Preparation method of sustained-release immobilized cellulase for feeding

Abstract

The invention discloses a preparation method of sustained-release immobilized cellulase for feeding, which belongs to the field of cellulase preparation. The method comprises the following steps: taking gelatin, mixing the gelatin with deionized water, a glucose aqueous solution and a polyethylene glycol aqueous solution, and mixing the obtained mixture with glycerin, a glacial acetic acid solution and a crosslinking agent so as to obtain a sustained-release coating material for standby application; and taking and mixing mannitol, tryptone, yeast and agar, adjusting the pH value of the obtained mixture so as to obtain a clostridium perfringens culture medium, inoculating the clostridium perfringens culture medium, inoculating clostridium perfringens in a rapeseed bran cake so as to obtain a rapeseed bran cake-glucose solution, after the clostridium perfringens are subjected to sealed culture, opening the culture medium for feeding air, evaporating clostridium perfringens containing rapeseed bran cake particles until the particles are dry, mixing the dry particles with the standby sustained-release coating material, pressing the obtained mixture in a die, and drying the obtained product, so that the sustained-release immobilized cellulase is obtained. The method disclosed by the invention has the beneficial effects that the preparation cost is reduced by 18% in comparison with traditional methods, the production efficiency is high, the enzyme activity of the cellulase is increased by 42%, and the enzymatic activity is greater than 9250 IU / g.

Description

technical field [0001] The invention relates to a preparation method of sustained-release immobilized cellulase for feed, and belongs to the field of cellulase preparation. Background technique [0002] Cellulase (β-1,4-glucan-4-glucan hydrolase) is a general term for a group of enzymes that degrade cellulose to generate glucose. It is not a monomeric enzyme, but a synergistic multi-component The enzyme system is a complex enzyme, mainly composed of exo-β-glucanase, endo-β-glucanase, and β-glucosidase, as well as a highly active xylanase, which acts on Cellulose and products derived from cellulose. Microbial cellulase is of great significance in converting insoluble cellulose into glucose and destroying cell walls in fruit and vegetable juices to improve juice yield. Cellulase is widely found in organisms in nature. Bacteria, fungi, and animals can produce cellulase. The production of bacterial cellulase is less, mainly endoglucanase, most of which have no degradative act...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention: for the production of cellulase by solid fermentation method, it occupies a large area, is easy to be contaminated with bacteria, and has poor production stability, and the technology is not easy to master, resulting in low enzyme activity, low yield, and high cost. In view of the disadvantages of poor controllability, a preparation method of sustained-release immobilized cellulase for feed is provided. The present invention mixes gelatin with deionized water, glucose aqueous solution and polyethylene glycol aqueous solution, and then mixes it with glycerin, glacial acetic acid solution, The cross-linking agent is mixed into a slow-release coating material for subsequent use; then mannitol, tryptone, yeast and agar are mixed to adjust the pH value to prepare a culture medium for Clostridium perfringens, which is inoculated with Clostridium perfringens for cultivation. Clostridium perfringens is inoculated in the vegetable bran cake glucose solution prepared from vegetable bran cake. After sealed culture, it is opened to let in air, evaporated to dryness, mixed with the spare slow-release coating material in a mold, pressed, and dried. have to