Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composition, inducing preparation containing composition and inducing method

A technology of composition and preparation, which is applied in the field of induction preparation and induction, and can solve the problems of few research reports

Inactive Publication Date: 2017-03-15
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few reports on the induction of GMSCs differentiation into cardiomyocytes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition, inducing preparation containing composition and inducing method
  • Composition, inducing preparation containing composition and inducing method
  • Composition, inducing preparation containing composition and inducing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 1. Primary isolation and culture of GMSCs

[0059] 1) Source of raw materials: The gingiva removed by clinical orthodontic eruption or impacted tooth extraction is used to remove the gingival tissue surrounding the permanent teeth. Patients are required to have no gingival hyperplasia, inflammation and use of drugs that cause gingival hyperplasia. The excised gingival tissue was quickly immersed in 4°C pre-cooled PBS containing 3 times double antibody.

[0060] Among them, the 3-fold double antibody concentration was 300u / mL penicillin and 300u / mL streptomycin.

[0061] 2) Rinse: Rinse 3 times with PBS containing 3 times the double antibody, and then soak the gingiva in the serum-free medium of mesenchymal stem cells for 5 minutes;

[0062] Among them, the 3-fold double antibody concentration was 300u / mL penicillin and 300u / mL streptomycin.

[0063] Wherein, the serum-free medium of mesenchymal stem cells contains 100u / mL penicillin and 100u / mL streptomycin.

[0064...

Embodiment 2

[0083] Embodiment 2 GMSCs differentiation

[0084] In order to verify the effect of differentiation induction of the present invention, set 4 groups of control groups and 4 groups of experimental groups at the same time, select the 3rd generation GMSCs, press 1×10 4 The cells / mL density was inoculated in a six-well plate with polylysine-treated sterile coverslips, and 2 mL of DMEM / F12 medium containing 10% FBS was added to each well. After 24 hours, the corresponding culture medium of each group was replaced, at 37°C, 5% CO 2 , Culture in saturated humidity, and replace with new culture medium every 2 to 3 days.

[0085] Control group 1 is a negative control group, which is a conventional culture group, and the culture medium used is: DMEM / F12 culture medium containing 10% FBS by volume.

[0086] Control group 2 is a positive control group, and the induction medium used is: DMEM / F12 culture medium with 10% FBS and 10 μmol / L 5-aza.

[0087] Control group 3 is a positive cont...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of cells, in particular to a composition, an inducing preparation containing the composition and an inducing method. The composition is prepared from 5-azacytidine, bone morphogenetic protein and angiotensin II. The invention further provides an inducing preparation. In the inducing preparation, the final concentration of 5-azacytidine is 5-15 micromole / L, the final concentration of the bone morphogenetic protein is 5-15 mcg / L, and the final concentration of the angiotensin II is 1-10 micromole / L. The invention provides the method for effectively inducing GMSCs into cardiomyocyte. A DMEM / F12 culture medium with the culture solution components comprising 10% of FBS, 10 micromole / L of 5-aza, 10 ng / mL of BMP-2 and 5 micromole / L of Ang-II is subjected to induction differentiation. By means of the method, the GMSCs can be effectively induced to cardiomyocytes differentiation. The materials of the GMSCs are convenient to obtain, in-vitro expansion is easy, the GMSCs can be used for autotransplantation, and therefore an immunological rejection reaction is avoided.

Description

technical field [0001] The invention relates to the field of cells, in particular to a composition, an induction preparation containing the composition and an induction method. Background technique [0002] Myocardial infarction is the myocardial damage caused by the imbalance between coronary blood flow and myocardial demand caused by changes in coronary circulation. It is a serious ischemic heart disease clinically. Cardiomyocytes after necrosis are gradually replaced by scar tissue. Due to the lack of elasticity of scar tissue, it is difficult to meet the requirements of cardiac contraction and relaxation. In order to compensate for the loss of cardiomyocyte function, the heart gradually undergoes degenerative left ventricular remodeling, and cardiac function decline, eventually leading to congestive heart failure and even sudden death. Although clinical drugs and interventional therapy can improve symptoms, they cannot restore damaged cardiomyocytes. Although heart tra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/077
CPCC12N5/0657C12N2500/40C12N2501/155C12N2501/32C12N2506/1361
Inventor 葛啸虎陈海佳王一飞戚康艺
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com