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Method for improving enzymatic activity and utilization rate of immobilized enzymes in constant magnetic filed three-phase fluidized bed

A technology of immobilized enzyme activity and three-phase fluidized bed, applied in the direction of immobilized enzymes, biochemical equipment and methods, enzymes, etc., can solve the difficulties of enzyme recovery, low enzyme activity of immobilized enzymes, and low enzyme reuse rate and other problems, to achieve the effect of improving enzyme activity and utilization rate, good stability, and improving enzyme activity and utilization rate

Inactive Publication Date: 2010-04-21
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the deficiencies and defects of low immobilized enzyme enzyme activity, low enzyme reutilization rate and difficult enzyme recovery in the existing fluidized bed enzyme reactor, the purpose of the present invention is to provide a three-phase fluidized bed with constant magnetic field. Method for Improving Enzyme Activity and Utilization Rate of Immobilized Enzyme

Method used

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  • Method for improving enzymatic activity and utilization rate of immobilized enzymes in constant magnetic filed three-phase fluidized bed
  • Method for improving enzymatic activity and utilization rate of immobilized enzymes in constant magnetic filed three-phase fluidized bed
  • Method for improving enzymatic activity and utilization rate of immobilized enzymes in constant magnetic filed three-phase fluidized bed

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Embodiment 1

[0041] (1) preparation of chitosan acetic acid solution

[0042] Be 0.1% acetic acid solution and chitosan solution (chitosan molecular weight is 100,000) preparation 30mg / mL chitosan acetic acid solution with volume percentage, use Na 2HPO 4 Adjust the pH value to 4.0-5.0 to obtain chitosan acetic acid solution;

[0043] (2) Preparation of magnetic chitosan microspheres

[0044] Add 200mL chitosan acetic acid solution in 1000mL flask, then add 3.5g Fe 3 o 4 Powder, stir and mix evenly, add 10mL Span-80, 300mL paraffin, after being dispersed evenly, add 5.0mL glutaraldehyde at 40°C, stir for 1 hour, then add 5.0mL glutaraldehyde dropwise, and continue the reaction for 3 hours Finally, the magnetic microspheres are obtained, let stand to cool to room temperature, wash the magnetic microspheres with petroleum ether and absolute ethanol in sequence, collect the magnetic microspheres with a magnetic field, dry them in an oven at 60°C, and sieve to obtain dry magnetic chitosan ...

Embodiment 2

[0051] (1) preparation of chitosan acetic acid solution

[0052] Prepare the chitosan acetic acid solution of 50mg / mL with the acetic acid solution of volume percent concentration 1% and chitosan solution (chitosan molecular weight is 100,000), use Na 2 HPO 4 Adjust the pH to 4.0-5.0 to obtain chitosan acetic acid solution.

[0053] (2) Preparation of magnetic chitosan microspheres

[0054] In a 1000mL flask, add 300mL chitosan acetic acid solution, then add 5.0g Fe 3 o 4 Powder, stir and mix evenly, add 20mL Span-80, 400mL paraffin, after uniform dispersion, add 10.0mL glutaraldehyde at 40°C, stir for 1 hour, then raise the temperature to 60°C and add 10.0mL glutaraldehyde dropwise , after continuing to react for 4 hours, the magnetic microspheres were obtained, left to cool to room temperature, washed the magnetic microspheres with petroleum ether and absolute ethanol in sequence, collected the magnetic microspheres with a magnetic field, dried in an oven at 65°C, and si...

Embodiment 3

[0060] (1) preparation of chitosan acetic acid solution

[0061] Configure the chitosan acetic acid solution of 40mg / mL with the acetic acid solution and the chitosan solution (chitosan molecular weight is 100,000) of volume percent concentration 0.5%, use Na 2 HPO 4 Adjust the pH to 4.0-5.0 to obtain chitosan acetic acid solution.

[0062] (2) Preparation of magnetic chitosan microspheres

[0063] In a 1000mL flask, add 300mL chitosan acetic acid solution, then add 4.0g Fe 3 o 4 Powder, stir and mix evenly, add 15mL Span-80, 350mL paraffin, after being dispersed evenly, add 8.0mL glutaraldehyde at 40°C, stir for 1 hour, then add 5.0mL glutaraldehyde dropwise, and continue the reaction for 4 hours Finally, the magnetic microspheres are obtained, let stand to cool to room temperature, wash the magnetic microspheres with petroleum ether and absolute ethanol in sequence, collect the magnetic microspheres with a magnetic field, dry them in an oven at 60°C, and sieve to obtain ...

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Abstract

The invention provides a method for improving enzymatic activity and utilization rate of immobilized enzymes in a constant magnetic filed three-phase fluidized bed. The method can effectively improve the enzymatic activity and the utilization rate of magnetic immobilized cellulolytic enzymes by applying a constant magnetic field to a magnetic field three-phase fluidized bed and regulating and controlling various parameters of the constant magnetic field, such as magnetic field strength, magnetic field action time, gas velocity, fluid velocity and the like. The magnetic immobilized cellulolytic enzymes used by the method of the invention have excellent stability and can be repeatedly used for 9 times after being recycled, and the recovery ratio of the enzymatic activity is over 42 percent. The method can improve the enzymatic activity and the utilization rate of magnetic immobilized cellulolytic enzymes so as to effectively reduce cost and simultaneously can improve the degradation rate of macromolecular substances and provides the basis for realizing continuous production.

Description

technical field [0001] The invention relates to a method for improving the enzyme activity and utilization rate of magnetically immobilized enzymes, in particular to a method for improving the enzyme activity and utilization rate of immobilized enzymes in a constant magnetic field three-phase fluidized bed. Background technique [0002] At present, the degradation methods of macromolecular high-viscosity substances (such as natural chitosan macromolecular systems) mainly include: chemical methods, physical methods, and enzymatic hydrolysis methods. Because enzymatic degradation has the advantages of specificity, selectivity, easy control, and basically no pollution to the environment, enzymatic hydrolysis is an ideal method. [0003] In immobilized enzyme engineering, small particles are often used as immobilized enzyme carriers because of their high immobilized specific surface area. However, for the common immobilized enzyme reactor—fixed bed reactor, the small particle c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12N11/00
Inventor 李冰寇灵梅李琳郭祀远陈玲李晓玺
Owner SOUTH CHINA UNIV OF TECH
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