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Nano superfine fiber membrane of polyvinyl alcohol with immovable cellulase

A technology of polyvinyl alcohol and cellulase, which is applied in the direction of immobilization on/in the organic carrier, can solve problems such as no research reports, and achieve the effect of simple preparation process and high biological enzyme activity

Inactive Publication Date: 2005-03-30
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Using PVA as a raw material, nanometer ultrafine fibers with an average diameter of 200nm can be obtained by electrospinning, which can be used as carriers for immobilized enzymes, especially hydrolytic enzymes such as cellulase. No research reports or invention patents have been seen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Take 0.6g of polyvinyl alcohol (the degree of polymerization is 1750±50, the degree of alcoholysis is 98%), and dissolve it in 9mL of acetic acid buffer solution (pH=4.6) under magnetic stirring and heating; take 0.03g of cellulase , Dissolved in 1 mL of acetic acid buffer solution (pH = 4.6). Then, the two were mixed to form 10 mL of a clear spinning solution. Inject the spinning solution into a 20 mL syringe with a flat needle with an inner diameter of 0.8 mm, fix the syringe on a micro-syringe pump, and place a grounded metal receiving screen vertically at a distance of 10 cm from the needle. Connect the needle to a 10kV high-voltage DC power supply, set the flow rate to 0.2mL / h for electrospinning, and collect a film with an average diameter of 200nm on the receiving screen and a thickness of 25μm after 12 to 14 hours. Contains a small amount of fusiform beads. Remove the microfiber membrane from the receiving screen, place it above the container of 25% glutaralde...

Embodiment 2

[0013] Take 0.7g of polyvinyl alcohol (the degree of polymerization is 1750±50, the degree of alcoholysis is 98%), and dissolve it in 8mL of acetic acid buffer solution (pH=4.6) under magnetic stirring and heating; take 0.02g of cellulase , Dissolved in 1 mL of acetic acid buffer solution (pH = 4.6). Then, the two were mixed to form 10 mL of a clear spinning solution. Inject the spinning solution into a 20 mL syringe with a flat needle with an inner diameter of 0.8 mm, fix the syringe on a micro-syringe pump, and place a grounded metal receiving screen vertically at a distance of 10 cm from the needle. Connect the needle to a 10kV high-voltage DC power supply, set the flow rate to 0.2mL / h for electrospinning, and collect ultrafine fiber membranes with a diameter of about 200nm on the receiving screen after 12 to 14 hours. The ultrafine fibers contain a small amount of Fusiform beads. Remove the microfiber membrane from the receiving screen, place it above the container of 25...

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PUM

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Abstract

This invention relates to fixed type cellulase polyvinyl alcohol nanometer super fine fibrous membrane prepn. method. It is composed of (by wt.%): 80-99 polyvinyl alcohol and 1-20 cellulase to form the membrane which thickness is 10-50 num with average 200 nm fiber. The procedures are as follows: polyvinyl alcohol with molecular wt. 60 thousands-100 thousands is dissolved in acetic acid buffer solution to form 5-8% solution, then proceeding static textile method to form a membrane, then proceeding valeric dialdehyde vapor crosslinking to produce the product. Said fixed type cellulase membrane material is used for degradation of chitin, with high biological activity of enzyme and it can be used for several times. Its production process is simple.

Description

technical field [0001] The invention relates to a polyvinyl alcohol nano-ultrafine fiber film for immobilizing cellulase and a preparation method thereof, which belongs to the enzyme immobilization technology. Background technique [0002] Electrospinning is a spinning process in which polymer solution or melt forms ultrafine fibers by means of high-voltage electrostatic action. Ultrafine fibers with a diameter of 3nm-1 μm can be prepared by electrospinning, and can be directly collected into ultrafine fiber membranes (Zheng M, Zhang Y Z, Kotaki M, Ramakrishna S.A review on polymernanofibers by electrospinning and their applications in nanocomposites. CompositesScience and Technology, 2003, 63, 2223-2253). Due to the characteristics of large specific surface area and high porosity, electrospun ultrafine fibers are used as carriers for immobilized enzymes, which is conducive to full contact between enzymes and substrates and reduces steric hindrance, so it has broad developm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04
Inventor 袁晓燕邬丽丽张春雪盛京
Owner TIANJIN UNIV
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