30 results about "Nitidine chloride" patented technology

The invention discloses a method for determining the nitidine chloride content in toothpaste by using the double-ternary two-dimensional column high performance liquid chromatography, belonging to the technical field of determining material content by using the high performance liquid chromatography. The method comprises the following steps of: (1) drawing a standard curve chart; (2) placing toothpaste to be tested in a centrifugal tube with a stopper and carrying out constant volume setting on the toothpaste by using methanol; (3) preparing a solution to be tested; and (4) carrying out two-dimensional column separation by using the double-ternary high performance liquid chromatography, determining nitidine chloride by using an ultraviolet detector, and obtaining the content of the nitidine chloride according to the standard curve. The method disclosed by the invention is simple and rapid to operate, good in selectivity, and good in reproducibility; and a chromatographic peak of a target analyte and an impurity chromatographic peak can achieve a better baseline separation effect, and the interference caused by interferents in a toothpaste matrix can be eliminated, therefore, the nitidine chloride in the toothpaste can be subjected to quantitative analysis accurately; and the method has a good separation effect and is wide in linear range.

The invention discloses a method of simultaneously determining content of hesperidin, nitidine chloride and psoralen in eight-tradtional-medicine-herb Longzuan particles through HPLC. The chromatographic conditions adopted in the method are as follow: the specification of the chromatographic column Phenomenex Gemini C18 is (250 mm*4.6 mm, 5 micrometers), the mobile phase is acetonitrile, a 0.1% phosphoric acid water solution is used for gradient elution, the flow velocity is 1 ml / min, the detection wavelength is 286 nm and 245 nm (0-30 min: 286 nm, 30-40 min:245 nm), the column temperature is30 DEG C, and the sample size is 5 micrometers. Under the chromatographic conditions, chromatographic peaks are separated well, good linear relations are formed between hesperidin, nitidine chloride and psoralen and the respective peak areas in the concentration ranges of 84.68-508.1 microgram / milliliter,16.84-101.0 microgram / milliliter and 5.504-33.02 microgram / milliliter (r values are 0.99053, 1.00000 and 0.99998 respectively), the average recovery rates are 98.1%, 99.9% and 100.1% respectively, and the RSD values are 1.3%, 2.7% and 2.5% respectively (n=6). The method is simple, rapid, accurate, high in reproducibility and capable of providing a theoretical basis for comprehensively evaluating and controlling the eight-tradtional-medicine-herb Longzuan particles.

The invention discloses a method for preparing target antineoplastic drug chlorinated nitidine compound, products thereof and an injection containing the products. The method for preparing the target antineoplastic drug chlorinated nitidine compound comprises the steps of pre-treating the chlorinated nitidine to acquire carboxylic acid-modified chlorinated nitidine, then using amphiphilic polymers to implement non-covalent functionalized modification on the outer wall of a single-wall carbon nano tube that is cut to be 20-500 nm long, and coupling the carboxylic acid-modified chlorinated nitidine with the single-wall carbon nano tube after the non-covalent functionalized modification by means of esterification and amidation reactions to acquire the compound. The preparation method of the compound is simple, the solubility of the resulting products can reach 5.0mg / mL, and the antineoplastic target is strong, thus favorably settling the problems of low solubility and strong toxicity of the chlorinated nitidine; in addition the invention can be applied clinically as a substitute for the chlorinated nitidine and provides a new water-soluble compound for the clinical trial of the chlorinated nitidine.

The invention discloses an application of nitidine chloride in resisting osteoporosis and in preventing and treating bone loss diseases caused by osteoclasts; the inventor, through researches, discovers that the nitidine chloride can inhibit the formation of the osteoclasts and a bone absorption function in a dose-dependent mode for the first time; it discovers that the nitidine chloride, by intervening RANKL-activated NF-kB and NFAT signaling pathways, can develop an effect of inhibiting the osteoclasts; and by establishing an ovariectomized osteoporosis mouse model, it further provides the effect of the nitidine chloride on the osteoclasts. The nitidine chloride can be used as an ideal medicine for preventing and treating the bone loss diseases caused by the osteoclasts in the future.

The invention especially relates to a method for separating and preparing dental pulp stem cells, belonging to the field of cell culture techniques. According to the invention, a natural compound nitidine chloride is used as a bacteriostatic agent in the preparation process of dental pulp stem cells, and the bacteriostatic agent has high safety, good stability and obvious bacteriostatic effect, and can effectively reduce bacterial, fungal or mycoplasma infections; so the method is superior to conventional methods simply using antibiotics. In the process of culture, a culture dish is coated with hyaluronic acid and gelatin, thereby increasing the number of wall-adhering dental pulp stem cells and promoting cell growth, so the purpose of effective amplification is achieved. Experimental results show that the advantages of large cell quantities and low contamination rate are obtained when the method provided by the invention is used for amplification of dental pulp mesenchymal stem cells; and the surface markers of the obtained dental pulp stem cells meet standards in detection, positively expressed surface markers are no less than 95%, and negatively expressed surface markers are no more than 2%.

The invention relates to a traditional Chinese medicine extract having poultry and livestock mycoplasma resistant activity. The traditional Chinese medicine extract contains any one or more of the following components: 1-512 mu g / mL of nitidine chloride, 1-512 mu g / mL of ethoxychelerythrine, 1-512 mu g / mL of chelerythrine, 1-512 mu g / mL of sanguinarine, 1-512 mu g / mL of coptisine, 1-512 mu g / mL of palmatine, 1-512 mu g / mL of epiberberine, and 1-512 mu g / mL of glycyrrhetinic acid. The invention further provides a traditional Chinese medicine composition having poultry and livestock mycoplasma resistant activity. Both the traditional Chinese medicine extract and the traditional Chinese medicine composition provided by the invention have the stronger effect for inhibiting poultry and livestock mycoplasma activity in vitro, are safe, green and free from medicine residue, and can be applied to treating and preventing poultry and livestock mycoplasma diseases.

The invention discloses a double ternary two-dimensional column high-performance liquid chromatography method for determining the content of acanthine chloride in toothpaste, and belongs to the technical field of high-performance liquid chromatography for determining substance content. Including the following steps: (1) drawing a standard curve; (2) placing the toothpaste to be tested in a centrifuge tube with a stopper and distilling it to volume with methanol; (3) preparing the solution to be tested; (4) using double ternary high performance liquid chromatography Two-dimensional column separation method, ultraviolet detector to determine the chlorinated chlorinated chlorinated, and the content of chlorinated chlorinated was obtained according to the standard curve. The method of the invention is simple and fast in operation, good in selectivity and good in reproducibility; it can make the chromatographic peak of the target analyte and the impurity chromatographic peak achieve better baseline separation, can eliminate the interference of interfering substances in the toothpaste matrix, and accurately detect the toothpaste Quantitative analysis of dihedral chloride in the sample, and the separation effect is good and the linear range is wide.

The invention discloses a method for purifying nitidine chloride. The method comprises the following steps: after smashing a nitidine medicinal material, reflux-extracting by using acidity alcohol solution, merging acid alcohol extracting solution, regulating the pH value by using acid-base, stewing and filtering; alcohol-precipitating, refrigerating, filtering, and concentrating to obtain the extracting solution as sample solution; uniformly mixing the sample solution and normal phase silica gel or aluminium oxide, drying by distillation, separating and purifying through the normal phase silica gel or aluminium oxide, eluting by using ethyl acetate-absolute ethyl alcohol, recycling a solvent, collecting fractions, dissolving by using methyl alcohol, merging the fractions with the high content through the TLC identification and recrystallizing, to obtain a product as the nitidine chloride; and executing the HPLC assay, wherein the transfer rate is more than 80%, and the crystallization purity is higher than 90%. The method is capable of efficiently separating and purifying the nitidine chloride, and is suitable for the large-scale production. The toxicity is small, few solvents are used, the economic value is high, the environmental pollution is small, and the purity is high.

The invention discloses a preparation method for high-purity nitidine chloride as well as a quality control method of high-purity nitidine chloride. The preparation method is characterized by comprising the following steps of: crushing radix zanthoxyli roots, heating up, refluxing and extracting by using acid alcohol; repeatedly grinding alcohol extract by chloroform; dissolving the obtained precipitates by using methanol, adjusting pH of the solution by using hydrochloric acid, and obtaining nitidine chloride coarse crystals by recrystallization; carrying out recrystallization by using methanol for further improving the purity; preparing by using reverse phase silica gel C-18 and separating by using high-performance liquid chromatography; detecting every part of collected eluant by utilizing an analysis type high performance liquid chromatograph; combining nitidine chloride components with the same retention time, and purity of 90%-98% and more than 98%; concentrating under reduced pressure to obtain nitidine chloride components with purity of 90%-98% and nitidine chloride components with purity bigger than 98%; and carrying out quality control by adopting a thin-layer chromatographic analysis method and a liquid-phase chromatographic analysis method. According to the preparation method disclosed by the invention, not only can the purity of the nitidine chloride be improved, but also the production cost can be reduced.

The invention discloses a synthetic method of nitidine chloride. The synthetic method comprises the following steps: 1) dissolving 3,4-dimethoxybenzoic acid in a first organic solvent, adding thionyl chloride to react, evaporating out the solvent from reactants to obtain an intermediate 1; 2) dissolving the intermediate 1 by use of the first organic solvent, adding 3,4-methylenedioxy naphthylamine, and performing nucleophilic substitution reaction to obtain an intermediate 2; 3) dissolving the intermediate 2 in the first organic solvent, adding boron trifluoride diethyl etherate and di(trifluoroacetoxyl) iodobenzene to react, removing the solvent from the reactants, performing column chromatography on obtained residues on silica gel to obtain an intermediate 3; 4) dissolving the intermediate 3 in a second organic solvent, and performing lithium aluminum hydride reduction, dehydration and dimethyl sulfate methylation under an atmosphere protection condition, and then treating with sodium chloride to obtain the target product nitidine chloride. The synthetic method disclosed by the invention is relatively simple in synthesis route and relatively high in yield of the target product and the yield is higher than 27%.

The invention discloses nitidine chloride, a derivative thereof, and applications of nitidine chloride and the derivative of nitidine chloride in preparing medicines used for preventing and treating dermatosis. Nitidine chloride and the derivative of nitidine chloride comprise nitidine chloride, sanguinarine, and chelerythrine; the medicine can be capsules, tablets, emulsions, cream preparations, gel preparations, or ointments; and weight content of nitidine chloride and the derivative of nitidine chloride ranges from 1 to 99%. Nitidine chloride and the derivative of nitidine chloride are capable of inhibiting human skin abnormal proliferative cell proliferation; effect is excellent; and safety is high.