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Method for determining nitidine chloride content in toothpaste by using double-ternary two-dimensional column high performance liquid chromatography

A kind of chlorinated dimethicone and high-performance liquid chromatography technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of chromatographic column column efficiency, interference in measurement, and difficulty in removal, etc., to achieve the goal of eliminating interference Interference, accurate quantitative analysis, and the effect of increasing the degree of automation

Active Publication Date: 2013-08-07
河北省食品质量监督检验研究院
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Problems solved by technology

The carbon chain of some added components is relatively long, which is difficult to remove in the pretreatment, and at the same time, it also affects the column efficiency of the chromatographic column
Use the traditional high performance liquid chromatography method to separate and detect, and the impurities in it will interfere with the determination of the standard product dihedral chloride

Method used

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  • Method for determining nitidine chloride content in toothpaste by using double-ternary two-dimensional column high performance liquid chromatography
  • Method for determining nitidine chloride content in toothpaste by using double-ternary two-dimensional column high performance liquid chromatography
  • Method for determining nitidine chloride content in toothpaste by using double-ternary two-dimensional column high performance liquid chromatography

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Embodiment 1

[0035] The method for determining the content of acanthine chloride in toothpaste by double ternary two-dimensional column high performance liquid chromatography comprises the following steps: (1) drawing a standard curve of acanthine chloride;

[0036] (2) Accurately weigh 2.0g of the toothpaste to be tested and place it in a 50mL centrifuge tube with stopper, add 5-10mL of methanol to shake, shake well, and dilute to 20mL with methanol;

[0037] (3) Ultrasonicate the stoppered centrifuge tube in step (1) at room temperature for 15 minutes, centrifuge at 10,000 rpm for 5 minutes, take the supernatant and filter it with a 0.22 μm organic phase filter to obtain the solution to be tested;

[0038] (4) Use double ternary high performance liquid chromatography for two-dimensional column separation and an ultraviolet detector to measure the chlorinated acantholine in the solution to be tested. According to the standard curve in step (1), obtain the chlorinated chlorinated acantholin...

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Abstract

The invention discloses a method for determining the nitidine chloride content in toothpaste by using the double-ternary two-dimensional column high performance liquid chromatography, belonging to the technical field of determining material content by using the high performance liquid chromatography. The method comprises the following steps of: (1) drawing a standard curve chart; (2) placing toothpaste to be tested in a centrifugal tube with a stopper and carrying out constant volume setting on the toothpaste by using methanol; (3) preparing a solution to be tested; and (4) carrying out two-dimensional column separation by using the double-ternary high performance liquid chromatography, determining nitidine chloride by using an ultraviolet detector, and obtaining the content of the nitidine chloride according to the standard curve. The method disclosed by the invention is simple and rapid to operate, good in selectivity, and good in reproducibility; and a chromatographic peak of a target analyte and an impurity chromatographic peak can achieve a better baseline separation effect, and the interference caused by interferents in a toothpaste matrix can be eliminated, therefore, the nitidine chloride in the toothpaste can be subjected to quantitative analysis accurately; and the method has a good separation effect and is wide in linear range.

Description

technical field [0001] The invention relates to a method for determining the content of acanthine chloride in toothpaste, and belongs to the technical field of high-performance liquid chromatography for determining the content of substances. Background technique [0002] As food safety incidents have aroused widespread concern in the society, some scholars and public opinion have extended their perspectives to the field of "portable" daily chemical products, and toothpaste, as an oral cleaning product, is a typical representative of this type of product, which is related to personal safety. The quality and safety supervision of functional toothpaste cannot be relaxed. Our country has a glorious and long history of research on the efficacy of Chinese herbal medicines. Chinese herbal medicine toothpaste has a history of decades since the interview, and has become the main product in the domestic toothpaste market. Some international daily chemical companies also want to join t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/14G01N30/46
Inventor 张岩丛斌张敬轩刘敬泽李挥滕文华吕品庞坤李强夏静
Owner 河北省食品质量监督检验研究院
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