40results about How to "Complete elution" patented technology

The invention relates the oral mucosa agent. The invention improves the effects of collection and elution. The one end of hand handle 1 of oral mucosa agent has striking board 2, and the two sides of striking board have ridges 3. Between ridges 3 there are via holes(2-1).

The invention relates to a preparation method for a cinnamaldehyde surface molecularly imprinted polymer. Cinnamic acid and a cinnamic acid polymer with specific recognition characteristic are prepared by adopting a molecular imprinting technology. The molecularly imprinted polymer takes alkylated silica as a carrier, analogue cinnamic acid of cinnamaldehyde as a template, 4-vinylpyridine as a functional monomer and polyethylene glycol dimethacrylate as a cross-linking agent. The obtained molecular imprinting polymer is synthesized into trichloromethane by adopting a thermal polymerization method. The obtained molecular imprinting polymer is used as filler of a solid phase extraction column for self preparing a solid-phase extraction column with the specificity. Meanwhile, the solid-phase extraction column and liquid chromatography are combined and applied to detection of cinnamaldehyde and analogue in an actual cosmetic sample. According to the preparation method disclosed by the invention, high-efficiency separation, enrichment and purification of essence cinnamaldehyde in the cosmetics are realized; the defects of a traditional solid-phase extraction column are overcome; and the obtained solid-phase extraction column can be repeatedly used and is low in cost and favorable in application prospect.

The invention discloses an alcohol-free nucleic acid detection reagent tube, which comprises a main pipe (1), and a plurality of branch pipes (2) are arranged at the lower end of the main pipe (1); the main pipe (1) is sequentially provided with cracking zones ( 3), the first separation plug (4), the cleaning area (5), the second separation plug (6), the branch pipe (2) is provided with the reaction zone (7), and the second separation plug (6) is located in the branch pipe (2) ) and the junction of the main pipe (1), the first separation plug (4) and the second separation plug (6) are provided with a liquid or solid hydrophobic layer (8); the inner wall of the main pipe (1) is provided with There is a magnetic bead channel (9) through to the branch pipe (2); a nucleic acid extraction solution is provided in the lysis zone (3), an alcohol-free cleaning solution is provided in the cleaning zone (5), and an alcohol-free cleaning solution is provided in the reaction zone (7). eluent. The invention can not only reduce the difficulty of operation, but also improve the accuracy and efficiency of detection.

The invention discloses a uranium separation method during gallium treatment in aluminum oxide production through a Bayer process. The uranium separation method comprises the following steps that A, the pH of a special-effect resin acid desorption solution of gallium is controlled to be 1-3; B, a neutralization solution in the step A is made to flow through a pretreated anion exchange resin column, and a column passing solution is separated; C, the column passing solution is neutralized through sodium hydroxide till the pH value reaches 5-9, deposited gallium mud is obtained, the gallium mud is dissolved through alkali liquor, the gallium mud dissolved liquid is obtained, quick lime is added into the dissolved liquid, vanadium is removed through sedimentation, heavy metal in the solution is removed, electrolysis is conducted, and crude gallium products are obtained; D, the anion exchange resin column is subjected to cleaning and impurity removal through diluted acid, and uranium is eluted through acid nitrate or acid chloride; and E, sodium hydroxide is added into the uranium-contained water phase, the pH of the solution is adjusted to be 7-9, sodium diuranate sedimentation is generated, and crude uranium products are obtained after filtering and separation. The uranium separation method is designed according to different characteristics of uranium and gallium, uranium-gallium separation is achieved, and uranium can be recycled.

The present invention relates to the determination of many residues of organophosphorus pesticides in vegetables and fruits, and belongs to the technical field of pesticide content detection, which comprises the following steps: S1: preparation of solution; S2, SPE column solid-phase extraction; S3, gas chromatography measurement: pairing steps The control solution in S1 and the solution after constant volume in step S2 were analyzed by gas chromatography, qualitative by retention time, quantitative by external standard method of peak height, and calculated the residual amount of organophosphorus pesticide in the sample. The detection method provided by the invention has high sensitivity and good reproducibility.

The invention discloses a method for preparing carbonic anhydrase-free hemoglobin, which comprises the following steps: a. fresh anticoagulated bovine blood, washing the blood with 0.9% NaCl, dissolving red blood cells with water, and centrifuging to remove cell fragments and undissolved cells to obtain Supernatant hemoglobin solution to be purified; b, adding filler Na to the hemoglobin solution to be purified in step a 2 SO 4 , to obtain the hemoglobin solution sample to be purified; c, before loading the sample, use the balance solution 0.1-0.3M Na 2 SO 4 / 25-50mM Tris-HCl to equilibrate the medium of affinity chromatography, and then load the hemoglobin solution sample obtained in step b to obtain a flow-through, which is purified hemoglobin. The invention can remove the carbonic anhydrase in the hemoglobin solution, and can have a higher recovery rate, obtain the high-purity carbonic anhydrase while obtaining the high-purity hemoglobin, and kill two birds with one stone, and double benefits.

The invention discloses an optical intelligent silicagel surface molecularly imprinted microsphere and a preparation method thereof. The method comprises the following steps of: arranging a layer of azobenzene derivative molecularly imprinted membrane on the surface of microsphere silicagel through graft polymerization; and grafting the surface of the microsphere silicagel through taking an azobenzene derivative as a functional monomer to obtain a product, adding template molecules and solvents with hydroxyls or carboxyls into the obtained product, and after the obtained mixture is polymerized through an initiator and a crosslinking agent, removing the template molecules through Soxhlet extraction, and drying so as to obtain the final product. Because a microsphere prepared through the graft polymerization in the method disclosed by invention combines the advantages of organic-inorganic materials, the microsphere is good in mechanical strength and chemical stability; and because the template molecules are completely removed, the microsphere is large in handling capacity and uniform in particle size, and can be used as an effective solid-phase extraction material, and therefore, the microsphere has a good application prospect.

The invention discloses a carbon nano tube surface lysozyme molecularly-imprinted polymer. The carbon nano tube surface lysozyme molecularly-imprinted polymer adopts lysozyme as a template molecule, adopts 1-allyl-3-methylimidazolium hexafluorophate ionic liquid and N-tert-butyl acrylamide as a mixing functional monomer, adopts an acrylamide modified carbon nanotube as a matrix, adopts a Tris-HCl buffer solution with the pH being 9 as a reaction system, is obtained under the effect of a cross-linking agent, an initiating agent and a catalyst and has the selective recognition capacity for lysozyme. The number of acting sites is multiple when the template molecule and the functional monomer are combined, the acting force is higher, more effective recognition sites can be formed in the preparation process of the molecularly-imprinted polymer, and therefore the printing effect and the preferential adsorption capacity for lysozyme are better.