34 results about "Immobilized cellulase" patented technology

The invention provides a method for improving enzymatic activity and utilization rate of immobilized enzymes in a constant magnetic filed three-phase fluidized bed. The method can effectively improve the enzymatic activity and the utilization rate of magnetic immobilized cellulolytic enzymes by applying a constant magnetic field to a magnetic field three-phase fluidized bed and regulating and controlling various parameters of the constant magnetic field, such as magnetic field strength, magnetic field action time, gas velocity, fluid velocity and the like. The magnetic immobilized cellulolytic enzymes used by the method of the invention have excellent stability and can be repeatedly used for 9 times after being recycled, and the recovery ratio of the enzymatic activity is over 42 percent. The method can improve the enzymatic activity and the utilization rate of magnetic immobilized cellulolytic enzymes so as to effectively reduce cost and simultaneously can improve the degradation rate of macromolecular substances and provides the basis for realizing continuous production.

The invention belongs to the technical field of oil extraction, and in particular relates to a method for extracting wheat germ oil by immobilized enzyme method. The method comprises the following steps: pretreatment of enzyme immobilization carrier carbon fiber and raw material wheat germ, crushing of wheat germs and then sieving with a 40 to 100 mesh screen, adjustment of material-liquid ratio and solution ionic strength, use of carbon fiber carrier immobilized cellulase and amylase for enzymolysis treatment of the raw material, centrifugal separation of enzymolysis liquid of wheat enzymolysis to obtain the wheat germ oil, and detection of oil extraction rate. Through use of carbon fiber immobilized bienzyme and water enzyme method for extraction of the wheat germ oil, nutrients in the raw material are not destroyed, the obtained wheat germ oil is clear and transparent, and has good quality, and the oil extraction rate is effectively improved.

The invention provides a novel oil extraction method which combines enzymolysis with a solvent and aims at overcoming the defects that lixiviation time of a lixiviation method is relatively long, the dosage of enzyme of an aqueous enzymatic method is high, and the enzyme cannot be used repeatedly. The novel oil extraction method comprises the steps that cellulase and alkali protease are immobilized under an optimum immobilization condition, and an enzyme membrane is taken out to be wound on the surface of a cylinder, so as to obtain an immobilized cellulase roll and an immobilized alkali protease roll; then the moisture of germ flakes is adjusted by adjusting the humidity of the air under a vacuum condition, and the optimum relative humidity is 75%; the immobilized cellulase roll is used to roll the germ flakes continuously for 30min to allow the cellulase to perform the enzymolysis on plant cell walls; and then the immobilized alkali protease roll is used to roll the germ flakes continuously for 30min for separating protein from oil. After the enzymolysis of the germ flakes is finished, the solvent is used to extract the oil, the extraction time is only required to be 35min, and the residual oil reaches below 0.5%. In addition, an immobilized enzyme column can act on the material germ continuously for more than 24h, and the enzymic activity is still kept at 80%.

The invention belongs to the technical field of immobilized enzymes, relates to a soluble-insoluble UCST type PMAAc vector as well as an immobilized enzyme and an application thereof, and in particular to a preparation method of the soluble-insoluble high critical solution temperature (UCST) type sodium polymethylacrylate (PMAAc) vector, an application of the immobilized enzyme and an applicationof an immobilized enzyme degraded cellulose material thereof. The preparation method comprises the following steps: dissolving acrylic acid and ammonium persulfate through de-ionized water; slowly dropping methacrylamide which is dissolved by de-ionized water; uniformly shaking an obtained solution, filling nitrogen until bubbling and transferring an obtained solution into a single-mouth flask; implementing vacuumizing in nitrogen protection; conducting a reaction under magnetic stirring; implementing centrifuging after conducting cooling; discarding supernatant liquid; and heating up a resuspended material via de-ionized water, so that the finished product (the PMAAc vector) is obtained. The vector provided by the invention is applicable to immobilized cellulase, and the vector is used for degrading cellulose materials; the immobilized enzyme prepared by the invention is excellent in storage stability and reuse effect; and efficiency of hydrolyzing and catalyzing the cellulose materials is also obviously improved.

The invention relates to the field of materials and discloses a preparation method of a mesoporous material. The preparation method comprises the following steps: adding an acid or alkali solution into a precursor in a stirring state, performing hydrolysis to obtain a transparent solution, cooling the transparent solution and recycling ethyl alcohol; adding a template solution into the transparent solution in a stirring state, and adjusting the pH value of the mixed solution to be neutral by an acid or alkali solution for gelatinizing; smashing wet glue obtained by the gelatinizing; removing the template through water washing; carrying out suction filtration on the mixed solution after water washing to obtain white gel; and drying the gel, smashing, and sieving, thus obtaining the mesoporous material. According to the preparation method of the mesoporous material provided by the invention, an immobilized enzyme can be obtained by adding bioactive substances including enzymes into a reaction system in the preparation process. The experiment shows that the enzyme activity of the immobilized enzyme of the mesoporous material nearly keeps unchanged after the immobilized enzyme is repeatedly used.

The invention provides a method for immobilizing cellulase and hydrolyzing cellulose. The method for preparing immobilized cellulase uses sodium alginate as a carrier, and utilizes OH in sodium alginate ‑ and COOH ‑ Oxygen atom and Ca 2+ The principle of ion exchange reaction forms a gel, polyethylene glycol is used as a porogen and a hydrophilic agent, glutaraldehyde is used as a crosslinking agent, and the gel is formed between chitosan and sodium alginate-polyethylene glycol immobilized enzyme The polyelectrolyte membrane improves the stability of the immobilized enzyme. Using immobilized cellulase to hydrolyze cellulose can realize the repeated utilization of cellulase. Adding polyethylene glycol and chitosan can improve the enzyme fixation rate and enzyme activity of immobilized cellulase, reduce the Michaelis constant, increase the pore density of the carrier surface, increase the reducing sugar content of the enzymatic hydrolysis reaction, the enzymatic hydrolysis yield and TS degradation rate. The preparation and application method of the invention is simple and convenient to operate, mild in fixing conditions, low in investment and friendly to the environment.