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Straw micro-stage additive and preparation method thereof
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An additive and straw technology, applied in the field of microorganisms, can solve problems such as traffic accidents that affect air quality, affect crop growth, reduce soil fertility, etc., achieve the effects of improving the internal and external environment of animals, solving the imbalance of organism flora, and having a wide range of uses
Active Publication Date: 2013-03-20
曾蜀斌
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[0005] The annual supply gap of pasture feed in my country is 280 million tons, and there are 600 million tons of straw resources urgently waiting to be treated every year. At present, the bad disposal of a large amount of straw, such as incineration, seriously affects the air quality and causes a large number of traffic accidents; such as direct landfill In the field, it will destroy the soil structure after a long time, reduce soil fertility and affect the growth of crops
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[0046] 1. Materials:
[0047]SW-CJ-1CU ultra-clean workbench, domestic; YXQ-LS-50SI vertical pressure steam sterilizer, domestic; FD-1A-50 freeze dryer, domestic; BIOF-6050A50L mechanical stirring stainless steel intelligent fermentation tank , domestic; TGL low-temperature refrigerated centrifuge, domestic; L2800 microscope, domestic; UVD-3500 ultraviolet spectrophotometer, American Labomed; 3000B electronic balance, American; strains: Saccharomyces cerevisiae, Bacillus subtilis, lactic acid bacteria, Cytobacteria, nitrogen-fixing bacteria, and molds are preserved in the Pharmaceutical Laboratory of the School of Pharmacy and Bioengineering, Chongqing University of Technology.
[0048] 2. Method:
[0049] 2.1 Strain activation:
[0050] Put the preserved brewer's yeast, lactic acid bacteria, Bacillus subtilis, actinomycetes, nitrogen-fixing bacteria, and mold strains into sterilized 1% sugar water to activate for 2 hours, and then transfer them into sterilized bean sprouts ...
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Abstract
The invention provides a straw micro-storage additive. The straw micro-storage additive is characterized by being prepared by fermenting lactic acid bacteria, brewers yeast, bacillus subtilis, actinomycetes, nitrogen-fixing bacteria, and a mould strain in a ratio of (75,300-10,000):(73,000-10,000):(600-500):(70-50):(15-10):1. A method for preparing the straw micro-storage additive comprises the following steps of: activating the strain, preparing seed liquid, sterilizing fermentation equipment and a culture medium, inoculating, designing fermentation parameters, collecting thallus, performing vacuum freeze drying, preparing a fermentation agent and the like. The straw micro-storage additive has the advantages of scientific proportioning, complete function, and high straw treatment performance. The scientific compatibility and mutual coordination of cellulose decomposing bacteria and beneficial bacteria in animal intestines can effectively improve nutrition structures of straws. After the straws in which the additive is added are fermented at the temperature of 37 DEG C for 15 days, the pH value can be reduced to 3.8, the content of crude protein is improved by about 8 percent, 15.05 percent of cellulose can be degraded, the water content is about 50 percent, the corruption rate is low, the color is yellowish, the texture is loose and soft, and the odor is mellow and strong; moreover, the preparation process is simple and practical.
Description
technical field [0001] The invention relates to micro-storage additives and belongs to the technical field of microorganisms. Background technique [0002] Straw micro-storage feed is a new straw processing technology that has developed rapidly in recent years. It uses microorganisms to ferment in straw to make straw into roughage with sour, fragrant and wine flavors. It is an energy-saving, environmentally friendly and efficient agricultural product. Farm products. Micro-storage feed has been widely used because of its low cost, high benefit, good palatability, high feed intake, wide source of feed, and not subject to seasonal restrictions. The national and local governments vigorously advocate the development of "saving society", "environmental protection industry" and "resource utilization industry", and have introduced a series of encouraging policies. The development and promotion of micro-storage feed products is in line with the national industrial policy orientatio...
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