30results about How to "Detection reached" patented technology

The invention discloses a flow measurement system for detecting tumor cells and an analysis and monitoring method. The system comprises a sample steam formed by enriched cells, a sample stream chamber for storing the sample stream, a sheath tube for storing a sheath fluid, a coherent excitation light beam intersecting with the sample stream, a scattered light receiving objective lens portion for measuring a coherent scattering light beam having a central scattering angle from excited cells, a light splitting and filtering portion, an imaging measurement and data output portion, an image processing circuit, a computer portion and a display portion. The method comprises the following steps of: preparing an enriched cell suspension from a blood or lymph sample, generating coherent scattering light signals, collecting corresponding diffraction image data of different wavelengths and polarizations and transmitting the data into the image storage and processing unit in the computer, extracting image pattern characteristics, determining the positions of the detected cells in the diffraction image characteristic parameter vector sample space, and displaying and outputting the corresponding statistical analysis results. The system and method can rapidly and accurately classify a large amount of cells to achieve the purpose of detecting tumor cells.

The invention relates to the technical field of biochemical detection, and discloses a CuInS / ZnS quantum dot and a method for detecting alkaline phosphatase by using the quantum dot. The CuInS / ZnS quantum dot is taken as a probe in a system of hydrolyzing disodium 4-nitrophenylphosphate by alkaline phosphatase, the fluorescence intensity was measured under the condition of <lambada>ex / <lambada>em=405 / 588nm, and the alkaline phosphatase content is obtained through the change of fluorescence intensity and the linear relationship between the alkaline phosphatase and the fluorescence intensity. The optimal excitation wavelength of the CuInS / ZnS quantum dot prepared by the invention is 405nm, and is overlapped with the maximum substrate absorption peak of the PNP (p-nitrophenol), and an inner filtering effect can be generated, so that the purpose of detecting ALP (alkaline phosphatase) is achieved. The detection sensitivity is high, the anti-interference capability is strong, the sample requirement is low, and the direct detection of a serum sample can be realized.

The detection kit for phytophthora.sojae of the invention belongs to the field of crop disease prevention and treatment and plant quarantine. A pair of specific oligonucleotide primers were designed according to the ITS gene sequence of Phytophthora sojae. The kit includes: 1mL detection solution includes: 0.05mmol Tris.Cl, 0.125mmol KCl, 0.005mmol MgCl 2 , 0.01mmoldNTPs, upstream and downstream primers 0.25mmol, 0.1mgBSA, Taq DNA polymerase 100 units, add ultrapure water to prepare 1mL detection solution. The Phytophthora soybean detection kit based on this primer has strong specificity, sensitivity and stability, and provides a rapid, sensitive and specific technical method for the detection of Phytophthora soybean. The picture shows the specific PCR product of Phytophthora sojae.

The invention discloses a mountain collapse monitoring and early warning device, which comprises a fixed side fixed detection box, and the middle position of the fixed side fixed detection box close to the side of the collapse crack is fixedly connected with an outer fixed detection box through a mechanical telescopic outer tube. The interior of the mechanical telescopic outer tube is provided with a plastic elastic hollow tube, and the fixed side fixed detection box and the outer fixed detection box are both located below the ground, and the fixed side fixed detection box is close to the inner side of the outer fixed detection box The bottom end is fixedly connected with an adjustment control box, and the inside of the adjustment control box is provided with a signal transmitter receiver, a data comparator and a central processing unit. The invention relates to the field of natural geological disaster protection and early warning equipment. The mountain collapse monitoring and early warning device has a high degree of automation and low cost. It is located below the ground and occupies a small space. It can automatically and accurately detect the expansion of the width of mountain cracks, and enhance the timeliness and accuracy of monitoring.

The invention provides a detecting method for a camera. The method comprises the following steps that a square standard image is preset, wherein the standard image comprises an offset detecting graph, a deformation quantity detecting graph and a color detecting graph, the offset detecting graph is arranged in a center position of the standard image and is used for detecting the offset of the camera, the deformation quantity detecting graph is arranged in the standard image, at least comprises one line and one row of graphs, is used for detecting the deformation quantity of the camera and has the preset color for detecting the definition of the camera, the color detecting graph is arranged around the deformation quantity detecting graph in a surrounding way, is used for detecting the color sensing performance of the camera and has the standard color; the standard image is picked up by the camera, and an electronic image of the standard image is obtained; the offset, the deformation quantity, the definition and the color sensing performance of the camera are correspondingly detected through analyzing the offset detecting graph, the deformation quantity detecting graph, the color of the deformation quantity detecting graph and the color detecting graph in the electronic image. The detecting method has the advantage that various performance indexes such as the offset, the deformation quantity, the definition and the color sensing performance of the camera can be detected.

The invention relates to a multi-hydrogen-bond melamine core-shell molecularly imprinted polymer and a preparation method thereof. The preparation method comprises the following steps: by adopting SiO2 as an inner core, performing modification on the surfaces of SiO2 nano particles by using 3-aminopropyltriethoxysilane and glutaraldehyde, and connecting a functional monomer 6-aminouracil; and performing polymerization reaction on melamine as a template molecule, methacrylic acid, an initiating agent and a crosslinking agent to prepare the multi-hydrogen-bond melamine core-shell molecularly imprinted polymer. The multi-hydrogen-bond melamine core-shell molecularly imprinted polymer disclosed by the invention can be used for exchanging the roles of a functional monomer in a conventional three-hydrogen-bond molecularly imprinted system and the template molecule, adopts the functional monomer in the conventional system as the template molecule, adopts a template molecule in the conventional system as the functional monomer in a system disclosed by the invention, has very high selectivity, has specific binding only aiming at melamine and triazine compounds, and has good selective enrichment and adsorption effects in highly-specific food detection.

The invention discloses a voltage detection circuit. The voltage detection circuit comprises a positive detection terminal, a negative detection terminal, a power supply, a switch, a comparator, a voltage stabilizer, a rheostat, a transistor and a display circuit, wherein the positive and negative detection terminals are connected with a positive terminal and a negative terminal of voltage to be detected; a near power supply terminal of the switch is connected with a positive electrode of the power supply; the comparator comprises a normal phase input terminal, an inverting input terminal, a power supply terminal, a grounding terminal and an output terminal; the power supply terminal of the comparator is connected with a far power supply terminal of the switch; the grounding terminal of the comparator is connected with a negative electrode of the power supply; a voltage-stabilizing input terminal of the voltage stabilizer is connected with the far power supply terminal of the switch; a voltage-stabilizing and grounding terminal of the voltage sterilizer is connected with the negative electrode of the power supply; a first fixing terminal of the rheostat is connected with a voltage-stabilizing outer terminal of the voltage stabilizer; a second fixing terminal is connected with the negative electrode of the power supply; a free terminal is connected with the inverting input terminal of the comparator; a grid electrode of the transistor is connected with the output terminal of the comparator; one of a source electrode and a drain electrode of the transistor is connected with the far power supply terminal of the switch; and the display circuit is connected with the other one of the source electrode and the drain electrode of the transistor.

The invention relates to a heating resistance wire fault detection device for a solid heat storage boiler, comprising a body, a universal wheel, a lifting mechanism, a resistance wire detection mechanism, a temperature detection mechanism, a fixing mechanism, a heating resistance wire plate and a cleaning mechanism, the body Universal wheels are installed at the bottom, and a lifting mechanism is installed above the body. A resistance wire detection mechanism is installed on one side of the lifting mechanism, and a temperature detection mechanism is installed below the resistance wire detection mechanism. The fixing mechanism is set on the outer wall of the upper surface of the body. and the heating resistance wire plate is inlaid inside the fixing mechanism, and the cleaning mechanism is arranged inside the body; the invention is provided with a resistance wire detection mechanism, and the balls, the shock absorbing device, the circuit, the wiring wheel, and the heating resistance wire are lowered by the action of the battery. The resistance wire on the board and the slide bar form a circuit, and the motor will not work when the resistance wire on the heating resistance wire board is disconnected, so that the position of the disconnection can be detected well.

The invention provides a nano-gold enhanced highly sensitive detection method for a plurality of lung cancer markers. The detection method comprises the following steps: 1) applying samples of capture antibodies of all the proteins to be detected on an aldehyde group-modified substrate; (2) marking polyclonal or monoclonal detection antibodies of the proteins to be detected on nano-gold; (3) mixing protein chips on which capture antibodies of the proteins to be detected are marked and nano-gold biological composite probes with protein samples to be detected, incubating obtained mixture for a period of time at a temperature of 37 DEG C, and eluting nano-gold probes which have not undergone reaction; 4) adding a gold enhanced reaction solution, observing the solution with naked eyes or microscope, or scanning and taking pictures of the solution with CCD, and determining corresponding protein concentration according to gray values. The method provided in the invention can be applied in a wide variety of fields like clinical diagnosis, monitoring of tumor metastasis, detection of antigens, antibodies and nucleic acids, health quarantine, environment detection, and sensitivity of the method in detecting proteins reaches pg / mL level.

The invention discloses a method for detecting a peer-to-peer (P2P) botnet structure based on network flow clustering. In the method, the detection of the P2P botnet structure is finished by the sequential execution of a real-time communication data acquisition module, a datagram record and filter module, a network flow extraction module, a network flow record and filter module, a network flow clustering module and a data association and result display module. The basic thought of the method is that: a defender determines a command and control relationship between P2P botnet nodes in a monitored network so as to give a P2P botnet structure by identifying a command and control communication in the communication data of the monitored network by utilizing the regularity, namely the characteristics such as the duration, the quantity of datagrams, the quantity of bytes and the like, between the command and the control communication between the P2P botnet nodes. The main innovations are that: communication network flows which have similar characteristics are clustered by a clustering method; and compared with the characteristics in a characteristic set of the command and the control communication, normal communication is distinguished from P2P botnet communication, so that the aim of detecting the P2P botnet structure is fulfilled.

The multiple fluorescence reflecting probe for the fluorescent material detection of sample surface includes one light source to generate exciting light, which irradiates the fluorescent material on the sample to produce fluorescence; two confocal off-axis paraboloidal mirrors to collect and reflect the exciting light and fluorescence incident to the sample; one image detector to detect the fluorescence the fluorescent material in the sample generates; one light filtering lens between the sample and the image detector to filter off the reflected exciting light; and one optical developing lens set located between the light filtering lens and the image detector to focus the fluorescence onto the image detector.

The invention relates to the technical field of electrochemical analysis, in particular to a preparation method and application of an electrochemical sensor based on magnetic nanometer NiFe2O4. Layer NiFe2O4 dispersion liquid, the dosage of NiFe2O4 dispersion liquid is 2-5 μL, which can accurately detect rutin and quercetin at the same time, reduce the process complexity and cost of rutin and quercetin detection; It has good performance and is less affected by common metal ions and 200 times of impurities such as glucose, glycine, citric acid and arginine; and the electrode can also be applied to Simultaneous detection of rutin and quercetin, and the detection method is simple and easy to operate.