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Detection method of counterfeiting honey in traditional Chinese medicine honey pill

A detection method and technology of traditional Chinese medicine honey, applied in the directions of measuring devices, instruments, scientific instruments, etc., to achieve the effects of wide range of use, good separation effect and simple preparation method

Inactive Publication Date: 2018-02-06
蒙小翠
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Problems solved by technology

[0003] However, there are serious adulteration phenomena in many honeys on the market today, such as mixing fructose syrup, malt syrup, rice syrup, etc. These syrups contain incompletely hydrolyzed sugars, that is, oligosaccharides above maltopentose. Honey oligosaccharide inspection method can evaluate whether adulterated honey is used as raw material in the production of traditional Chinese medicine honey pills

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  • Detection method of counterfeiting honey in traditional Chinese medicine honey pill
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  • Detection method of counterfeiting honey in traditional Chinese medicine honey pill

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Embodiment 1

[0026] A method for detecting adulterated honey in Chinese medicine honeyed pills, comprising the following steps:

[0027] (1) Preparation of mixed reference substance solution: take appropriate amount of reference substances of maltobiose, maltotriose, maltotetraose, maltopentaose, maltohexaose and maltoheptaose respectively, weigh them accurately, add ethanol solution to prepare the mass concentration Both are 1mg / ml mixed reference substance solution;

[0028] (2) Preparation of the sample solution: Take 2g of the sample, weigh it accurately, put it in a 50ml centrifuge tube, add 20ml of water, vortex until it is completely dissolved; centrifuge at 9000r / min for 15 minutes, take the supernatant and slowly add it to the activated carbon In the column, open the piston, and under the suction of the vacuum pump, let the solution pass through the column, elute with 15ml of 50% ethanol, collect the eluate, put it in a water bath at 65°C and concentrate it to dryness under reduce...

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Abstract

The invention discloses a detection method of counterfeiting honey in a traditional Chinese medicine honey pill. A high performance liquid chromatography method is used for analysis. The method comprises the following detection steps of (1) preparing a mixed reference substance solution; (2) preparing a sample solution; (3) performing chromatography analysis; (4) obtaining the result: in the testsubstance chromatography, the result of chromatography peak occurrence in the corresponding position after the maltopentaose of a mixed reference substance chromatography shows the oligosaccharide inspection, and the result indicates that the honey is counterfeiting. The detection method provided by the invention has the advantages that the high performance liquid chromatography method is used foranalysis; the operation is simple and convenient; convenience and high speed are realized; the detection efficiency is high; the sample solution preparation method is simple; the separation effect isgood; the accuracy is high; good stability and reproducibility are realized; the counterfeiting honey in various kinds of traditional Chinese medicine honey pills can be detected at the same time; the use range is wide.

Description

technical field [0001] The invention relates to the field of detection of traditional Chinese medicines, in particular to a method for detecting adulterated honey in honeyed pills of traditional Chinese medicines. Background technique [0002] Chinese medicine honeyed pill is the most widely used dosage form in Chinese medicine clinic except decoction. After refining, the honey has strong adhesion and can effectively adhere to the drug. After being mixed with the drug powder, the surface of the pill is not easy to harden, disintegrates slowly, and has a long-lasting effect, which is convenient for preserving the drug properties and avoiding the oxidation and deterioration of the active ingredients of the drug. To maintain the inherent odor characteristics of traditional Chinese medicine, honey is often used as a binder clinically. Because honey has the effects of nourishing the lungs and relieving coughs, moistening the intestines and laxatives, nourishing the spleen and ur...

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 蒙小翠邓喜然
Owner 蒙小翠
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