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Method and apparatus for immobilizing cells to treat wastewater

A technology for treating waste water and solidified matter, applied in the fields of immobilized on or in biological cells, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of high organic solvent content, difficult selection of bacterial flora, high operating costs, etc. , to achieve the effects of improving tolerance and degradation ability, increasing oxidation reaction rate, and simple device structure

Inactive Publication Date: 2008-04-30
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the wastewater discharged from the production of phenolic resins and plasticizers has complex components, high content of COD and phenol, and high content of organic solvents
This kind of wastewater cannot (easily) survive in it, so most of them use organic solvent extraction to make the phenol content below 200mg / L, and then use biological methods to treat it, but the phenol recovered by the extraction method cannot be directly reused due to the large amount of impurities in it. , the operating cost is high, and the waste water is returned to the secondary pollution problem
Some people use immobilized large edible fungus mycelium to treat phenol-containing wastewater, but the phenol content in the treated wastewater can only be <600mg / L. When the phenol content in water is higher than 600mg / L, the phenol removal effect is very poor. There is no continuous use time provided
In short, some people use a variety of microorganisms to treat wastewater synergistically, but the selection of bacterial flora is a difficult problem, and it is also a difficult problem to use immobilized cell technology that is suitable for multiple strains at the same time, so this kind of wastewater has always been difficult to treat

Method used

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  • Method and apparatus for immobilizing cells to treat wastewater

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The method of increasing the content of plasticizer production wastewater in the culture medium is used for domestication, and the tolerance and degradation ability of the white rot fungus Versicolor versicolor (Polyporus versicolor NG04010) are gradually improved. The white-rot fungus Versicolor Versicolor Versicolor NG04010 was cultured for 7 days to collect mycelia. The culture method was a conventional method, and the culture medium was mashed potatoes mixed with wheat bran.

[0037] Pseudomonas fluorescens ATCC11172 (Pseudomonas fluorescens ATCC11172) was cultivated for 3 days, and the sludge was collected by centrifugation. The culture method was a conventional method, and the culture medium was malic acid 10g / L, (NH 4 ) 2 SO 4 2g / L and yeast extract 1g / L.

[0038] Mix the above-mentioned mycelia and fungus slime collected in a ratio of 5:1 to obtain a mixture, and then stir the mixture into about 50-100 times (weight ratio to the mixture) of the discarded rhiz...

Embodiment 2

[0041] The method of increasing the content of landfill leachate in the culture medium is used for domestication, and the tolerance and degradation ability of the white rot fungus Pleurotus pulmonarius NG 0412 are gradually improved. The domesticated white rot fungus Pleurotus pulmonarius NG 0412 was cultured in a solid state for 7 days to collect mycelium. The culture method was a conventional method, and the culture medium was mashed potatoes mixed with wheat bran.

[0042] Nitrosomonas (Nitrosomonas europaea IFO14298) was cultivated for 7 days, and the sludge was collected by centrifugation. The cultivation method was a conventional method, and the culture medium contained 0.3g of NaCl and MgSO in every liter of water. 4 ·7H 2 O 0.14g, FeSO 4 ·7H 2 O 0.03g, (NH 4 ) 2 SO 4 0.6g, KH 2 PO 4 2g.

[0043] Mix the above-mentioned mycelia and fungus slime collected in a ratio of 3:1 to obtain a mixture, and then mix the mixture into about 50-100 times (weight ratio to the ...

Embodiment 3

[0046] The method of increasing the content of phenolic resin production wastewater in the culture medium is used for domestication, and the tolerance and degradation ability of the white rot fungus Phanerochaete Chrysosporium (Phanerochaete Chrysosporium ME-446) are gradually improved. The white rot fungus (P.Chrysosporium ME-446) was cultured in a solid state for 7 days to collect mycelium, the culture method was a conventional method, and the culture medium was mashed potatoes mixed with wheat bran.

[0047] Rhodococcus erythropolis (Rhodococcus erythropolis NG0402) was cultivated for 3 days, and the sludge was collected by centrifugation. The cultivation method was a conventional method, and the culture medium was glucose 20g L -1 , protein building 20g·L -1 , yeast extract 10g·L -1 , K 2 HPO 4 2g·L -1 , MgSO 4 ·7H 2 O 0.5g·L -1 , pH 7.0-7.2.

[0048] Mix the above-mentioned mycelia and fungus slime collected in a ratio of 7:1 to obtain a mixture, and then mix the ...

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Abstract

The invention discloses a method and apparatus for immobilizing cells to treat wastewater wherein abandoned mycelium is used as a carrying agent for co-immobilization of white-rot fungi and bacteria, the enzyme system in the bacterium group is employed for coupled catalytic degradation of heterocyclic compounds and phenol in the waste water at the presence of oxygen supply. The device includes a reactor with moving sieving board and an oxygen supplying device.

Description

technical field [0001] The invention belongs to the field of wastewater treatment, and relates to a method for treating wastewater containing high-concentration phenol and organic solvents by using immobilized cells, and also relates to a device used in the method. Background technique [0002] At present, the wastewater discharged from the production of phenolic resin and plasticizer has complex components, high content of COD and phenol, and high content of organic solvents. This kind of wastewater cannot (easily) survive in it, so most of them use organic solvent extraction to make the phenol content below 200mg / L, and then use biological methods to treat it, but the phenol recovered by the extraction method cannot be directly reused due to the large amount of impurities in it. , the operating cost is high, and the waste water is returned to the secondary pollution problem. Some people use immobilized large edible fungus mycelium to treat phenol-containing wastewater, bu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N11/16
Inventor 范伟平欧阳平凯吴晟旻沈珈琦
Owner NANJING TECH UNIV
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