Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biosynthesis method of borneol

A technology of biosynthesis and borneol, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low concentration of borneol in the transformation liquid and no practical application value, and achieve improved selectivity and side effects The effect of less product

Inactive Publication Date: 2010-10-13
GUANGXI UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the borneol concentration of the transformation liquid obtained in these studies is very low, and has no practical application value, so the industrialization research has been a blank all these years.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Use 10g / L peptone, 10g / L glucose, 1.0g / L beef extract, 1.0g / L KH 2 PO 4 , 0.1g / L of MgSO 4 , 1.0g / L of NaCl and 0.5g / L of (NH 4 ) 2 SO 4 Prepare 40 mL of culture medium with pH 5.5, put it into a 250 mL Erlenmeyer flask, then insert the Klebsiella planticola (Klebsiella planticola) bacterial strain preserved on the slant, cultivate it in a shaker at 30°C and 150 rpm for 36 hours, and then place the The cultured bacterial solution was centrifuged at 4°C and 4500rpm for 10 minutes to collect bacterial cells, washed with pH 5.5 phosphate buffer solution twice, and stored in the refrigerator until use.

[0024] Add 20 mL of phosphate buffered saline with pH 6.5, 1 ml of ethanol (4% by volume), the aforementioned bacterial cells and 333 μL of bornyl acetate (85 mM) in sequence in a 250 mL Erlenmeyer flask, and place at 22°C , 2d in a 60rpm shaker. After the reaction, the biocatalyst was separated by filtration and reused, then extracted three times with 10 mL of petrol...

Embodiment 2

[0026] Use 10g / L peptone, 20g / L waste molasses, 5.0g / L beef extract, 1.0g / L KH 2 PO 4 , 0.5g / L MgSO 4 , 0.5g / L of NaCl and 1.0g / L of (NH 4 ) 2 SO 4 Prepare 100 mL of culture medium with pH 7.0, put it into a 1000 mL Erlenmeyer flask, insert Klebsiella planticola (Klebsiella planticola) strains, and culture at 40°C for 40 h in a shaker at 250 rpm. The bacterial solution was centrifuged at 4°C and 4500 rpm for 10 minutes to collect bacterial cells, and the bacterial cells were washed twice with pH 7.0 phosphate buffer solution, and stored in the refrigerator until use.

[0027] In a 500mL Erlenmeyer flask, add 50mL of disodium hydrogen phosphate-citric acid buffer solution at pH 7.0, 5mL of petroleum ether (that is, the volume content is 10%), the aforementioned bacterial cells and 1.5mL of bornyl ester (150mM) , and then placed in a 28°C, 150rpm shaker for 4d. After the reaction is over, the biocatalyst is filtered and separated for reuse, and then the borneol product is ...

Embodiment 3

[0029] With 15g / L corn flour, 15g / L glucose, 5.0g / L beef extract, 0.8g / L KH 2 PO 4 , 0.2g / L MgSO 4 , 1.5g / L of NaCl and 1.0g / L of (NH 4 ) 2 SO 4Prepare 100 mL of culture medium with pH 8.5, inoculate Klebsiella planticola (Klebsiella planticola) strains and culture in a shaker at 45°C and 350rpm for 70h, then place the cultured bacterial solution at 4°C and 4500rpm The bacterial cells were collected by centrifugation for 10 min, and the bacterial cells were washed twice with pH 8.5 phosphate buffer solution, and stored in the refrigerator until use.

[0030] In a 500mL Erlenmeyer flask, add 45mL, pH7.5 phosphate buffered saline, 5mL ethyl acetate (i.e. the volume content is 10%), cultured bacterial cells and 1.5mL bornyl ester (150mM), and then place 35 ° C, 300 rpm shaker reaction 6d. After the reaction is over, the biocatalyst is filtered and separated for repeated use, and then the borneol product is separated by recrystallization, and 150 mg of borneol solid is obtai...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a biosynthesis method of borneol, which comprises the following steps: selecting and separating microbial strains for synthesizing the borneol from the nature, collecting cells after fermentation, taking the cells as a bio-catalyst to be subjected to the hydrolysis reaction of borneol ester in an organic solvent-buffer salt system, and obtaining a borneol product with the borneol content of over 75 percent after separation and refining. The bornrol product is close to the composition of natural borneol, and the method is a novel method for preparing the borneol.

Description

technical field [0001] The invention belongs to the field of biochemical industry and relates to a biosynthesis method of borneol. Background technique [0002] Borneol, also known as borneol, has a chemical name of 1,7,7-trimethylbicyclo[2,2,1]heptan-2-ol. The corresponding stereoisomers will be formed when the hydroxyl group is in different spatial positions, the endo-configuration is orthoborneol, and the exo-configuration is isoborneol. In the perfume and cosmetics industry, orthoborneol has a better fragrance than isoborneol; in medicine, both have antibacterial, analgesic, anti-fertility and other functions, but isoborneol has great side effects. [0003] According to different preparation methods, borneol can be divided into natural borneol and synthetic borneol, and both products are mixtures of orthoborneol and isoborneol. Natural borneol is a processed product of Dipterocarpa dipterocarp resin or the crystallization obtained after sublimation and cooling of trunk...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/02C12R1/22
Inventor 刘幽燕罗苷莉李青云韦藤幼
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com