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A method for detecting organophosphorus pesticide multiple residues in vegetables and fruits

An organophosphorus pesticide, multi-residue technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of affecting consumers' food safety, life-threatening organisms, excessive pesticide residues, etc., to achieve less impurity peaks, adsorption and desorption paths short, the effect of improving the recovery rate

Active Publication Date: 2019-10-22
深圳市深大检测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the irrational use of some pesticides has caused pesticide residues in agricultural products to exceed the standard, causing a large amount of pesticide residues in vegetables and fruits, affecting the food safety of consumers
If the detection is not strict, there is a possibility of entering the organism and endangering people's lives

Method used

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  • A method for detecting organophosphorus pesticide multiple residues in vegetables and fruits
  • A method for detecting organophosphorus pesticide multiple residues in vegetables and fruits
  • A method for detecting organophosphorus pesticide multiple residues in vegetables and fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Determination of multiple residues of organophosphorus pesticides in vegetables and fruits, including the following steps:

[0030] S1: Preparation of solution:

[0031] (a1), control solution: Accurately draw 5 mL of methamidophos, dichlorvos, methyl parathion, parathion, malathion, methamidophos, dichlorfos, imidophos, Quetiafos, thionate, monocrotophos, phorate, omethoate standard solution, and diluted with acetone, prepared to a concentration of 0.01μg / mL, 0.05μg / mL, 0.1μg / mL, 0.5μg / mL, 1μg / mL, 2μg / mL, 4μg / mL control solution.

[0032] (b1), sample solution: burn anhydrous sodium sulfate at 540°C for 2.5h, cool and store in a desiccator for later use; then soak the activated carbon in 2.5mol / L HCl solution, suction filter, and wash with water until there is no chloride ion , and then dried at 120°C for later use;

[0033] Weigh 22g of the sample and pulverize it, add 50g of anhydrous sodium sulfate and grind it into a dry powder, then add 0.6g of activated car...

Embodiment 2

[0059] 1. Determination of multiple residues of organophosphorus pesticides in vegetables and fruits, including the following steps:

[0060] S1: Preparation of solution: same as Example 1.

[0061] S2: SPE column solid phase extraction:

[0062] (a2), packing and activation: choose C 18 SPE column, weigh 0.2g ACF column, then use 5mL n-hexane: ethyl acetate: acetone 1:5:4 mixture, 5mL methanol solution, 3mL methanol water solution with a mass fraction of 10% to pass through the SPE column to activate ;

[0063] (b2), rinse: load the sample solution after constant volume in b1, rinse with 2mL distilled water and 5mL methanol solution with a mass fraction of 20% in sequence, and discard the rinse solution;

[0064] (c2), elution: sequentially use 5mL of petroleum ether, 5mL of chloroform, 5mL of isopropanol: ethyl acetate: chloroform in a mixture of 1:2:7.5, 1:4.3:8.5, 1:5.2:9.3 Elution, the elution flow rate is 0.3mL / min, collect the eluate, concentrate to nearly dryness, ...

Embodiment 3

[0082] 1. Determination of multiple residues of organophosphorus pesticides in vegetables and fruits, including the following steps:

[0083] S1: Preparation of solution: same as Example 1.

[0084] S2: SPE column solid phase extraction:

[0085] (a2), packing and activation: choose C 18 SPE column, weigh 0.2g ACF column, then use 6mL n-hexane: ethyl acetate: acetone 1:6:5 mixture, 6mL methanol solution, 6mL methanol water solution with a mass fraction of 10% to activate the SPE column ;

[0086] (b2), rinsing: load the sample solution after constant volume in b1, rinse with 3mL distilled water and 6mL methanol solution with a mass fraction of 20% in sequence, and discard the eluate;

[0087] (c2), Elution: use 6mL of petroleum ether, 6mL of chloroform, 6mL of isopropanol: ethyl acetate: chloroform in a mixture of 1:3.5:8, 1:4.6:9, 1:5.5:9.5 Elution, the elution flow rate is 0.3mL / min, collect the eluate, concentrate to nearly dryness, and dilute to 5mL with acetone;

[0...

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Abstract

The invention relates to detection of organophosphorus pesticide multiple residues in vegetables and fruits and belongs to the technical field of pesticide content detection. The method comprises thefollowing steps: S1) preparation of a solution; S2) SPE column solid-phase extraction; and S3) gas chromatography determination: carrying out gas chromatography on the contrast solution in the S1) andthe constant-volume solution in the step S2), carrying out qualitative analysis based on retention time and quantitative analysis based on a peak height external standard method, and calculating theamount of residues of organophosphorus pesticides in samples. The detection method is high in sensitivity and good in reappearance.

Description

technical field [0001] The invention relates to the technical field of pesticide content detection, in particular to the determination of multiple residues of organophosphorus pesticides in vegetables and fruits. Background technique [0002] Pesticide residue refers to the general term for the degradation of the original pesticide and toxic substances remaining in the body of vegetables, grains and other crops after pesticides are used. [0003] The development of modern agriculture has made agricultural products more and more dependent on exogenous substances such as pesticides, antibiotics and hormones. In order to improve the quality of agricultural products and reduce the loss of pests and diseases, organophosphorus pesticides are widely used in the production of fruit trees and vegetables. However, the irrational use of some pesticides has caused pesticide residues in agricultural products to exceed the standard, causing a large amount of pesticide residues in vegetab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/54
CPCG01N30/02G01N30/06G01N30/54G01N2030/047G01N2030/062
Inventor 蒋汶婷
Owner 深圳市深大检测有限公司
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