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Determination of multiple residues of organophosphorus pesticides in vegetables and fruits

An organophosphorus pesticide and multi-residue technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems affecting consumer food safety, life-threatening organisms, excessive pesticide residues, etc., achieve good extraction effect, improve recovery rate, The effect of enhancing the adsorption effect

Active Publication Date: 2021-12-28
深圳市深大检测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the irrational use of some pesticides has caused pesticide residues in agricultural products to exceed the standard, causing a large amount of pesticide residues in vegetables and fruits, affecting the food safety of consumers
If the detection is not strict, there is a possibility of entering the organism and endangering people's lives

Method used

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  • Determination of multiple residues of organophosphorus pesticides in vegetables and fruits
  • Determination of multiple residues of organophosphorus pesticides in vegetables and fruits
  • Determination of multiple residues of organophosphorus pesticides in vegetables and fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Determination of multiple residues of organophosphorus pesticides in vegetables and fruits, including the following steps:

[0030] S1: Preparation of solution:

[0031] (a1), control solution: Accurately draw 5 mL of methamidophos, dichlorvos, methyl parathion, parathion, malathion, methamidophos, dichlorfos, imidophos, Quetiafos, thionate, monocrotophos, phorate, omethoate standard solution, and diluted with acetone, prepared to a concentration of 0.01μg / mL, 0.05μg / mL, 0.1μg / mL, 0.5μg / mL, 1μg / mL, 2μg / mL, 4μg / mL control solutions.

[0032] (b1), sample solution: burn anhydrous sodium sulfate at 540°C for 2.5h, cool and store in a desiccator for later use; then soak the activated carbon in 2.5mol / L HCl solution, suction filter, and wash with water until there is no chloride ion , and then dried at 120°C for later use;

[0033] Weigh 22g of the sample and pulverize it, add 50g of anhydrous sodium sulfate and grind it into a dry powder, then add 0.6g of activated ca...

Embodiment 2

[0059] 1. Determination of multiple residues of organophosphorus pesticides in vegetables and fruits, including the following steps:

[0060] S1: Preparation of solution: same as Example 1.

[0061] S2: SPE column solid phase extraction:

[0062] (a2), packing and activation: choose C 18 SPE column, weigh 0.2g ACF column, then use 5mL n-hexane: ethyl acetate: acetone 1:5:4 mixed solution, 5mL methanol solution, 3mL methanol water solution with a mass fraction of 10% to activate the SPE column ;

[0063] (b2), rinse: load the sample solution after constant volume in b1, rinse with 2mL distilled water and 5mL methanol solution with a mass fraction of 20% in sequence, and discard the rinse solution;

[0064] (c2), elution: sequentially use 5 mL of petroleum ether, 5 mL of chloroform, 5 mL of isopropanol: ethyl acetate: chloroform in a mixture of 1:2:7.5, 1:4.3:8.5, 1:5.2:9.3 Elution, the elution flow rate is 0.3mL / min, collect the eluate, concentrate to nearly dryness, and di...

Embodiment 3

[0082] 1. Determination of multiple residues of organophosphorus pesticides in vegetables and fruits, including the following steps:

[0083] S1: Preparation of solution: same as Example 1.

[0084] S2: SPE column solid phase extraction:

[0085] (a2), packing and activation: choose C 18 SPE column, weigh 0.2g ACF column, then use 6mL n-hexane: ethyl acetate: acetone 1:6:5 mixed solution, 6mL methanol solution, 6mL methanol aqueous solution with a mass fraction of 10% to pass through the SPE column to activate ;

[0086] (b2), rinsing: load the sample solution after constant volume in b1, rinse with 3mL distilled water and 6mL methanol solution with a mass fraction of 20% in sequence, and discard the eluate;

[0087] (c2), elution: sequentially use 6 mL of petroleum ether, 6 mL of chloroform, 6 mL of isopropanol: ethyl acetate: chloroform in a mixture of 1:3.5:8, 1:4.6:9, 1:5.5:9.5 Elution, the elution flow rate is 0.3mL / min, collect the eluate, concentrate to nearly dryne...

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Abstract

The present invention relates to the determination of many residues of organophosphorus pesticides in vegetables and fruits, and belongs to the technical field of pesticide content detection, which comprises the following steps: S1: preparation of solution; S2, SPE column solid-phase extraction; S3, gas chromatography measurement: pairing steps The control solution in S1 and the solution after constant volume in step S2 were analyzed by gas chromatography, qualitative by retention time, quantitative by external standard method of peak height, and calculated the residual amount of organophosphorus pesticide in the sample. The detection method provided by the invention has high sensitivity and good reproducibility.

Description

technical field [0001] The invention relates to the technical field of pesticide content detection, in particular to the determination of multiple residues of organophosphorus pesticides in vegetables and fruits. Background technique [0002] Pesticide residue refers to the general term for the degradation of the original pesticide and toxic substances remaining in the body of vegetables, grains and other crops after pesticides are used. [0003] The development of modern agriculture has made agricultural products more and more dependent on exogenous substances such as pesticides, antibiotics and hormones. In order to improve the quality of agricultural products and reduce the loss of pests and diseases, organophosphorus pesticides are widely used in the production of fruit trees and vegetables. However, the irrational use of some pesticides has caused pesticide residues in agricultural products to exceed the standard, causing a large amount of pesticide residues in vegetab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/54
CPCG01N30/02G01N30/06G01N30/54G01N2030/047G01N2030/062
Inventor 蒋汶婷
Owner 深圳市深大检测有限公司
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