The invention provides an electrochemical detection method for catalytic silver deposit of reducing type oxidized graphene, as well as applications thereof. The method comprises the following steps: modifying a DNA probe, which can be identified specifically by a to-be-detected object, on a gold electrode, to obtain a working electrode; reacting the working electrode, the to-be-detected object with known specific activity, and standard sample solution of substances capable of cutting the DNA probe, which can be identified specifically by a to-be-detected object, into oligonucleotides single chains, loading the reacted electrode with reducing type oxidized graphene, performing silver deposit reaction together with silver ion-containing solution, dissolving out silver monomer deposited on the working electrode, determining the dissolution volt-ampere current value by utilizing a linear sweep voltammetry, and drawing a standard cure; according to the standard cure, calculating to obtain the specific activity of the to-be-detected sample. The established electrochemical detection method has the advantages of being labeling-free, safe for detection process, simple to operate, easy to miniature, wide to apply and the like.