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Lactobacillus acidophilus and application thereof

A technology of Lactobacillus acidophilus and bacterial agent, which is applied to Lactobacillus acidophilus and its application field, and can solve the problems of poor temperature resistance of Lactobacillus acidophilus, rapid decline in activity, and difficulty in reaching the number of viable bacteria.

Active Publication Date: 2016-10-12
XIAMEN LONGLI IND & TRADE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the temperature resistance of Lactobacillus acidophilus is not good, the activity declines rapidly, and the number of viable bacteria in the product is difficult to reach the specified minimum limit of viable bacteria 10 6 cfu / g

Method used

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  • Lactobacillus acidophilus and application thereof
  • Lactobacillus acidophilus and application thereof
  • Lactobacillus acidophilus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Mutation acclimation of Lactobacillus acidophilus FZU-LA1301X

[0025] Centrifuge the MRS fermentation broth at 4000r / m for 15min, resuspend it in normal saline, and adjust the cell concentration to 10 8 cfu / mL, made into cell suspension. After the bacterial suspension is irradiated at the optimal mutagenesis time (lethal rate is 70%-80%), it is diluted to an appropriate multiple and spread on MRS solid medium, and cultured at 37°C for 24 hours to withstand digestion For the screening index, the best mutant strain Lactobacillus acidophilus FZU-LA1301Y was selected.

[0026] The mutant strains obtained by ultraviolet mutagenesis were inoculated in MRS liquid medium and cultured at 37°C to logarithmic growth phase, then transferred to 42°C environment for 24 hours, and then transferred to MRS liquid medium, and the survival rate was counted on the plate. Passage repeatedly at 42°C for 5 generations, so that the survival rate of 2-3 consecutive generations can reach more...

Embodiment 2

[0030] Experiment of Lactobacillus acidophilus FZU-LA1301X resistance to digestion

[0031] 1. Relevant formula

[0032] (4) Preparation of artificial gastric juice

[0033] Adjust the pH value of MRS liquid medium to 3.0, 3.5, 4.0, 4.5 with 1mol / L HCl, sterilize at 121°C for 20min, add pepsin 5mg / mL, NaCl 0.5% aseptically, and prepare simulated gastric juice. Culture at a suitable temperature, and calculate the number of viable bacteria by plate counting.

[0034] (5) Preparation of different bile salt concentrations

[0035] Pig bile salts were added to the MRS liquid medium to make the concentrations 0% (control), 0.15%, and 0.3%, respectively. The activated lactic acid bacteria strains were inoculated in the above-mentioned liquid medium with different concentrations according to the inoculation amount of 1%, and cultured at a suitable temperature, and the number of viable bacteria was calculated by plate counting method.

[0036] (6) Preparation of artificial intestin...

Embodiment 3

[0046] Embodiment 3: the preparation of lactic acid bacteria preparation

[0047]1. Prepare lactic acid bacteria preparations by spray drying technology, use gum arabic as the wall material for spray drying, set the inlet temperature at 160°C, the wall material concentration at 20%, the feed rate at 400mL / h and the wall material ratio at 1:9, the viable bacteria after spraying The number was 8.70 log CFU / g.

[0048] 2. Determination of the embedding rate and stability of lactic acid bacteria preparations

[0049] The embedding rate of microcapsules is one of the important indicators for evaluating the quality of microcapsules, which refers to the ratio of the number of viable bacteria in the microcapsules to the number of viable bacteria in the product. The embedding rate of lactic acid bacteria preparation was 72.18%.

[0050] Stability is an important index in the quality evaluation of microcapsules containing live bacteria preparations. In order to evaluate the shelf stab...

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Abstract

The invention relates a Lactobacillus acidophilus and an application thereof. The Lactobacillus acidophilus is Lactobacillus acidophilus FZU-LA1301X with the preservation number of CGMCC No.12453, and is preserved in China General Microbiological Culture Collection Center on May 16, 2016. The Lactobacillus acidophilus FZU-LA1301X can be colonized in weaned piglets, has strong tolerance to gastric acid, bile salt and intestinal juice, has a good live bacterium number in an improved fermentation medium, has a high survival rate after being spray-dried, and can be used in animal feed additives.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a lactobacillus acidophilus and its application. technical background [0002] At present, the method of adding antibiotic drugs to feed is generally used to prevent animal diarrhea, but antibiotics will destroy the micro-ecological balance of the digestive tract, and also lead to a decline in immune function, and are likely to remain in animal products, posing a great threat to human health. Therefore, antibiotic substitutes with no residue, no toxic side effects, and no drug resistance—probiotics emerged as the times require. Microbial feed additives have the functions of maintaining animal intestinal health, relieving adverse stress, regulating body metabolism and improving the quality of livestock products. In the feed industry, people regard probiotics as one of the most effective alternatives to antibiotics, hoping to produce safe livestock products through probiotics and at t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30C12R1/23
CPCC12N1/20C12N1/205C12R2001/23
Inventor 倪莉饶甜甜刘志彬张雯
Owner XIAMEN LONGLI IND & TRADE
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