Method for preparing immobilized cellulase
A technology of cellulase and cellulose acetate, which is applied in the direction of fixing on/in the organic carrier, and can solve the problems of poor stability, easy inactivation, denaturation, and easy inactivation of free cellulase
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specific Embodiment approach 1
[0008] Embodiment 1: The method for immobilizing cellulase is realized through the following steps: 1. Preparation of composite membrane: 5 g of cellulose acetate is dissolved in 100 mL of acetone. The prepared polypropylene membrane was soaked in acetone solution dissolved in cellulose acetate to form a film naturally, and the cellulose acetate / polypropylene composite membrane was rinsed with deionized water and dried several times for later use; 2. Enzyme immobilization: the enzyme solution Cellulase with a concentration of 0.5-2.5% and disodium hydrogen phosphate-potassium dihydrogen phosphate buffer are mixed at a pH of 2 to 6, and a cellulose acetate / polypropylene composite film is added. After stirring for 1~5h, add 0.35~0.55% glutaraldehyde for cross-linking reaction for 1~5h, take out the enzyme film and wrap it on the surface of the cylinder to make an immobilized cellulase roll; Activity: The activity of cellulase is 5.103 U / cm 2 , the immobilized cellulase roller c...
specific Embodiment approach 2
[0009] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 2, the concentration of glutaraldehyde is selected to be 0.45-0.55%. Other steps are the same as in the first embodiment.
specific Embodiment approach 3
[0010] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 2, the concentration of glutaraldehyde is selected to be 0.45-0.55%, and the immobilization temperature is 40-50°C. Other steps are the same as in the first embodiment.
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