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Method for co-immobilizing anaerobic ammoxidation bacteria-short-cut nitrobacteria and application thereof

An anammox bacteria, anammox technology, applied in chemical instruments and methods, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc., can solve the problem of high Anammox cell density, The accumulation rate of nitrous is difficult to maintain stable and cannot be maintained, so as to achieve the effect of weakening the sludge loss rate, high mechanical strength and high water content

Inactive Publication Date: 2016-08-17
BEIJING UNIV OF TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Because the way to obtain nitrite by anaerobic ammonium oxidation is not easy to realize in low-temperature, low-carbon-to-nitrogen ratio urban domestic sewage, and the accumulation rate of nitrite is difficult to maintain stable, and a high Anammox cell density cannot be maintained in the reactor

Method used

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  • Method for co-immobilizing anaerobic ammoxidation bacteria-short-cut nitrobacteria and application thereof
  • Method for co-immobilizing anaerobic ammoxidation bacteria-short-cut nitrobacteria and application thereof

Examples

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Effect test

Embodiment 1

[0028] Example 1: Preparation of Anammox Bacteria-Short-range Nitrifying Bacteria Gel Beads

[0029]The anammox sludge with a sludge concentration of 8000mg / L was washed 3 times with a phosphate buffer solution with a pH of 7-7.5 and then used for later use. The short-course nitrification sludge with a nitrite accumulation rate > 90% was washed with deionized water for 3 times. Standby after repeated times; choose wooden powdery activated carbon, first wash to remove impurities and dust, and then soak for 24 hours with 10% hydrochloric acid, 2% sodium hydroxide, and normal saline with a concentration of 10% by mass. According to the concentration of 15g / L, 2g / L, 2g / L, prepare 500ml polyvinyl alcohol (PVA)-sodium alginate (SA)-activated carbon mixed solution ①, heat at 120°C for 20min to accelerate the dissolution; according to 50g / L and 2g / L mass-volume percent concentration preparation 1000ml sodium nitrate (NaNO 3 ) and calcium chloride (CaCl 2 ) mixed fixative ②. Embeddi...

Embodiment 2

[0030] Example 2: Co-immobilization process

[0031] The system is composed of urban sewage raw water tanks, biological carbon removal reactors, intermediate water tanks and full autotrophic denitrification reactors in series; urban sewage raw water tanks are connected to the inlet pipes of biological carbon removal reactors through the first water inlet pump; biological carbon removal The reactor is equipped with a first air compressor, a first aeration head, a first agitator, a first vent valve and an outlet valve; the outlet valve of the biological carbon removal reactor is connected to the intermediate water tank through the outlet pipe; the intermediate water tank is connected through the second inlet The water pump is connected to the water inlet valve of the fully autotrophic denitrification reactor; the fully autotrophic denitrification reactor is equipped with a second air compressor, a second aeration head, a second vent valve, a second outlet valve, a second agitator...

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Abstract

A method for co-immobilizing anammox bacteria-short-range nitrifying bacteria and its application belong to the technical field of sewage treatment, using polyvinyl alcohol, sodium alginate and activated carbon as embedding agents to anaerobic ammonium oxidizing bacteria and short-range nitrifying bacteria Layered embedding and fixation, firstly mix equal volumes of anammox sludge and embedding agent uniformly, get anaerobic ammonium oxidation gel pellets after fixation, and then put them into the mixed solution of short-range nitrifying bacteria and embedding agent In the process, anammox-short-range nitrifying bacteria gel pellets were obtained after fixation, and were directly added to the autotrophic denitrification reactor after activation. The present invention uses immobilized microbial technology to co-immobilize anammox bacteria and short-range nitrification bacteria on the same carrier, and stably enrich high-activity anammox and short-range nitrification sludge, so that the biological efficacy of the two bacteria can be coordinated. , to realize the coordinated operation of short-range nitrification reaction and anammox reaction.

Description

technical field [0001] The invention relates to a method for jointly immobilizing anammox bacteria and short-range nitrifying bacteria, which is applied in a process, and belongs to the technical field of sewage treatment. The specific content is to immobilize the anammox bacteria and short-range nitrifying bacteria together in the gel pellets by using immobilized microorganism technology, and inoculate the gel pellets containing the anammox bacteria and short-range nitrifying bacteria in the autotrophic Nitrogen removal reactor, the urban domestic sewage enters the autotrophic denitrification reactor after the organic matter is removed by the biological carbon removal reactor, part of the ammonia nitrogen in the domestic sewage is converted into nitrite by short-range nitrifying bacteria, and the nitrite is then anaerobic ammonium oxidation The process reacts with the remaining ammonia nitrogen and converts it into nitrogen, realizing the removal of nitrogen in urban domestic...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/08C12N11/04C02F9/14C02F3/30C02F3/34
CPCC12N11/14C02F3/307C02F3/34C02F9/00C12N11/04C12N11/08C12N11/10
Inventor 王淑莹韩金浩贾方旭张向晖彭永臻王众
Owner BEIJING UNIV OF TECH
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