Method for extracting wheat germ oil by immobilized enzyme method
A technology of wheat germ oil and immobilized enzymes, which is applied in the direction of fat oil/fat production and fat production, and can solve problems such as low oil yield, waste of resources, and toxic solvents
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Embodiment 1
[0023] Example 1: The 6 k carbon fibers were chopped into 0.5 cm lengths, and then pretreated: soaked in acetone for 6 h, filtered to recover the supernatant, washed with 4 times the volume of carbon fiber distilled water, and then kept the volume of distilled water greater than 2 times the volume of carbon fibers, and in Treat in a boiling water bath for 3 hours, take out the carbon fiber, rinse it with distilled water three times the volume of the carbon fiber, and put it in a bellows for drying. Then add 10 g / L into a 250 mL shake flask containing 50 mL deionized water, shake at 180 rpm for 6 h, and finally take out the pelleted carbon fibers and dry them for later use.
[0024] Put 50 g of raw wheat germ into the microwave turntable according to the material thickness of 1 cm, and then heat it in the microwave oven for 90 s, then take out the wheat germ, and grind the treated wheat germ into fine particles. After the wheat germ is ground, it is sieved with a 40-mesh stan...
Embodiment
[0028] Example 2: Compared with Example 1, the difference of this example is that the 3 k carbon fibers are chopped into lengths of 1.5 cm, and then pretreated: after soaking in acetone for 6 h, put 3 times the volume of deionized water into a boiling water bath for treatment After 4 h, take out the carbon fiber, rinse it with distilled water twice the volume of the carbon fiber, and put it in a bellows to dry for later use.
[0029] Put 50 g of raw wheat germ into the turntable of the microwave oven according to the material thickness of 1 cm, and then heat it in the microwave oven for 100 s, then take out the wheat germ, and grind the treated wheat germ into fine particles. After the wheat germ is ground, it is sieved with a 60-mesh standard sieve to obtain smaller granular wheat germ. Then use the vegetable oil extraction solvent as the extraction reagent, add pH=4.8, 0.05 mol / L citrate buffer according to the ratio of solid to liquid of 2:5 and stir to obtain the slurry....
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