40results about How to "Fewer reaction by-products" patented technology

The invention discloses application of heat-resistant beta-glucosidase and mutants thereof. The mutants of the heat-resistant beta-glucosidase G224T, N223S and N223S / G224T have the amino acid sequences shown in SEQ: ID NO. 1, SEQ: ID NO. 2 and SEQ: ID NO. 3; the heat-resistant beta-glucosidase and the mutants thereof can be used for preparing quercetin through enzymolysis of quercetin glycoside. The preparation of the quercetin through the heat-resistant beta-glucosidase and the mutants thereof has the advantages of high conversion efficiency, few reaction byproducts, easy separation and purification of the product, less chemical pollution, and high yield and high purity of the obtained quercetin product, and the like.

The invention discloses an application of heat-resisting beta-glucosidase mutants N223T, G224F and N223T / G224F to transforming arctiin into arctigenin. Amino acid sequences of the heat-resisting beta-glucosidase mutants N223T, G224F and N223T / G224F are indicated in SEQ: ID NO.1, ID NO.2 and ID NO.3. The arctiin is hydrolyzed to prepare the arctigenin by the aid of heat-resisting beta-glucosidase and the mutants thereof, and as compared with wild-type enzyme, the heat-resisting beta-glucosidase has the advantages of high transformation efficiency, fewer reaction by-products, high yield and purity, easiness in separation and purification, less chemical pollution and the like.

The invention relates to application of a transition metal carbonate nanomaterial in electrocatalytic reduction of nitrate, belonging to the technical field of electrocatalytic reduction of nitrate. The preparation method comprises the following steps: preparing the transition metal carbonate nanomaterial Co@Cu2(OH)2CO3 by a simple one-step hydrothermal method, preparing catalyst slurry from the transition metal carbonate nanomaterial Co@Cu2(OH)2CO3, acetylene black and an N-methyl pyrrolidone solution of polyvinylidene fluoride, loading the catalyst slurry on hydrophilic carbon cloth to obtain an electrocatalytic reduction nitrate electrode, and with the electrocatalytic reduction nitrate electrode as a working electrode, forming a three-electrode system, which is used for electrocatalytic reduction of nitrate in sewage, from the working electrode, a platinum electrode and a saturated calomel electrode. The transition metal carbonate nanomaterial disclosed by the invention is simple in preparation method, high in yield, friendly to environment, low in production cost and high in thermal stability and chemical stability, shows high catalytic activity and selectivity and high cycling stability in electrocatalytic reduction of nitrate, and is suitable for large-scale industrial application.

The invention discloses a hydrothermal method for converting oil or oil hydrolysate, namely, higher fatty acid into long-chain alkane. According to the method, oil or oil hydrolysate, namely, higher fatty acid is taken as a raw material to react in a hydrothermal reactor at 150-450 DEG C for 2-24 h with metal and a metal oxide used as reaction catalysts and metal elements or metal waste used as a reducing agent, and thus, long-chain alkane with the highest yield of 90% can be obtained. The long-chain alkane can be synthesized effectively and highly selectively with the method. Biomass oil or oil hydrolysate, namely, higher fatty acid is used as the raw material, the condition that a large number of fossil fuel is consumed in traditional industrial preparation methods is avoided, preparation of complex noble metal catalysts is not required, and the method is simple to operate and high in conversion rate, requires no pure hydrogen, causes small environmental pollution due to adoption of water as a hydrogen source and a reaction solvent and is beneficial to industrial production. The long-chain alkane product can be used for producing aviation fuel to replace traditional fossil fuel.

The invention discloses a method for producing 4,4-difluoro cyclohexyl formic ether by using counter cyclohexanone formic ether through fluorination, which belongs to the technical field of chemical industry. The method comprises the steps of (1) respectively putting the counter cyclohexanone formic ether, sulfur tetrafluoride and hydrogen fluoride in a pressure kettle, starting blending, enabling the reaction temperature in the pressure kettle to be controlled at 0-80 DEG C, and enabling response time to be 0.5-24.0h; (2) opening an outlet valve of the pressure kettle, enabling surplus gas to be led in alkali liquid, and enabling the gas to be absorbed by the alkali liquid; (3) opening the pressure kettle, enabling reaction liquid to be poured in ice water, starting blending simultaneously, stewing and layering after completing pouring to separate an oil layer; and (4) adjusting the separated oil layer to be neutral by using the alkali liquid, stewing to remove a water layer, adding a desiccating agent to the oil layer, and filtering the desiccating agent to obtain the 4,4-difluoro cyclohexyl formic ether. The method synthesizes target products through a one-step reaction, and is simple in reaction, few in steps, few in secondary products, apt to obtain products with high purity through distillation, high in total recovery, good in safety and favorable for scale production.

The invention provides a method for separating and detecting related substances of 1-butylsulfonyl chloride, which comprises the following steps: dissolving 1-butylsulfonyl chloride in a solvent to prepare a solution, adding an amine reagent into the solution for reaction, and detecting and analyzing by adopting GC. According to the method, all impurities and main components are effectively separated, the specificity, sensitivity, accuracy, precision and the like of the method meet the requirements, and the method can be used for separating and detecting related substances of 1-butylsulfonyl chloride.

The invention discloses application of heat-resisting beta-glucosidase mutants N223T, G224F and N223T / G224F in a conversion of polydatin to resveratrol. According to the heat-resisting beta- glucosidase mutants N223T, G224F and N223T / G224F, the amino acid sequences thereof are represented as SEQ: ID NO. 1, ID NO. 2 and ID NO. 3. By the use of the heat-resisting beta- glucosidase and the mutants thereof to hydrolyze the polydatin for preparing the resveratrol, comparing to the use of wild-type enzymes, the conversion efficiency is high, the reaction byproducts are fewer, the yield and purity are high, the separation and purification are easy, and chemical pollution is less.

The invention discloses application of heat-resistant beta-glucosidase and mutants thereof. The mutants of the heat-resistant beta-glucosidase G224T, N223S and N223S / G224T have the amino acid sequences shown in SEQ: ID NO. 1, SEQ: ID NO. 2 and SEQ: ID NO. 3; the heat-resistant beta-glucosidase and the mutants thereof can be used for preparing quercetin through enzymolysis of quercetin glycoside. The preparation of the quercetin through the heat-resistant beta-glucosidase and the mutants thereof has the advantages of high conversion efficiency, few reaction byproducts, easy separation and purification of the product, less chemical pollution, and high yield and high purity of the obtained quercetin product, and the like.

The invention discloses a hydrothermal method for converting oil or oil hydrolysate, namely, higher fatty acid into long-chain alkane. According to the method, oil or oil hydrolysate, namely, higher fatty acid is taken as a raw material to react in a hydrothermal reactor at 150-450 DEG C for 2-24 h with metal and a metal oxide used as reaction catalysts and metal elements or metal waste used as a reducing agent, and thus, long-chain alkane with the highest yield of 90% can be obtained. The long-chain alkane can be synthesized effectively and highly selectively with the method. Biomass oil or oil hydrolysate, namely, higher fatty acid is used as the raw material, the condition that a large number of fossil fuel is consumed in traditional industrial preparation methods is avoided, preparation of complex noble metal catalysts is not required, and the method is simple to operate and high in conversion rate, requires no pure hydrogen, causes small environmental pollution due to adoption of water as a hydrogen source and a reaction solvent and is beneficial to industrial production. The long-chain alkane product can be used for producing aviation fuel to replace traditional fossil fuel.

The invention relates to a vitamin D3 C-25 site P450 hydroxylase as well as a gene, an expression vector, a strain and application thereof, and belongs to the technical fields of enzyme engineering and gene engineering. The amino acid sequence of the vitamin D3 C-25 site P450 hydroxylase CYP109E1-H is shown as SEQ ID NO.1, the gene cyp109E1-H is inserted into a pMA5 plasmid, bacillus subtilis WB600 is used as an expression host to construct a strain, heterologous expression of the vitamin D3 C-25 site P450 hydroxylase CYP109E1-H is achieved, efficient conversion of the vitamin D3 is successfully achieved, meanwhile, specificity is high, reaction by-products are reduced, production cost is reduced, and the application prospect is wide. And a foundation is laid for further industrial application.

The invention belongs to the technical field of medicine preparation and relates to a preparation method for producing starch-based capsules. The method specifically includes the following steps: starch etherification, gelatinization, defoaming, impurity removal, gel-dipping molding and the like. The hydroxypropyl starch capsule prepared by the invention not only has the advantages of smooth surface, high transparency, short disintegration time limit, etc., but also simplifies the production process, saves production cost, and is suitable for industrial production.

The invention discloses an application of heat-resisting beta-glucosidase mutants N223T, G224F and N223T / G224F to transforming arctiin into arctigenin. Amino acid sequences of the heat-resisting beta-glucosidase mutants N223T, G224F and N223T / G224F are indicated in SEQ: ID NO.1, ID NO.2 and ID NO.3. The arctiin is hydrolyzed to prepare the arctigenin by the aid of heat-resisting beta-glucosidase and the mutants thereof, and as compared with wild-type enzyme, the heat-resisting beta-glucosidase has the advantages of high transformation efficiency, fewer reaction by-products, high yield and purity, easiness in separation and purification, less chemical pollution and the like.

The invention provides a preparation method of oteracil potassium. The method comprises the steps of: dissolving allantoin, alkali and potassium iodide in purified water at room temperature, adding N-halogenated amides in batches after the reaction solution is cooled, and heating up the reaction after the addition is completed. After the reaction is detected, the reaction solution is Lower the temperature, adjust the pH with acid, stir and grow the crystal, and filter with suction. The filter cake is washed with cold purified water until it is colorless, then rinsed with cold anhydrous alcohol solvent, and dried in vacuum to obtain oteracil potassium. Compared with the prior art, the whole process has simple and easy raw materials, easy operation, mild reaction conditions, stable quality, high yield, no pollution to the environment, and is suitable for industrialized production.

The invention discloses a method for preparing eldisine from Catharanthus roseus. The method comprises the following steps: (1) preparation of Catharanthus roseus total alkaloid; (2) preparation of vinblastine sulfate crude product; (3) preparation of vinblastine sulfate pure product; (4) preparation of vinblastine hydrazide; and (5) preparation of eldisine. The method is simple to technique and convenient to operate; and the production keypoints are easy to control, so the method is suitable for industrial production. The silica gel has the advantages of low cost and simple equipment requirements. The vinblastine sulfate refinement process after salification is simple and convenient; and the refined vinblastine sulfate has the advantages of high content and high yield. In the eldisine preparation process, the azidation reaction and hydrolysis reaction are carried out in one reactor, so the reaction time is short, and the amount of reaction byproducts is smaller. After the reaction, the eldisine has the advantages of higher content and high yield.

A preparation method of 2-chloro-N, N-dimethylnicotinamide, the method first disubstituted aminoacrolein and 2-cyano-N, N-dimethylacetamide under the conditions catalyzed by a catalyst Carrying out Knoevenagel reaction, the obtained target intermediate is reacted with hydrogen chloride gas to obtain 2-chloro-N,N-dimethylnicotinamide. The catalyst used in the present invention can greatly improve the yield of the Novenger reaction, the yield can reach more than 97%, and the content can reach more than 97%. The invention has easy-to-obtain raw materials, simple operation, improves the reaction yield, avoids the use of highly toxic reagents such as phosphorus oxychloride and thionyl chloride, has less discharge of three wastes, is environmentally friendly, saves production costs, and is suitable for industrial production.

The invention relates to a cobalt-containing porous material and application thereof to preparation of phenol through benzene hydroxylation. The cobalt-containing porous material is characterized by being prepared by using a method comprising the steps of: dissolving an additive, an inorganic cobalt salt, a surfactant and a silicon source in deionized water according to a certain sequence, stirring and then regulating until a proper pH is reached to obtain sol, ageing under a hydrothermal condition, and then centrifuging, washing, drying and roasting to obtain the cobalt-containing porous material. The cobalt-containing porous material is used as a catalyst, and has high activity and high selectivity in preparation of the phenol through benzene direct hydroxylation.

The invention provides a double-carbonylation preparation method utilizing palladium to catalyze acetylene and relates to the field of preparing succinic acid by an acetylene method. According to thedouble-carbonylation preparation method, acetylene, carbon monoxide and water are utilized as raw materials to be catalyzed and synthesized into succinic acid (anhydride) under the conditions of a reaction temperature as 25 to 75 DEG C and a pressure as 0.1 to 5MPa. To the used catalyst, a palladium compound-lithium halide / hydrogen halide-nitrogenous / phosphine ligand is utilized as the catalyst, wherein n (palladium compound) to n (lithium halide / hydrogen hydride) is equal to 1 to (1 to 10), and n (phosphine / nitrogen ligand) to n (palladium compound) is equal to 1 to (1 to 10). Compared with the prior art, the double-carbonylation preparation method disclosed by the invention has the advantages of moderate reaction conditions, quick reaction speed, high selectivity, simpleness and safety in operation, ability in effectively reducing succinic acid production cost and very good industrial market prospect.