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Biological fermentation product for preventing and treating colitis and preparation method thereof

A technology of biological fermentation and colitis, applied in the field of bioengineering, can solve problems such as short research time, and achieve the effect of high viable bacteria, increased polysaccharide content, and growth promotion

Active Publication Date: 2018-09-25
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research time of Clostridium butyricum is not long. Compared with other probiotic-related microecological preparations, the research of Clostridium butyricum probiotics is still in the initial stage
There are certain deficiencies in the microecological preparations containing Clostridium butyricum currently on the market

Method used

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  • Biological fermentation product for preventing and treating colitis and preparation method thereof
  • Biological fermentation product for preventing and treating colitis and preparation method thereof
  • Biological fermentation product for preventing and treating colitis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The fermentation process of embodiment 1 clostridium butyricum fermented longan

[0053] (1) Extraction method of longan extract

[0054] Take dried longan, mash and homogenate, mix water and longan pulp according to the liquid-to-solid ratio of 15ml:1g, and homogenize to obtain longan pulp slurry. Under the condition that the hydrolysis time was 187.0min, the enzyme hydrolysis extraction experiment was carried out on a shaker with a rotation speed of 120r / min. After the extraction, the enzyme was inactivated in a water bath at 80°C for 10 minutes. Filter through eight layers of gauze, discard the residue, and obtain the supernatant. The supernatant was concentrated under reduced pressure to 40% of the original volume to obtain the longan extract.

[0055] (2) Standard curve creation

[0056]Accurately weigh 100mg of dried glucose, dissolve it with deionized water and set the volume in a 100mL volumetric flask to obtain a 1mg / mL glucose standard solution. Accuratel...

Embodiment 2

[0069] The fermentation process of embodiment 2 clostridium butyricum fermentation longan

[0070] (1) Extraction method of longan extract

[0071] Take dried longan, mash and homogenate, mix water and longan pulp according to the liquid-to-material ratio of 20ml:1g, and homogenize to obtain longan pulp slurry, the amount of cellulase added is 2% w / v, the enzymolysis temperature is 60.0°C, and the enzyme Under the condition that the hydrolysis time was 210min, the enzyme hydrolysis extraction experiment was carried out on a shaker with a rotational speed of 120r / min. After the extraction, the enzyme was inactivated in a water bath at 80°C for 10 minutes, filtered through eight layers of gauze, and the residue was discarded to obtain the supernatant. The supernatant was concentrated under reduced pressure to 30% of the original volume to obtain a longan extract.

[0072] (2) Standard curve creation

[0073] Accurately weigh 100mg of dried glucose, dissolve it with deionized ...

Embodiment 3

[0085] The fermentation process of embodiment 3 clostridium butyricum fermented longan

[0086] (1) Extraction method of longan extract

[0087] Take dried longan, mash and homogenate, mix water and longan pulp according to the liquid-to-material ratio of 16ml:1g, and homogenize to obtain longan pulp slurry, the amount of cellulase added is 1.5% w / v, the enzymolysis temperature is 45.0°C, and the enzyme Under the condition of the hydrolysis time of 180min, the enzymolysis extraction experiment was carried out on a shaker with a rotation speed of 120r / min. After the extraction, the enzyme was inactivated in a water bath at 80°C for 10 minutes, filtered through eight layers of gauze, and the residue was discarded to obtain the supernatant. The supernatant was concentrated under reduced pressure to 30% of the original volume to obtain a longan extract.

[0088] (2) Standard curve creation

[0089] Accurately weigh 100mg of dried glucose, dissolve it with deionized water and se...

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Abstract

The invention discloses a biological fermentation product for preventing and treating colitis and a preparation method thereof. The biological fermentation product has a certain preventing and treating effect to the colitis, and is high in stability. Compared with a control group, the viable bacteria quantity and polysaccharide content of fermented clostridium butyricum are greatly increased, thefact that dimocarpus longan extract liquid can promote the growth of the clostridium butyricum is demonstrated, the performance of a product containing a clostridium butyricum probiotics preparation is further improved, and the biological fermentation product plays an important role in the bioengineering fermentation field.

Description

technical field [0001] The invention relates to a biological fermentation product for preventing and treating animal colitis and a preparation method thereof, belonging to the field of bioengineering. Background technique [0002] As a feed additive, Clostridium butyricum can promote growth and improve feed conversion rate, and it is a strain with great utilization value and development prospect. However, the research time of Clostridium butyricum is not long. Compared with other probiotic-related microecological preparations, the research of Clostridium butyricum probiotics is still in its initial stage. There are certain deficiencies in the probiotics containing Clostridium butyricum currently on the market. And liquid fermentation is the key step of producing Clostridium butyricum technology, wherein the character of fermentation medium, fermentation condition (temperature, pH and inoculum size etc.) all can directly influence the level of liquid fermentation of Clostrid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A61P1/00A61P29/00A61P1/04
CPCA61K36/77A61K2236/19A61K2236/51A61P1/00A61P1/04A61P29/00
Inventor 徐志宏梁伟杰彭新宇袁明贵向蓉黄婷涂杜高彪
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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