culture it in a shaker for 24 h. Put the above cultured bacterial solution into another 16 flasks containing 50 mL of YPD medium at a ratio of 10%, and label them as 1-16. Shake culture at 28°C and 160r / min, and every 1 hour for the first 4 hours. Take out a bottle of culture solution every 2 h, and then take out a bottle of culture solution every 2 hours, and take out 1 mL of bacterial solution, add 9 mL of distilled water for dilution, use YPD medium as a blank, and compare turbidity at 620 nm. Take the culture time as the abscissa and the OD valu...

Embodiment 2

[0033] Candida tropicalis ( Candida tropicalis , CICC No.: 1254) The freeze-dried strains were revived in tubes according to CICC requirements, cultured statically in wort medium in a biochemical incubator at 28°C for 2 days and transferred for 3 generations. The growth curve of activated C. tropicalis was determined according to the method and procedure described in Example 1.

[0034] Pick one ring of activated cultured cells into a 100 mL sterilized Erlenmeyer flask filled with YPD medium, and place them in a shaker for expanded culture at a temperature of 28 °C and a rotational speed of 160 r / min.

[0035] According to the measurement results of the growth curve, ultrasonic treatment was performed according to the steps described in Example 1 in the mid-log phase of the growth of Candida tropicalis in the expanded culture medium (cultured for 12 hours). The main conditions are ultrasonic treatment with a frequency of 28 kHz, frequency sweep range of ±0.5 kHz, ultrasoni...

Embodiment 3

[0038] Candida tropicalis ( Candida tropicalis , CICC No.: 1254) The freeze-dried strains were revived in tubes according to CICC requirements, cultured statically in wort medium in a biochemical incubator at 28°C for 2 days and transferred for 3 generations. The growth curve of activated C. tropicalis was determined according to the method and procedure described in Example 1.

[0039] Pick one ring of activated cultured cells into a 100 mL sterilized Erlenmeyer flask filled with YPD medium, and place them in a shaker for expanded culture at a temperature of 28 °C and a rotational speed of 160 r / min.

[0040] According to the measurement results of the growth curve, ultrasonic treatment was performed according to the steps described in Example 1 in the mid-log phase of the growth of Candida tropicalis in the expanded culture medium (cultured for 11 h). The main conditions are ultrasonic treatment with a frequency of 33 kHz, frequency sweep range of ±1.2 kHz, ultrasonic po...

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Abstract

The invention discloses a method of promoting propagation of candida tropicalis through sweep-frequency ultrasonic treatment on candida tropicalis in a mid-log phase and relates to the field of bioengineering. The method comprises inoculating a basic medium with a candida tropicalis strain as a raw material, carrying out strain activation, determining a growth curve, inoculating a seed medium with the activated candida tropicalis strain liquid, carrying out enlarge cultivation, carrying out moderate sweep-frequency ultrasonic treatment on the enlarge cultivation liquid under a certain condition, and culturing the treated enlarge cultivation liquid under appropriate conditions to obtain a candida tropicalis strain culture liquid product. The method can promote the proliferation of candida tropicalis, greatly increase the amount of viable bacteria and significantly reduce a cost of seed liquid fermentation production, has great economic and social benefits and has a good application prospect.

Description

[0001] Technical field: [0002] The invention relates to the field of bioengineering, in particular to a microbial seed liquid culture technology for industrial fermentation, in particular to a technical method for treating Candida tropicalis in the mid-logarithmic growth stage with low-intensity pulse-sweeping ultrasonic radiation, thereby greatly increasing the number of growing cells. [0003] Background technique: [0004] Candida tropicalis ( Candida tropicalis ) can efficiently utilize various sugars in agricultural and sideline product wastes and convert them into proteins or polypeptides, so it is widely used in solid-state fermentation production such as fermented feed and fermented food. In recent years, the traditional microbial solid-state fermentation technology has attracted widespread attention. This method is low in cost, water-saving, energy-saving, and less emission, which is in line with the current green, energy-saving, and high-efficiency development direc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12R1/74
CPCC12N13/00
Inventor 何荣海熊锋侯福荣张韦唯邢欢马海乐
Owner JIANGSU UNIV
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