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Method for detecting cholesterol by synergistically catalyzing sliver deposition through enzyme and graphene

A cholesterol and graphene technology, applied in the measurement device, material electrochemical variables, material analysis by electromagnetic means, etc., can solve the problems of expensive instruments, time-consuming technical requirements, complicated operation, etc., to achieve low background interference and ensure stability. , the effect of large specific surface area

Active Publication Date: 2018-02-06
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

The instruments used in these methods are expensive, complicated to operate, time-consuming and technically demanding. It is necessary to establish a rapid, sensitive and easy-to-operate cholesterol detection method.

Method used

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  • Method for detecting cholesterol by synergistically catalyzing sliver deposition through enzyme and graphene
  • Method for detecting cholesterol by synergistically catalyzing sliver deposition through enzyme and graphene
  • Method for detecting cholesterol by synergistically catalyzing sliver deposition through enzyme and graphene

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Embodiment Construction

[0038] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0039] A method for the detection of cholesterol by synergistically catalyzing silver deposition with enzyme and graphene, the detection principle see figure 1 .

[0040] The implementation steps are as follows:

[0041] 1. Electrode pretreatment: Before using the screen-printed electrode, first place it in 0.5 mol / L H 2 SO 4In the solution, use the cyclic voltammetry scanning method to scan, the scanning voltage is -0.2 V ~ 1.0 V, and scan 10 times within this range; after the scanning, the electrode is washed with clean water and dried for later use;

[0042] 2. Electrode modification and enzyme immobilization: Put the activated screen-printed electrode into 5 mL of 0.01% HAuCl 4 In this method, the deposition was performed for 120 s while stirring with a magnetic stirrer, and the deposition potential was -0.5 V. After the constant pote...

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Abstract

The invention discloses a method for detecting cholesterol by synergistically catalyzing sliver deposition through an enzyme and graphene. According to the method, nanogold is deposited on the surfaceof a screen-printed electrode by adopting an electrodeposition technology, graphene, CHER (Cholesterol Esterase) and CHOD (Cholesterol Oxidase) dripping onto the surface of the electrode are adsorbedonto the surface of the screen-printed electrode under the action of electrostatic adsorption, the cholesterol is hydrolyzed under the actions of the CHER and CHOD to generate H2O2, and the H2O2 andthe graphene have reducibility, so that synergistically catalysis of the sliver deposition is realized; Ag<+> is reduced into Ag and deposited on the surface of the screen-printed electrode. Anodic dissolution current of Ag is detected through a linear sweep voltammetry (LSV), and a relation curve between the current and cholesterol concentration is described to realize detection of the cholesterol. The method has the advantages of easiness in operation, saving in time, low cost and lower detection line.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a method for detecting cholesterol. Background technique [0002] Cholesterol is an indispensable and important substance in mammals. The normal cholesterol level in human serum ranges from 200 mg / dL to 239 mg / dL. The detection methods mainly include colorimetry, gravimetric method, fluorescence method, thermal determination method, molecular luminescence method, enzymatic method, gas chromatography, high performance liquid chromatography, electrochemical method, dynamic spectroscopy and temperature measurement method. The experimental costs of these traditional detection methods are relatively expensive. Sandro C. et al. constructed a highly sensitive cholesterol biosensor based on carbon nanotubes and cytochrome P450scc modified screen-printed electrodes in 2008 (Carrara S, ShumyantsevaV V, Archakov A I, et al. Screen-printed electrodes based on carbon nanotubes and cytoch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/308G01N27/3277
Inventor 黄勇曾俊翔崔丽杰李桂银梁晋涛董辰杨王志宏周治德
Owner GUILIN UNIV OF ELECTRONIC TECH
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