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Immobilized cellulose and a method for hydrolyzing plant material thereby for extracting active ingredients

A technology of cellulase and plant materials, which is applied in the direction of fixing on/in organic carriers, chemical industry, organic chemistry, etc., can solve the problems of non-uniform contact and poor effect, so as to overcome insufficient contact and save costs Effect

Inactive Publication Date: 2009-10-07
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plant material is a solid material insoluble in water, so the pretreatment with immobilized enzyme is a heterogeneous reaction, that is, the immobilized enzyme cannot be in uniform contact with the treated plant material, and the effect of enzyme pretreatment is worse than that of solution enzyme.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Preparation of immobilized cellulase

[0041] (1) Using glutaraldehyde as a cross-linking agent, immobilized enzyme: 0.5g N-succinyl chitosan, dissolved in distilled water, under magnetic stirring conditions, add 0.6ml of 25% mass fraction of glutaraldehyde, and then add 0.025g cellulase, magnetic stirring at room temperature for 2h;

[0042] (2) Add 20ml of pH3 glycine-hydrochloric acid buffer solution, the N-succinyl chitosan-immobilized cellulase is precipitated in a gel state, filtered, and the gel is collected. Then wash repeatedly at least 6 times with 20ml pH3 glycine-hydrochloric acid buffer until there is no free cellulase activity in the washing liquid. Add 20ml of citric acid-sodium citrate buffer solution at pH 8, stir magnetically, and dissolve the gel to prepare a solution of cellulase immobilized by N-succinyl chitosan, which is stored at 4°C for later use.

Embodiment 2

[0043] Example 2: Preparation of immobilized cellulase

[0044] (1) Take glutaraldehyde as cross-linking agent, immobilized enzyme: 0.5gN-succinyl chitosan, be dissolved in distilled water, under magnetic stirring condition, add the glutaraldehyde of 0.1~2ml 25% mass fraction, then Add 0.005~0.1g cellulase, and stir magnetically at room temperature for 1~2h;

[0045] Step (2) is as step (2) in Embodiment 1.

Embodiment 3

[0046] Example 3: Extract flavonoids from Ginkgo biloba.

[0047] Ginkgo biloba leaves were collected in Suzhou in October, dried and ground into powder, passed through a 60-mesh sieve, and decolorized with ether; N-succinyl chitosan-immobilized cellulase was N-succinyl chitosan-immobilized cellulose obtained in Example 1 enzyme solution.

[0048] Experimental group: N-succinyl chitosan immobilized cellulase to treat flavonoids extracted from Ginkgo biloba leaves.

[0049] (1) Weigh 5g of powder, add 30ml of pH4 citric acid-sodium citrate buffer, then add 60ml of N-succinyl chitosan immobilized cellulase solution, and stir magnetically for 2 hours at 45°C to treat the plant The cell wall of the material is subjected to enzymatic treatment.

[0050] (2) Adjusting the pH value to 3 to precipitate and separate the immobilized enzyme in a gel state.

[0051] (3) Then add 210ml of ethanol to the suspension system of Ginkgo biloba powder, raise the temperature to 70°C, keep it fo...

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PUM

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Abstract

The invention discloses an immobilized cellulose and a method for hydrolyzing plant material thereby for extracting active ingredients. The carrier of the immobilized cellulose is N-succinyl-chitosan, and the cellulose is selected from Trichoderma viride, aspergillus niger or fusarium cellulose; in the process of using the immobilized cellulose for hydrolyzing plant material, the immobilized cellulose can be dissolved in the solution or separated out in gel form by controlling the pH value of the solution, thus not only leading the immobilized cellulose to fully contact with the plant material and further improving the hydrolysis efficiency of the immobilized cellulose, but also faciliating the separation and recycling of the immobilized cellulose.

Description

technical field [0001] The invention relates to immobilized cellulase and a method for extracting active components by hydrolyzing plant materials, in particular to a method for extracting active components by hydrolyzing plant materials with immobilized cellulase. Background technique [0002] Plant material is a rich treasure trove of natural active ingredients, containing flavonoids and other physiologically active compounds. [0003] The active ingredients in plant materials exist in cells, and plant cells are composed of cell walls and protoplasts. The cell walls are dense structures composed of cellulose, hemicellulose, pectin, lignin and other substances. From plant materials, such as Extracting various compounds or mixtures from leaves, flowers, and roots of plants is one of the ways to obtain certain compounds. [0004] At present, the methods for extracting active ingredients from plant materials include water extraction, ethanol extraction, supercritical fluid ex...

Claims

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Application Information

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IPC IPC(8): C12N11/10C07G99/00C07C69/732
CPCY02P20/50
Inventor 周建芹
Owner SUZHOU UNIV
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