30 results about "Co immobilization" patented technology

The invention discloses a method for co-immobilization of nitrite bacteria-denitrifying bacteria, belonging to a method for co-immobilization of cells. The method comprises the following steps: (1) embedding the denitrifying bacteria by use of sodium alginate and calcium chloride first; (2) mixing immobilized globules prepared in step (1) with the nitrite bacteria and sodium alginate, and dripping into calcium chloride for second embedding; (3) inoculating the co-immobilized nitrite bacteria-denitrifying bacteria prepared in step (2) in a culture medium containing ammonia nitrogen for culturing, wherein the co-immobilized nitrite bacteria-denitrifying bacteria can be used for degradation of the ammonia nitrogen. The method has the advantages that 1, co-immobilization culture of the nitrite bacteria and the denitrifying bacteria in the same carrier is realized for the first time; 2, the method for preparing the co-immobilized globules is simple and convenient, the operation procedures are easy to control, and the prepared co-immobilized globules are easy to store and reuse; 3, due to the co-immobilization culture of the nitrite bacteria and the denitrifying bacteria, the co-immobilized nitrite bacteria-denitrifying bacteria have higher denitrification efficiency than the traditional strain which is not immobilized or immobilized simplex strain, and is easy to be separated from the water body and recyclable.

The invention relates to a reversible double enzyme co-immobilization method capable of regulating and controlling enzyme ratio. By the method, various kinds of enzyme are fixed on the surfaces of multifunctional magnetic nanoparticles modified by dopamine and derivative thereof through a DNA mediated immobilization technology, the method is mild in synthesis condition, and the immobilization quantity of different enzyme in a multi-enzyme reaction system can be controlled by adjusting the number of the functional groups on the surfaces of the magnetic nanoparticles. The immobilized multi-enzyme system established by the method has the advantages of high activity, high stability and the like.

The invention belongs to the field of wastewater treatment, discloses a preparation method and application of co-immobilization mycelium pellets with the function of removing various pollutants, and relates to a preparation method and application of co-immobilization mycelium pellets. The problems that an existing mycelium pellet degradation function is single and the degradation effect is poor are solved. The method comprises the steps of preparing an aspergillus fumigatus spore suspension and a bacillus cereus suspension; 2, taking the aspergillus fumigatus spore suspension and the bacillus cereus suspension, inoculating the aspergillus fumigatus spore suspension and the bacillus cereus suspension into a mycelium pellet pelletizing culture medium, performing shake cultivation, and obtaining the compound mycelium pellets. The co-immobilization mycelium pellets are used for degrading lignin, cellulose, hemicellulose, dyes and heavy metal ions. The compound mycelium pellets can treat paper waste, can also be used for treating dye wastewater and heavy metal ion wastewater, and has the good treatment effect in a system with high alkaline and high temperature.

The invention specifically relates to a co-immobilization method for DNA polymerase and reverse transcriptase, belonging to the field of enzyme engineering. The method comprises the following steps: adding sodium alginate into a first buffer, then adding DNA polymerase, carrying out mixing and immobilizing, and then carrying out vacuum drying so as to obtain immobilized DNA polymerase; adding a second buffer and reverse transcriptase into the immobilized DNA polymerase, carrying out mixing and immobilizing, then carrying out vacuum drying and then carrying out washing with the second buffer; and then carrying out lyophilization so as to obtain the co-immobilized DNA polymerase and reverse transcriptase. According to the invention, DNA polymerase and reverse transcriptase are co-immobilizedon a same carrier, so one-step conversion of a RT-PCR reaction is realized, and the catalytic properties of DNA polymerase and reverse transcriptase are integrated to eliminate certain interference factors, and thus, the overall catalytic efficiency of DNA polymerase and reverse transcriptase to RT-PCR reactions is improved.

The invention discloses an amino-acid oxidase supported catalyst used for oxidizing amino acid to prepare ketonic acid and relates to the technical field of biological enzyme catalysts. A preparation method of the amino-acid oxidase supported catalyst includes the steps of firstly, preparing a co-immobilization agent; secondly, preparing a compound carrier; thirdly, preparing an activating carrier; fourthly, preparing the amino-acid oxidase supported catalyst. The prepared amino-acid oxidase supported catalyst can evidently increase the yield of the ketonic acid converted from the amino acid, the yield of an ketonic acid intermediate prepared from cephalosporin C is increased to above 95%, and the purity of the ketonic acid intermediate reaches above 99%.

The invention discloses a method and apparatus for immobilizing cells to treat wastewater wherein abandoned mycelium is used as a carrying agent for co-immobilization of white-rot fungi and bacteria, the enzyme system in the bacterium group is employed for coupled catalytic degradation of heterocyclic compounds and phenol in the waste water at the presence of oxygen supply. The device includes a reactor with moving sieving board and an oxygen supplying device.

The invention discloses co-immobilization glucose oxidase / catalase microspheres and application thereof in preparation of gluconic acid (salt) for catalytic oxidation of glucose. The co-immobilization glucose oxidase / the catalase microspheres can effectively promote covalent linkage of carriers and enzyme molecules, immobilization efficiency is improved greatly, service life of immobilization glucose oxidase (GOD) / catalase (CAT) is prolonged remarkably, the co-immobilization glucose oxidase / the catalase microspheres can be used repeatedly, are low in production cost, and facilitate sustainable development. Gluconic acid (salt) prepared by the co-immobilization glucose oxidase / the catalase microspheres is high in yield, reaction condition is temperate, a device is simple and easy to obtain, and the production method is environment-friendly.

The invention discloses a method for co-immobilization of nitrite bacteria-denitrifying bacteria, belonging to a method for co-immobilization of cells. The method comprises the following steps: (1) embedding the denitrifying bacteria by use of sodium alginate and calcium chloride first; (2) mixing immobilized globules prepared in step (1) with the nitrite bacteria and sodium alginate, and dripping into calcium chloride for second embedding; (3) inoculating the co-immobilized nitrite bacteria-denitrifying bacteria prepared in step (2) in a culture medium containing ammonia nitrogen for culturing, wherein the co-immobilized nitrite bacteria-denitrifying bacteria can be used for degradation of the ammonia nitrogen. The method has the advantages that 1, co-immobilization culture of the nitrite bacteria and the denitrifying bacteria in the same carrier is realized for the first time; 2, the method for preparing the co-immobilized globules is simple and convenient, the operation procedures are easy to control, and the prepared co-immobilized globules are easy to store and reuse; 3, due to the co-immobilization culture of the nitrite bacteria and the denitrifying bacteria, the co-immobilized nitrite bacteria-denitrifying bacteria have higher denitrification efficiency than the traditional strain which is not immobilized or immobilized simplex strain, and is easy to be separated from the water body and recyclable.

The invention discloses a microwave-assisted co-immobilization method of aldehyde and ketone reductase and glucose dehydrogenase. The method comprises: mixing and activating a carrier cross-linking agent, centrifuging, washing the precipitate with PBS solution, centrifuging, and redispersing the precipitate In PBS solution; add aldehyde ketone reductase and glucose dehydrogenase to the dispersion liquid, irradiate the mixed solution at 0-10°C, 20-45W microwave conditions for 1-5min, centrifuge, take the precipitate and wash it with PBS solution to obtain a total Immobilized enzyme; compared with free enzyme, the co-immobilized enzyme prepared by the present invention has improved catalytic activity, thermal stability, and pH stability; glucose dehydrogenase as its coenzyme regeneration system can provide NAD required for the reaction (P)H reduces costs and is conducive to industrial production.

The invention discloses a noncellular synthetic biology based preparation method of 2-phenylethanol and an application. According to the preparation method and the application, three recombinases including ARO8, ARO10 and ADH1 are utilized to constitute a co-reaction system, and a complicated metabolic process of an Ehrlich pathway requiring multi-step continuous reaction and multi-enzyme participation for synthesis of 2-phenylethanol in microorganisms is simplified to be performed in the same reaction system in vitro, so that the environmental pollution is reduced and the reaction process is shortened. Furthermore, the three recombinases are subjected to carrier-free co-immobilization with glutaraldehyde serving as a cross-linking agent, Combi-CLEAs (Combi-Cross-linked Enzyme Aggregates) are prepared, a target product is synthesized in vitro, accordingly, the enzymes are further recycled on the basis of the beneficial effects, and the production cost is reduced; the preparation method fills up the technical blank of multi-enzyme preparation of 2-phenylethanol in vitro, and important reference is provided for biological preparation of 2-phenylethanol.