Method for preparing low molecular weight chitosan under steady magnetic field condition

A technology of oligochitosan and chitosan, which is applied in the direction of fixing on or in inorganic carriers, fixing on/in organic carriers, fermentation, etc., which can solve the difficulty of enzyme recovery and low solid mass transfer coefficient and other issues to achieve the effect of reducing cost, increasing degradation rate and improving utilization rate

Inactive Publication Date: 2009-04-01
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the existing fluidized bed liquid-solid mass transfer coefficient low, the deficiency of difficult enzyme recovery, the object of the present invention is to provide a kind of utilizing magnetic field three-phase (gas, liquid, solid) fluidized bed enzymolysis reactor (see invention Name "magnetic field three-phase fluidized bed enzymatic hydrolysis reactor", patent number 200720061568.x) to degrade chitosan substances with large molecular weight to prepare oligomeric chitosan (molecular weight <10000)

Method used

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  • Method for preparing low molecular weight chitosan under steady magnetic field condition
  • Method for preparing low molecular weight chitosan under steady magnetic field condition
  • Method for preparing low molecular weight chitosan under steady magnetic field condition

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Embodiment 1

[0026] The method for preparing chitosan oligosaccharides under steady and constant magnetic field conditions comprises the steps:

[0027] The first step, configure the chitosan acetic acid solution of 1mg / mL with the acetic acid solution of volume percent concentration 1% and chitosan solution (chitosan molecular weight is 60,000), adjust pH to be 4.0 with NaOH solution, obtain chitosan Acetic acid solution.

[0028] The second step, the preparation of magnetic chitosan microspheres

[0029] Get 100mL chitosan acetic acid solution and place in a 500mL flask, add 2.0g Fe 3 o 4 Powder, stir and mix evenly, add 4mL Span-80, 8mL n-butanol, 100mL paraffin, after being uniformly dispersed, add 2mL formaldehyde dropwise at 40°C, after 10min, add 4mL formaldehyde dropwise, after 1h, heat up to 80°C , then add 6mL of glutaraldehyde dropwise, continue to stir and react for 1h. After the reaction is over, the magnetic microspheres are obtained and cooled to room temperature; wash th...

Embodiment 2

[0034] The method for preparing chitosan oligosaccharides under steady and constant magnetic field conditions comprises the steps:

[0035] The first step, configure the chitosan acetic acid solution of 10mg / mL with the acetic acid solution of volume percent concentration 2% and chitosan solution (chitosan molecular weight is 60,000), adjust pH to be 4.5 with NaOH solution, obtain chitosan Acetic acid solution.

[0036] The second step, the preparation of magnetic chitosan microspheres

[0037] Get 150mL chitosan acetic acid solution and place in 500mL flask, add 3.0g Fe 3 o 4Powder, stir and mix evenly, add 6mL Span-80, 10mL n-butanol, 150mL paraffin, after being uniformly dispersed, add 6.0mL formaldehyde dropwise at 60°C, after 130min, add 6.0mL formaldehyde dropwise, after 2h, heat up to At 60°C, add 8.0 mL of glutaraldehyde dropwise, continue stirring for 2 hours, and when the reaction is over, magnetic microspheres are obtained, which are left to cool to room temperat...

Embodiment 3

[0042] The method for preparing chitosan oligosaccharides under steady and constant magnetic field conditions comprises the steps:

[0043] The first step, configure the chitosan acetic acid solution of 30mg / mL with the acetic acid solution and chitosan solution (chitosan molecular weight 60,000) of concentration 3% by volume percentage, adjust pH to be 5.0 with KOH, obtain chitosan acetic acid solution .

[0044] The second step, the preparation of magnetic chitosan microspheres

[0045] Get 200mL chitosan acetic acid solution and place in 500mL flask, add 3.5g Fe 3 o 4 Powder, stir and mix evenly, add 10mL Span-80, 30mL n-butanol, 300mL paraffin, after being uniformly dispersed, add 8.0mL formaldehyde dropwise at 80°C, after 60min, add 8.0mL formaldehyde dropwise, after 3h, heat up to 100°C, add 10.0mL glutaraldehyde dropwise, continue to stir and react for 3h, the reaction is over, to obtain magnetic microspheres, let stand and cool to room temperature; wash the magnetic...

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Abstract

The invention discloses a method for preparing low-molecular chitosan under a static magnetic field, which comprises the steps of: the preparation of chitosan acetic acid solution, the preparation of magnetic chitosan microspheres, the preparation of magnetic immobilized cellulose and the preparation of the low-molecular chitosan. The method not only can improve the degradation rate of macromolecular substances, but also can provide theoretical base for realizing continuous production; simultaneously, the method is convenient for recycling the enzyme, improving the utility ratio of immobilized enzyme and reducing the cost.

Description

technical field [0001] The invention relates to a method for preparing oligomeric chitosan, in particular to a preparation method for degrading high-molecular-weight chitosan into small-molecular-weight chitosan in a three-phase fluidized bed enzymolysis reactor with a steady magnetic field generated by direct current. Background technique [0002] Due to its unique and superior physiological activity and functional properties, chitosan oligosaccharides can activate and proliferate bifidobacteria in the human intestinal tract, improve the phagocytosis of macrophages, promote the growth of spleen antibodies, and inhibit the growth of tumor cells ; reduce blood pressure, blood sugar, blood lipids, adsorb cholesterol; have strong antibacterial and antibacterial effects and significant moisturizing and moisture absorption capabilities in the microenvironment, so the development and application in the fields of food health care and biomedicine have huge market potential. The futu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12N11/14C12N11/10
Inventor 李冰寇灵梅郭祀远李琳陈玲刘国琴李晓玺
Owner SOUTH CHINA UNIV OF TECH
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