33results about How to "Has an immunosuppressive effect" patented technology

The invention provides a preparing method of a compound FK506-A, which includes the following steps: a) heating FK506 for 2 to 25 minutes under 110 to 150 degrees C, so as to obtain a FK506-A crude product; b) adding organic solvent into the FK506-A crude product, adding silica gel after dissolving, and drying after uniformly stirring, so as to obtain a dry sample; and c) adding the dry sample into a chromatographic column filled with silica gel and eluting by adding eluting solvent, and collecting the eluent with the FK506-A purity determined by HPLC to be no less than 75% and concentrating to be dry solid, so as to obtain FK506-A pure product by crystallizing the solid. The preparing method of compound FK506-A has the advantages that the operation is simple, and the industrial production is easy.

The invention relates to a faded shewanella oneidensis LCT-SM262 strain in a space environment, and biological characteristics, a genomic deoxyribonucleic acid (DNA) sequence, a transcriptome and differential proteomics of the faded shewanella oneidensis LCT-SM262 strain, and in particular relates to a functional sequence and a molecule which are analyzed and identified through comparing with ground faded shewanella oneidensis. The mutant gene, differential expression gene and protein molecule of the faded shewanella oneidensis in the space environment can be comprehensively and accurately obtained, so that researches on the function and mechanism of the space environment on microorganisms are facilitated, and the safety of the space environment is guaranteed; novel toxicity and drug-resistant target spots can be conveniently found out, so that novel treatment target spots are provided for clinic infectious diseases; and a metabolic product-prodigiosin of the strain has functions of tumor resisting and immunosuppression and has a potential application prospect.

The invention provides a vaccine composition, which comprises live porcine reproductive and respiratory syndrome virus antigen and immune dose live virus antigen of porcine pseudorabies virus, and a freeze-drying protective agent. The vaccine composition provided by the invention has good immunogenicity and safety, and the vaccine composition solves the immunosuppressive problem of porcine reproductive and respiratory syndrome antigen and porcine pseudorabies antigen under the condition that the antigen content is relatively low , has a significant immune protection effect on the prevention of porcine reproductive and respiratory syndrome and porcine pseudorabies.

The invention relates to an in-vitro amplification method of cord blood Treg cells, comprising the steps of S1, isolating mononuclear cells; S2, adjusting cellular density, inoculating to a culture flask, and adding inactivated plasma; on the first day, adding coumestrol, TGF-beta, CD3 / CD28 monoclonal antibody and IL-2; S3, on the fourth day, supplementing a culture medium, adding inactivated plasma, coumestrol and TGF-beta, and keeping the final concentration constant; S4, on the sixth day, supplementing the culture medium, and keeping the final concentrations of other components unchanged; S5, on the ninth day, supplementing the culture medium, and keeping the final concentrations of other components unchanged; S6, on the twelfth day, collecting cells. The amplification of CD4, CD25 and Tregs is induced under the coaction of coumestrol and TGF-beta, the amplifying efficiency is high, and high-purity Treg cells can be acquired without pre-purification.

The invention relates to medicine for restoring wound, in particular to a preparation method of a novel medical cell repairing agent. The preparation method has the concrete preparation steps of separation purification and culture of human amniotic mesenchymal stem cells, preparation of a sodium alginate complexing agent and preparation of a cell restoring agent. The invention builds the preparation method of human amniotic mesenchymal stem cells and a corresponding stem cell bank, and makes the preparation for large-scale production and preparation of the human amniotic mesenchymal stem cellrepairing agent and the application of the repairing agent.

An alkannin derivative as immuno-suppressive agent and its metallic complex compound with a chemical constitution formulas III and IV disclosed in the specification, wherein in formula III, R is C1-6 saturated, unsaturated and aromatic acyl, in formula IV, R is hydrogen, C1-6 saturated, unsaturated and aromatic acyl, M is metallic Cu, Ca, Zn. The present invention realizes immunological suppression effect, thus can be used for treating diseases relating to human body autoimmune including chronic infectious arthritis, scleredema, lupus erythematosus, HIV infection and malignant tumor.

The invention belongs to the field of Chinese medicament, and provides a Chinese medicinal composition for treating prostatitis or prostate hyperplasia and a preparation method for the Chinese medicinal composition. The Chinese medicinal composition is prepared from the following raw materials in portion by weight: 1 to 5 portions of rape pollen and 1 to 5 portions of wild chrysanthemum, wherein the optimal dose is based on the weight ratio of the rape pollen to the wild chrysanthemum of 1 to 1. The Chinese medicinal composition has quite remarkable effect of treating the prostatitis and the prostate hyperplasia. The preparation method can effectively extract effective components of the medicament, remove impurities and well exert medicinal effect.

The invention relates to a drug and specifically relates to a drug compound. The drug compound contains taxus mairei extracts and a pharmaceutically acceptable carrier, wherein the weight percent of the taxus mairei extracts is 0.1-99.9%. The resources of the drug compound are natural; the preparation technique is simple; the toxicity to cells is low; the immunosuppressive action is higher; the application prospect is wide.

A medical application of periplocoside A and E in preparing the immunodepress ant with high effect and low poison is disclosed.

The invention relates to a biomimetic nanoparticle coated with a mesenchymal stem cell membrane with PD-L1 molecules overexpressed on the surface, and its preparation and application. The mesenchymal stem cell membrane-coated biomimetic nanoparticle with PD-L1 molecule overexpression on the surface of the present invention includes a nano-core and a mesenchymal stem cell membrane wrapped outside the nano-core; the nano-core includes a biocompatible polymer, The membrane surface of mesenchymal stem cell membranes overexpresses PD‑L1 molecules. The present invention also discloses the application of the membrane-coated biomimetic nanoparticles of mesenchymal stem cells overexpressed on the surface of PD-L1 molecules in the preparation of inflammation treatment preparations. The biomimetic nanoparticle of the present invention has significant immunosuppressive effect, good biocompatibility, simple and mature preparation process, and can be used to prepare inflammation treatment preparations, which can be effectively enriched in the inflammatory site, by inhibiting the excessively activated immune response of the inflammatory site and cytokine storm, showing excellent curative effect in the treatment of inflammation.

The invention relates to a composition and a method conducive to acquiring follicular regulatory T cells in vitro and an application of the composition. The invention proves that differentiation and number proliferation of in-vitro Tfr (follicular regulatory T cells) can be effectively induced by the aid of irritation of TGF (transforming growth factor)-beta, IL (interleukin)-2, anti-CD3 antibodies, anti-CD28 antibodies and baicalin, and expression of Foxp3 in the Tfr is facilitated. The Tfr cells amplified by the method can be used for treating autoimmune diseases and chronic inflammatory diseases, the autoimmune diseases include lupus erythematosus, dermatomyositis, vasculitides, sicca syndrome, scleroderma, rheumatoid arthritis, ankylosing spondylitis, disseminated sclerosis and autoimmune hepatitis, and the chronic inflammatory diseases include diabetes, coronary heart diseases, hyperlipemia, eczema, vitiligos, atopic dermatitis, lichen planus and the like.