51 results about "Linear sweep voltammetry" patented technology

The invention relates to a preparation method of Ni-W-P graded alloy. The preparation method is characterized in that an electrolyte of the Ni-W-P alloy comprises the following components according to concentrations: 20-100 g / L of nickel salt, 10-60 g / L of tungstate, 30-60 g / L of conducting salt, 15-40g / L of a dispersing agent, 10-30 g / L of citric acid, 5-40 g / L of phosphorous acid and 10-30 g / L of boric acid; the preparation method comprises the following steps: immersing workpieces after pretreatment in the electrolyte of 45-75 DEG C, adopting the linear sweep voltammetry or cyclic linear sweep voltammetry for scanning at the speed of 0.1-100 mV / s within the range from minus 1.3 V to minus 0.5 V, and adjusting the sedimentary dynamic states of different ions by adjusting the deposition potential, so as to obtain the Ni-W-P graded alloy. According to the preparation method of the Ni-W-P graded alloy, the Ni-W-P graded alloy coating prepared by adopting the preparation method has a single-layer or multi-layer graded structure and contains amorphous phases, the microhardness can reach 500-1150 Hv, the corrosion resistance and wear resistance of the coating are obviously improved, and the preparation method has a potential application prospect.

The invention provides an electrochemical detection method for catalytic silver deposit of reducing type oxidized graphene, as well as applications thereof. The method comprises the following steps: modifying a DNA probe, which can be identified specifically by a to-be-detected object, on a gold electrode, to obtain a working electrode; reacting the working electrode, the to-be-detected object with known specific activity, and standard sample solution of substances capable of cutting the DNA probe, which can be identified specifically by a to-be-detected object, into oligonucleotides single chains, loading the reacted electrode with reducing type oxidized graphene, performing silver deposit reaction together with silver ion-containing solution, dissolving out silver monomer deposited on the working electrode, determining the dissolution volt-ampere current value by utilizing a linear sweep voltammetry, and drawing a standard cure; according to the standard cure, calculating to obtain the specific activity of the to-be-detected sample. The established electrochemical detection method has the advantages of being labeling-free, safe for detection process, simple to operate, easy to miniature, wide to apply and the like.

This case involves a detection method for microRNA in the liquid to be tested based on a constant temperature reaction: immerse the electrode in the first DNA fragment molecule solution, react at 37°C for 6 hours, then immerse in the mercaptohexanol solution for 1 hour; then immerse in the silver nanoparticle solution for reaction 1h, then immerse in the solution of the second DNA fragment molecule and react for 0.5h; prepare microRNA standard solution; then immerse in microRNA standard solution, add the third DNA fragment molecule, Klenow fragment and dNTPs, and react at 37°C for 1h; then immerse in Nt. React in BbvCI solution for 1 h; use linear sweep voltammetry to detect and draw a standard curve; obtain the electrical signal of the test solution according to the above method, and compare it with the standard curve to obtain the concentration of microRNA in the test solution. This case has the advantages of simple operation, high sensitivity, strong specificity, low background signal, and wide application range.