Mesenchymal stem cells and exosomes of mesenchymal stem cells and application of mesenchymal stem cells in treating anal fistula
A technology of mesenchymal stem cells and exosomes, applied in the field of stem cells, can solve problems such as differences in curative effects and unstable cell survival rates
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Embodiment 1
[0023] A mixed preparation for treating anal fistula, comprising the following active ingredients: mesenchymal stem cells (ADSCs), exosomes of mesenchymal stem cells (EXO-ADSCs), autologous platelet-rich plasma (PRP) and synthetic antibacterial polypeptide (AMP).
[0024] The preparation method of the described PRP comprises the following steps: extracting 35ml of peripheral blood from the patient, adding 8ml of anticoagulant, transferring to a 50ml centrifuge tube, centrifuging at 300g / min for 25min, absorbing the upper plasma, middle buffy coat and a small amount of red blood cells to the In a new 50ml centrifuge tube; then centrifuge at 750g / min for 10min, suck out the supernatant to a new centrifuge tube for later use, use the supernatant to adjust the platelet concentration to 800×10 6 Each / mL is PRP.
[0025] The method for extracting exosomes from mesenchymal stem cells comprises the following steps: take the culture supernatant of the amplified third-generation mesench...
Embodiment 2
[0031] A mixed preparation for treating anal fistula, comprising the following active ingredients: mesenchymal stem cells (ADSCs), exosomes of mesenchymal stem cells (EXO-ADSCs), autologous platelet-rich plasma (PRP) and synthetic antibacterial polypeptide (AMP).
[0032] The preparation method of the described PRP comprises the following steps: extracting 50ml of peripheral blood from the patient, adding 2ml of anticoagulant, transferring to a 50ml centrifuge tube, centrifuging at 300g / min for 30min, absorbing the upper plasma, the middle buffy coat and a small amount of red blood cells to the In a new 50ml centrifuge tube; then centrifuge at 700g / min for 10min, suck out the supernatant to a new centrifuge tube for later use, use the supernatant to adjust the platelet concentration to 1000×10 6 Each / mL is PRP.
[0033] The method for extracting exosomes from mesenchymal stem cells comprises the following steps: take the culture supernatant of the amplified third-generation me...
Embodiment 3
[0039] A mixed preparation for treating anal fistula, comprising the following active ingredients: mesenchymal stem cells (ADSCs), exosomes of mesenchymal stem cells (EXO-ADSCs), autologous platelet-rich plasma (PRP) and synthetic antibacterial polypeptide (AMP).
[0040] The preparation method of described PRP comprises the following steps: extracting 20ml of peripheral blood from the patient himself, adding 10ml of anticoagulant, transferring to a 50ml centrifuge tube, centrifuging at 300g / min for 20min, absorbing the upper plasma, the middle buffy coat and a small amount of red blood cells to the In a new 50ml centrifuge tube; then centrifuge at 800g / min for 10min, suck out the supernatant to a new centrifuge tube for later use, use the supernatant to adjust the platelet concentration to 500×10 6 Each / mL is PRP.
[0041] The method for extracting exosomes from mesenchymal stem cells comprises the following steps: take the culture supernatant of the amplified third-generatio...
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