44results about How to "Effective differentiation" patented technology

The invention relates to a method for inducing mesenchymal stem cells to be differentiated into pancreatic island beta-like cells and application thereof. The method comprises the following three stages: firstly, a double-gene expression vector containing an NKX6.1 gene and a PDX1 gene is used to infect the mesenchymal stem cells to transfer double genes into the stem cells and express the double genes, the cells are cultivated, and the double genes are confirmed to be expressed for 4 to 7 days; secondly, a culture liquid containing a human epithelial growth factor, a human basic fibroblast growth factor, and B27 is used to continuously induce for 3 to 4 days; and finally a culture medium containing glucagon-like peptide-1, human beta cytosine, a hepatic cell growth factor, niacinamide, B27, beta-mercaptoethanol, and FCS is used to continuously induce for 7 to 10 days. The pancreatic island beta-like cells obtained by the method can be used to produce insulin, serve as seed cells for cellular transplantation to treat diabetes, and has huge economic and social significance.

The invention relates to a crystal glass production and manufacturing assembly line. A crystal glass dosing device doses different material components of crystal glass proportionally, the dosed material components of the crystal glass are dosed, taken out and conveyed to a feed port of a crystal glass melting device to be heated and melted, a heated and melted crystal glass liquid enters a fixingring of a bubble clarifying device through a discharge port, the crystal glass liquid is heated, a bubble of the crystal glass liquid is clarified, the crystal glass liquid with the bubble clarified enters a pressing forming machine through a glass liquid outlet to be preliminarily pressed and formed, a preliminarily pressed and formed crystal glass plate is conveyed through a grinding groove to acrystal glass polishing device, the surface of the crystal glass plate is grinded and polished, the crystal glass plate is conveyed to a working platform after being grinded and polished, and the edge of the crystal glass plate is finished through a crystal glass edge finishing device.

It is intended to provide a medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes and a method of redifferentiating dedifferentiated chondrocytes into chondrocytes whereby dedifferentiated chondrocytes, which have been dedifferentiated by, for example, culturing in vitro and thus show weakened chondral characteristics, can be efficiently and easily redifferentiated into the original chondrocytes. That is to say, a medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes which is to be used for redifferentiating dedifferentiated chondrocytes having been dedifferentiated and thus showing weakened chondral characteristics into the original chondrocytes and contains insulin together with at least one member selected from among BMP-2 and its analogs; and a method of redifferentiating dedifferentiated chondrocytes into the original chondrocytes by culturing the dedifferentiated chondrocytes with the use of the medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes. In a preferred embodiment, the above-described medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes further contains T3.

The invention provides an application of a method for the autocrine secretion of an extracellular matrix by stem cells and the induction of the stem cells to become hepatocytes. The extracellular matrix is secreted by inducing bone marrow mesenchymal stem cells and removes the matrix of the bone marrow mesenchymal stem cells. The invention concretely provides the method for inducing the bone marrow mesenchymal stem cells into hepatocytes by the extracellular matrix according to actual demands. The method for generating the hepatocytes through induction has the advantages of high efficiency, practicality, high induction efficiency and good differentiation effect, and the glycogen synthesis capability and the urea synthesis capability of the hepatocytes obtained after the induction are strong.

The invention discloses an eccrine sweat gland cell induction medium and an application thereof. The eccrine sweat gland cell induction medium comprises a DMEM / F12 cell medium, a keratinocyt medium, fetal calf serum, an epidermal growth factor, triiodothyronine, hydrocortisone, insulin-transferrin-sodium selenite, L-glutamine, penicillin, streptomycin, a hepatocyte growth factor and a bone morphogenetic protein 4. The eccrine sweat gland cell induction medium disclosed by the invention can be used for successfully inducing stem cells to be directionally differentiated to eccrine sweat gland-like cells so as to provide sufficient eccrine sweat gland cells for treating extensive burn patients, so that the living quality of the patients is greatly improved. The medium further provides a theoretical basis for researching differentiated development of the sweat gland, so that the medium has a potential clinical application prospect.

The invention discloses a culture solution for inducing multifunctional stem cells to differentiate into dopamine progenitor cells and a differentiation method. The culture solution comprises a DMEM / F12 culture medium and a Neurobaal culture medium in a volume ratio of 1: (0.9-1.3), and an N2 supplement, a B27 supplement, Lascorbic acid, Lglutamine, a GSK3 beta inhibitor, a TGF beta inhibitor, a BMP inhibitor, a fibroblast growth factor, a human leukemia inhibition factor hLIF, heparin Heparin and a nutritional factor required for growth of dopaminergic progenitor cells. The culture solution disclosed by the invention can be used for obtaining dopamine progenitor cells by taking induced multifunctional stem cells as raw materials through directional differentiation culture, can be used for direct transplantation or final differentiation into dopamine neurons, and can be used for clinical cell substitution transplantation treatment of PD, in-vitro disease modeling research, drug screening and other biomedical applications. The method has the advantages of being convenient to operate, short in culture time, high in yield, high in differentiation purity, free of animal-derived components in the whole link and the like.

The invention provides a seedling raising method of Capsicum annuum L. var. conoides (Mill.) Irish. The method comprises the following steps: seed treatment and germination, seedling raising preparation, seedling raising management and transplant cultivation. According to the planting method, the germination rate and the survival rate are improved though disinfection and germination treatment of Capsicum annuum L. var. conoides (Mill.) Irish seeds, and through special seedling substrate seedling raising and strict seedling raising management; and the purposes of biomass recycling and seedling-raising cost reducing are realized through rational utilization of crop straws.

The invention relates to the technical field of river channel treatment, and discloses an efficient desilting device for water conservancy river channel project treatment. The device comprises a ship body, wherein a chain bucket machine is fixedly connected to the top part of the right end of the ship body; a bucket is arranged at the bottom end of the chain bucket machine, and a grating is fixedly connected to the inner wall of the bucket; a box body is arranged at the inner bottom part of the ship body; a primary filtering area, a first-stage precipitating area and a second-stage precipitating area are sequentially arranged in the box body from right to left; and a pre-precipitating groove is formed in the bottom part of the primary filtering area and communicates with the first-stage precipitating area. According to the device, the second-stage precipitating area and a water collecting groove are arranged, so that the advantage of greatly reducing water pollution is realized; river water and silt can be effectively separated after being treated in the second-stage precipitating area, and thus the silt can be quickly settled; after being separated through an inclined pipe separating assembly, the separated river water is collected into the water collecting groove, so that reasonable output water quality is ensured.

The invention discloses an oil-control conditioning composition containing plant extractives, and aim to provide the oil-control conditioning composition, containing the plant extractives, which is natural, free of stimulation, safe and gentle and can effectively balance sebum secretion and shrink pores. The composition is mainly prepared from the following compounds in parts by weight: 6 to 15 parts of a guava fruit extractive, 4 to 10 parts of a populus tremula bark extractive, 2 to 10 parts of a flaxseed extractive and 3 to 8 parts of a barosma betulina leaf extractive. The oil-control conditioning composition belongs to the technical field of cosmetics.

The invention provides application of ZDHHC21 genes in preparation of leukemia induced differentiation therapy drugs, and application of polypeptides targeting the DHHC21 genes, small molecule inhibitors and interfering siRNAs in preparation of leukemia induced differentiation agents. Research shows that as the ZDHHC21 amount is reduced by the RNA interfering technology, the proliferation of leukemia HL60 and NB4 cells is significantly inhibited, the expression of cell surface differentiation-specific antigens CD11b is significantly increased, the reduction ability of NBT of the cells is enhanced, and the ratio of nucleus to cytoplasm in the cells is reduced, the shapes of cell nuclei are changed into U shapes or half-moon shapes. Down-regulation of ZDHHC21 can effectively induce the differentiation of the human leukemia cells, a new direction is provided for the development of treatment drugs for leukemia, a new therapeutic target is provided for induction differentiation therapy of leukemia, and a new research field is opened up for the treatment of leukemia, the improvement of the treatment effect and the improvement of drug resistance.

The invention relates to a composition and a method conducive to acquiring follicular regulatory T cells in vitro and an application of the composition. The invention proves that differentiation and number proliferation of in-vitro Tfr (follicular regulatory T cells) can be effectively induced by the aid of irritation of TGF (transforming growth factor)-beta, IL (interleukin)-2, anti-CD3 antibodies, anti-CD28 antibodies and baicalin, and expression of Foxp3 in the Tfr is facilitated. The Tfr cells amplified by the method can be used for treating autoimmune diseases and chronic inflammatory diseases, the autoimmune diseases include lupus erythematosus, dermatomyositis, vasculitides, sicca syndrome, scleroderma, rheumatoid arthritis, ankylosing spondylitis, disseminated sclerosis and autoimmune hepatitis, and the chronic inflammatory diseases include diabetes, coronary heart diseases, hyperlipemia, eczema, vitiligos, atopic dermatitis, lichen planus and the like.

The invention discloses a method for promoting cartilage tissue differentiation of mesenchymal stem cells. The method comprises the following steps: separating the human mesenchymal stem cells; then, implementing stable over-expression of NR2F2 genes in the mesenchymal stem cells by virtue of lentivirus and down-regulating the expression of the NR2F2 genes by virtue of a small RNA interference technology; cultivating 3D cell microspheres after the over-expression of the NR2F2 genes in the mesenchymal stem cells and down-regulating the expression; mixing the human mesenchymal stem cells, polyethylene glycol dimethacrylate, acrylate peptide, a 2959 photoinitiator and PBS (phosphate buffered saline), so that biological ink is prepared; printing 3D hydrogel cartilage tissues, which achieve the over-expression of the NR2F2 genes, on biological printing paper by virtue of a biological printer; and cultivating the 3D hydrogel cartilage tissues subcutaneously into a mouse by virtue of a cartilage forming medium for 21d, so that joint cartilage tissues are formed. The method provided by the invention is simple and convenient to operate and is strong in repeatability; and the method can effectively promote the cartilage tissue differentiation of the mesenchymal stem cells, so that beneficial reference is provided for the scientific researches of cartilage tissue engineering and the clinical treatment of cartilage injuries.

The invention discloses a synthetic adhering culture medium for cell culture. Each liter of synthetic adhering culture medium is prepared from components as follows: 0.08 g of biotin, 20 mu g of corticosterone, 2 g of L-carnitine, 1.2 g of linoleic acid, 1.2 g of ethanolamine, 0.8 g of linolenic acid, 13-17 g of D-glucose, 5 mu g of progesterone, 1.2 g of sodium pyruvate, 0.08 g of retinyl acetate, 4 g of catalase, 0.9-1.2 g of folic acid, 0.8-1.3 g of reduced glutathione, 60-70 mu g of lipoic acid, 2-3 g of transferrin, 6-8 g of human insulin, 0.1 mg of sodium selenite, 500-600 mL of a banana peel extract, 1.2-2 g of naphthylacetic acid and 0.8-1.5 g of vitamin C. According to the synthetic adhering culture medium for cell culture and a preparation method of the culture medium, no animal serum is contained, the stem cell differentiation culture time is greatly shortened and the experiment cost is saved.

The invention relates to the field of cells, in particular to a composition, an inducing preparation containing the composition and an inducing method. The composition is prepared from 5-azacytidine, bone morphogenetic protein and angiotensin II. The invention further provides an inducing preparation. In the inducing preparation, the final concentration of 5-azacytidine is 5-15 micromole / L, the final concentration of the bone morphogenetic protein is 5-15 mcg / L, and the final concentration of the angiotensin II is 1-10 micromole / L. The invention provides the method for effectively inducing GMSCs into cardiomyocyte. A DMEM / F12 culture medium with the culture solution components comprising 10% of FBS, 10 micromole / L of 5-aza, 10 ng / mL of BMP-2 and 5 micromole / L of Ang-II is subjected to induction differentiation. By means of the method, the GMSCs can be effectively induced to cardiomyocytes differentiation. The materials of the GMSCs are convenient to obtain, in-vitro expansion is easy, the GMSCs can be used for autotransplantation, and therefore an immunological rejection reaction is avoided.

The invention relates to a method for cultivating cordyceps sinensis by taking hawthorn as a main substrate. The method includes the steps of inoculating a multi-stage domesticated cordyceps sinensis strain into a culture medium consisting of hawthorn fruits, hawthorn branches and leaves and sticky rice as main raw materials, and cultivating high-quality cordyceps sinensis sporocarp suitable for well growing in the hawthorn substrate. According to the method for cultivating the cordyceps sinensis by taking the hawthorn as the main substrate, hawthorn fruit residues, the crushed hawthorn branches and leaves and the sticky rice form the culture medium which is rich and comprehensive in nutrition, so that cordyceps sinensis hyphae grow rapidly and strongly under nutrition nourishing, cordyceps sinensis sporocarp also grow strongly, and the biological efficiency is high. According to the method, training and domestication of several stages are carried out on a cordyceps sinensis test tube strain, a cordyceps sinensis liquid strain and a cordyceps sinensis sporocarp cultivation process, domestication is carried out mainly from gradual increase of hawthorn inclusions in the culture medium and gradual reduction of PH, and the cordyceps sinensis hyphae grow in the hawthorn culture medium from gradual adaptation to complete adaptation, so that high-quality and high-yield cordyceps sinensis sporocarp can finally grow in the hawthorn culture medium.

The invention discloses an application of tissue-engineering bone in preparation of medicines, wherein the medicine is used for treating bone injury diseases. The tissue-engineering bone includes mesenchymal stem cells and pre-induction cells. The tissue-engineering bone has a strong capability of being differentiated into bone cells, is high in yield of the bone cells, and can provides required cells for tissue repair in injured parts of bone and quickly repair and cure the injured parts of bone. The tissue-engineering bone is convenient to prepare and is safe and reliable.

The invention discloses a method for promoting cartilage tissue differentiation of mesenchymal stem cells. The method comprises the following steps: separating the human mesenchymal stem cells; then, implementing stable over-expression of NR2F2 genes in the mesenchymal stem cells by virtue of lentivirus and down-regulating the expression of the NR2F2 genes by virtue of a small RNA interference technology; cultivating 3D cell microspheres after the over-expression of the NR2F2 genes in the mesenchymal stem cells and down-regulating the expression; mixing the human mesenchymal stem cells, polyethylene glycol dimethacrylate, acrylate peptide, a 2959 photoinitiator and PBS (phosphate buffered saline), so that biological ink is prepared; printing 3D hydrogel cartilage tissues, which achieve the over-expression of the NR2F2 genes, on biological printing paper by virtue of a biological printer; and cultivating the 3D hydrogel cartilage tissues subcutaneously into a mouse by virtue of a cartilage forming medium for 21d, so that joint cartilage tissues are formed. The method provided by the invention is simple and convenient to operate and is strong in repeatability; and the method can effectively promote the cartilage tissue differentiation of the mesenchymal stem cells, so that beneficial reference is provided for the scientific researches of cartilage tissue engineering and the clinical treatment of cartilage injuries.

The invention provides a differentiation culture method for directionally inducing mesenchymal stem cells. The method comprises the following steps of: (1) taking mesenchymal stem cells, and putting the mesenchymal stem cells in a serum-free culture medium to perform serum starvation treatment for 12-24 hours; and putting the mesenchymal stem cells in a culture medium A to perform preculture for 5-6 hours; and (2) after the preculture finishes, transferring the mesenchymal stem cells into a culture medium B to perform culture for 2-4 weeks, wherein the culture medium A uses a DMEM / F12 culturemedium as a basal culture medium, and 5-10 micromoles of very low density lipoprotein, 1-3nmol of fibroblast growth factor-2 and 8-12nmol of L-glutamine are added. The method can effectively enhance the ALP level of the induced cells, and can obviously enhance the expression level of Runx2, thereby effectively and directionally inducing the mesenchymal stem cells to differentiate into osteoblasts.

The invention provides a culture medium for inducing differentiation of dental pulp stem cells into adipoblasts, a kit containing the culture medium and application of the kit. The culture medium comprises a basic culture medium, insulin, a platelet lysis buffer, indometacin, dexamethasone and 3-isobutyl-1-methylxanthine. The components contained in the culture medium have a good synergistic effect, so induced differentiation from the dental pulp stem cells to the adipocytes can be achieved, the adipogenic directional differentiation specificity of the culture medium is high, the specificity of the culture medium is high, time needed for the adipogenic induced differentiation of the dental pulp stem cells can be greatly shortened, and induced differentiation efficiency is improved. Meanwhile, the culture medium is applied to the kit for inducing the differentiation of the dental pulp stem cells into the adipoblasts. The kit is used for inducing the dental pulp stem cells, and stable and efficient induced differentiation of the dental pulp stem cells into the adipoblasts can be achieved.

The invention belongs to the technical field of animal cell culturing, and particularly relates to a method of forming a skeletal muscle cell by induction differentiation by utilization of a pig dedifferentiated adipocyte. A mature adipocyte of pig subcutaneous adipose tissues is separated by a collagenase digestion method, and is dedifferentiated to form the dedifferentiated adipocyte (DFAT cell). The DFAT cell has functions similar to that of mesenchymal stem cells (MSCs) and can be efficiently multiplicated in vitro and can introduce multi-directional differentiation. In addition, by utilization of induction of the galectin-1 protein in vitro, the pig DFAT cell is dedifferentiated to form a multinucleate cell capable of expressing a skeletal muscle cell specific marker molecule.

An exemplary embodiment of the present invention provides TSP-1, TSP-2, IL-17BR and HB-EGF associated with stem cell activities and applications thereof.

The invention discloses application of an AK2 gene in preparation of a leukemia induced differentiation treatment medicine. According to the invention, the differentiation of leukemia cells can be directly induced by reducing the expression of AK2 by using an siRNA interference technology. Therefore, it is proposed for the first time that the AK2 gene is a key gene for leukemia induced differentiation treatment, siRNAs molecules of the AK2 can be used as a leukemia induced differentiation agent, a new treatment target is provided for induced differentiation treatment of leukemia, and the AK2 gene plays an important role in treatment of leukemia. The invention provides possibilities for preparing novel drugs for induced differentiation of leukemia, improving the curative effect of leukemia patients and improving drug resistance and related prognosis conditions.

The invention relates to a method for inducing mesenchymal stem cells to be differentiated into pancreatic island beta-like cells and application thereof. The method comprises the following three stages: firstly, a double-gene expression vector containing an NKX6.1 gene and a PDX1 gene is used to infect the mesenchymal stem cells to transfer double genes into the stem cells and express the doublegenes, the cells are cultivated, and the double genes are confirmed to be expressed for 4 to 7 days; secondly, a culture liquid containing a human epithelial growth factor, a human basic fibroblast growth factor, and B27 is used to continuously induce for 3 to 4 days; and finally a culture medium containing glucagon-like peptide-1, human beta cytosine, a hepatic cell growth factor, niacinamide, B27, beta-mercaptoethanol, and FCS is used to continuously induce for 7 to 10 days. The pancreatic island beta-like cells obtained by the method can be used to produce insulin, serve as seed cells for cellular transplantation to treat diabetes, and has huge economic and social significance.

The invention relates to the technical field of river course management, and discloses a high-efficiency dredging device for water conservancy river project treatment, including a hull, the top of the right end of the hull is fixedly connected with a chain bucket machine, and the bottom end of the chain bucket machine is equipped with A bucket, the inner wall of the bucket is fixedly connected with a grid, the inner bottom of the hull is provided with a box, and the inside of the box is sequentially provided with a primary filter area, a primary sedimentation area and a secondary filter area from right to left. In the sedimentation area, a pre-sedimentation tank is provided at the bottom of the primary filtration area, and is communicated with the primary sedimentation area through the pre-settling tank. The high-efficiency dredging device for water conservancy and river project control has achieved the advantages of greatly reducing water pollution through the secondary sedimentation area and the sump. Through the treatment in the secondary sedimentation area, it can effectively decompose the river water and silt, so that the silt can be quickly recovered. Settling, and after the separation, the river water is separated by the inclined tube separation component and then poured into the sump, which can ensure reasonable effluent water quality.

The invention relates to a detection method for a compromised node in a wireless sensor network. It analyzes and processes the fictitious false data packet loss attack of the compromised node in the forwarding process of the SEF model data packet, and proposes a trapping mechanism on the way. The opportunity for compromised nodes to make mistakes can effectively differentiate compromised nodes and normal nodes, and detect compromised nodes in wireless sensor networks based on differentiated compromised nodes and normal nodes, and can detect compromised nodes that launch fictitious false packet loss attacks, Indirectly solves the false packet loss attack. In addition, the invention also relates to a detection device for a compromised node in a wireless sensor network.

The invention relates to a skin care product composition with moisturizing and wrinkle-resistant effects and application of the skin care product composition. The skin care product composition is prepared from the following ingredients in parts by weight: 5*10<-7> to 1*10<-5> part of a human stem cell factor, 1-5 parts of collagen, 0.03-2 parts of chitosan and 0.2-1 part of sodium hyaluronate. Theskin care product composition has excellent moisturizing and wrinkle-resistant effects, the stem cell factor is applied in an externally-used skin care product form, and the adverse effect caused byinjection is avoided.