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Co-immobilization glucose oxidase/catalase microspheres and application thereof in production of gluconic acid or gluconic salt

A technology of glucose oxidase and catalase, which is applied in the fields of immobilization on/in organic carriers, chemical industry, sustainable manufacturing/processing, etc., can solve the problems of high immobilization cost, low recovery rate of enzyme activity, process Complicated problems, to achieve the effect of improving immobilization efficiency, high yield, and simple equipment

Inactive Publication Date: 2014-11-05
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The main purpose of the present invention is to provide a co-immobilized glucose oxidase / catalase (GOD / CAT) microsphere that overcomes the disadvantages of high immobilization cost, complicated process, and low recovery rate of enzyme activity in the past. The microspheres have the advantages of high recovery rate of enzyme activity, stable immobilized enzyme, and reusability.

Method used

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  • Co-immobilization glucose oxidase/catalase microspheres and application thereof in production of gluconic acid or gluconic salt

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Experimental program
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Effect test

Embodiment 1

[0026] A method for preparing co-immobilized glucose oxidase / catalase microspheres, the specific method steps are as follows:

[0027] Use 2% (v / v) bovine serum albumin solution as the amination reagent, the average pore size of the carrier is 100-200nm, according to the mass of the carrier: the volume of the amination reagent is 1:40, and the carrier is dispersed in the 2% (v / v) amination reagent , at pH 8.0, 200 rpm shaking reaction at 60° C. for 2 h, and the aminated carrier was collected. GOD and CAT were dissolved in 0.5 mol / l phosphate buffer at pH 6 according to the ratio of GOD:CAT activity ratio of 1:2. Disperse the aminated carrier in the enzyme solution, react at 30°C for 2 hours at a stirring speed of 200 rpm, add 2 times the volume of the reaction solution of acetone and 0.5% (v / v) glutaraldehyde to the reaction solution, and continue the reaction for 1 hour. The above reaction solution was filtered, the filtrate was discarded, and the solid matter was collected....

Embodiment 2

[0029] Use 2% (v / v) ethylenediamine solution as the amination reagent, the average pore size of the carrier is 100-200nm, according to the mass of the carrier: the volume of the amination reagent is 1:40, and the carrier is dispersed in the 2% (v / v) amination reagent , at pH 8.0, 200 rpm shaking reaction at 60° C. for 2 h, and the aminated carrier was collected. GOD and CAT were dissolved in 0.1 mol / l phosphate buffer at pH 6 according to the ratio of GOD:CAT activity ratio of 1:2. Disperse the aminated carrier in the enzyme solution, react at 30°C for 2 hours at a stirring speed of 200 rpm, add 2 times the volume of the reaction solution of acetone and 0.5% (v / v) glutaraldehyde to the reaction solution, and continue the reaction for 1 hour. The above reaction solution was filtered, the filtrate was discarded, and the solid matter was collected. For the collected solid matter, it was washed 3 times with 0.01 mol / l phosphate buffer solution of pH 7.0, and dried at 4°C to prepar...

Embodiment 3

[0031] Use 2% (v / v) ethylenediamine solution as the amination reagent, the average pore size of the carrier is 400-500nm, according to the mass of the carrier: the volume of the amination reagent is 1:40, and the carrier is dispersed in the 2% (v / v) amination reagent , at pH 8.0, 200 rpm shaking reaction at 60° C. for 2 h, and the aminated carrier was collected. GOD and CAT were dissolved in 0.1 mol / l phosphate buffer at pH 6 according to the ratio of GOD:CAT activity ratio of 1:2. Disperse the aminated carrier in the enzyme solution, react at 30°C for 2 hours at a stirring speed of 200 rpm, add 2 times the volume of the reaction solution of acetone and 0.5% (v / v) glutaraldehyde to the reaction solution, and continue the reaction for 1 hour. The above reaction solution was filtered, the filtrate was discarded, and the solid matter was collected. For the collected solid matter, it was washed 3 times with 0.01 mol / l phosphate buffer solution of pH 7.0, and dried at 4°C to prepar...

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Abstract

The invention discloses co-immobilization glucose oxidase / catalase microspheres and application thereof in preparation of gluconic acid (salt) for catalytic oxidation of glucose. The co-immobilization glucose oxidase / the catalase microspheres can effectively promote covalent linkage of carriers and enzyme molecules, immobilization efficiency is improved greatly, service life of immobilization glucose oxidase (GOD) / catalase (CAT) is prolonged remarkably, the co-immobilization glucose oxidase / the catalase microspheres can be used repeatedly, are low in production cost, and facilitate sustainable development. Gluconic acid (salt) prepared by the co-immobilization glucose oxidase / the catalase microspheres is high in yield, reaction condition is temperate, a device is simple and easy to obtain, and the production method is environment-friendly.

Description

technical field [0001] The invention relates to a production method of gluconic acid or its salts, in particular co-immobilized glucose oxidase / catalase microspheres and their application in catalyzing the oxidation of glucose to produce gluconic acid or its salts. Background technique [0002] Gluconic acid is a product of glucose oxidation. After derivatization, a series of gluconic acid products can be obtained, including calcium gluconate, potassium gluconate, sodium gluconate, magnesium gluconate, zinc gluconate, ferrous gluconate, manganese gluconate, gluconic acid Copper, barium gluconate and gluconolactone and other products. The demand for gluconate series products and gluconolactone at home and abroad has reached 700,000-800,000 tons, and the output has continued to expand. [0003] The production methods of calcium gluconate include electrolytic oxidation, bromination, metal-catalyzed synthesis, and fermentation. In industrialized large-scale production, metal c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08C12P7/58
CPCY02P20/50
Inventor 谭天伟陈文葛春玲陈必强
Owner BEIJING UNIV OF CHEM TECH
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