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Process for transforming geniposide under enzymatic action of polyurethane foam immobilized cellulase

A technology of polyurethane foam and cellulase, which is applied in the directions of enzymes, enzymes, etc., which are fixed on/in organic carriers, can solve the problems of low utilization rate and waste of cellulase, and achieve easy expansion of industrialization and low cost. , the effect of simple equipment

Inactive Publication Date: 2019-03-08
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to improve the defects of low cellulase utilization rate and serious waste, the present invention provides a polyurethane foam immobilized cellulase enzyme-catalyzed geniposide conversion process, in the case of ensuring enzyme activity, increasing the immobilized The repeated use of the enzyme greatly improves the conversion efficiency of cellulase to geniposide

Method used

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  • Process for transforming geniposide under enzymatic action of polyurethane foam immobilized cellulase
  • Process for transforming geniposide under enzymatic action of polyurethane foam immobilized cellulase
  • Process for transforming geniposide under enzymatic action of polyurethane foam immobilized cellulase

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Effect test

example 1

[0022] Put the powdery cellulase that mass fraction is 30.0% into the polyurethane hair of 1.3% deionized water, 37.5% polyethylene glycol 400, 0.5% Contonin DC-193, 0.6% dibutyltin dilaurate The foam material is mixed evenly, after adding 30% diphenylmethane diisocyanate, stir in a water bath at 20°C under the mechanical stirring condition of 500r / min for 20s, and then cure at room temperature for 24h, that is, the polyurethane foam can be fixed on the carrier. cellulase. Place the prepared immobilized cellulase directly in the aqueous solution of geniposide under the conditions of 35°C and pH=6, carry out the enzymatic reaction for 70 minutes, take out the immobilized cellulase, and make the After the enzyme is inactivated, the membrane is filtered and the concentration of the generated genipin solution is measured by high performance liquid chromatography, and the conversion rate of geniposide is calculated. As a result, the cumulative conversion rate of geniposide of the ...

example 2

[0024] Put the mass fraction of 27.6% cellulase into 2.1% deionized water, 35.0% polyethylene glycol 600, 0.7% Tween 80, 0.4% dibutyltin dilaurate in the polyurethane foaming material and mix Evenly, after adding 35.0% diphenylmethane diisocyanate, stir in a water bath at 20°C with a mechanical stirring speed of 500r / min for 20s, and then cure at room temperature for 24h, that is, the immobilized cellulose with polyurethane foam as the carrier enzyme. Place the prepared immobilized cellulase directly in the aqueous solution of geniposide under the conditions of 15°C and pH=3, carry out the enzymatic reaction for 70 minutes, take out the immobilized cellulase, and make the After the enzyme is inactivated, the membrane is filtered and the concentration of the generated genipin solution is measured by high performance liquid chromatography, and the conversion rate of geniposide is calculated. As a result, after 7 cycles of use, the cumulative conversion rate of geniposide of the...

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Abstract

The invention provides a process for transforming geniposide under an enzymatic action of polyurethane foam immobilized cellulase. By using unit perforated polyurethane foam as a carrier of the immobilized cellulase, the process for transforming the geniposide under the enzymatic action of the polyurethane foam immobilized cellulase is obtained, so that the cellulase is fixed in a pore unit of thepolyurethane foam in the embedded form; by optimizing a foaming material and the usage of the foaming material, the opening percentage and aperture size are controlled to control the contact area between the cellulase and a substrate; and therefore, the cellulase repeated use frequency and use efficiency are enhanced in the case of guaranteeing a certain geniposide transformation rate. It is shown by an experimental result that the geniposide cumulative transformation rate of the polyurethane foam immobilized cellulase is 413.3% of equivalent-quality free cellulase after the polyurethane foamimmobilized cellulase is used for seven times in circulation; and moreover, selectable ranges of an enzyme and an glucoside suitable for the process are very large; according to the differences between the glucosides needing to be transformed, different invertases are selected to be immobilized. In addition, the process for transforming the geniposide under the enzymatic action of the polyurethane foam immobilized cellulase, provided by the invention, has the advantages that adopted instrument equipment is simple, the operation is simple and convenient, the preparation cost is low and the industrialization is easy to expand.

Description

technical field [0001] The present invention relates to a polyurethane foam immobilized cellulase enzyme-catalyzed geniposide conversion process, in particular to a novel polyurethane foam with cells opened as a carrier, immobilized cellulase by an embedding method and A new process for converting glycosides. Background technique [0002] Geniposide (geniposide) is one of the main components of gardenia, belonging to iridoid glycosides. Modern pharmacological studies have shown that geniposide has various pharmacological effects such as anti-inflammation, anti-rheumatoid arthritis, anti-Alzheimer's disease, anti-diabetes and neuroprotection. The hydrolyzed product of geniposide, genipin, has pharmacological activities such as hepatoprotection, antihypertensive, and antitumor, and is often used as a natural cross-linking agent. [0003] Cellulase (β-1,4-glucan-4-glucan hydrolase) is a complex enzyme mainly composed of exo-β-glucanase, endo-β-glucanase and β- Glucosidase an...

Claims

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Application Information

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IPC IPC(8): C12P19/60C12N11/08C12N9/42
CPCC12N9/2437C12N11/08C12P19/60
Inventor 赵修华王璐刘佩岩邓怡平吴微微王力张晓雪
Owner NORTHEAST FORESTRY UNIVERSITY
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