32results about How to "Immobilization method is simple" patented technology

A process for producing biological diesel oil by fixed enzyme method is carried out by taking sodium alginate, kaoline, glutaraldehyde, gelatin and lactose as co-fixer, fixing the lipase, piling the spherical fixed enzyme in filled bed columnar reactor naturally, alcoholyzing animal and plant oil fatty with biological diesel oil as solvent and producing biological diesel oil. It is cheap, has less consumption and by product and better quality. It can be used for continuous production.

Technology for fixing fungi and bacterium with vermiculite as carrier is carried out by modifying while moistening for vermiculite as carrier material, steam sterilizing at high-temperature and high-pressure, inoculating mixed bacterium, and multiplication culturing to obtain the final product. It's simple, porous and efficient, has better growth environment, degradation and resistance to poisonous, larger specific area and competition ability.

The invention discloses an ampere type biosensor electrode and a method for making the same. Dimethylformamide is taken as solvent, acrylonitrile copolymer and an electric mediator are taken as raw materials, an electrochemical electrode is taken as an accepting electrode, a composite fiber membrane of the acrylonitrile copolymer and the electric mediator is prepared on the surface of the electrode through electrostatic spinning, then a covalent method is used to fix xidoreductase on the surface of fibers to form an activated layer of the fibers on the surface of the electrode. The activated layer of the fibers has large specific surface area, high porosity rate and a through-hole structure; at the same time, the electric mediator is provided, so the electrode has higher response strength, linear detection range and stability. The mediator electrochemical enzyme electrode has the characteristics of simple preparation process, repeated use, large surface area of an effective enzyme membrane, strong anti-jamming ability and so on, and is suitable for batch production.

The invention relates to a cell immobilization technology, in particular to an immobilization bacteria production method used for repairing soil contaminated by polycyclic aromatic hydrocarbon. The carrier material is 0.25 to 1.00mm of red brick powder, fly-ash, vermiculite, peat soil, diatomite or coke powder, which are sterilized and added with bacteria liquid to do multiplication culture, so as to make the required immobilized particles. The immobilized biological product made according to the prescription of the invention has good repairing effect on the soil contaminated by the polycyclic aromatic hydrocarbon and is a reliable, novel and environment-friendly biological product.

The invention discloses an immobilized recombinant penicillin G acylase and an application thereof to preparation of (S)-2-aryl-amino acid and cephalosporin antibiotics. The immobilized recombinant penicillin G acylase disclosed by the invention is capable of converting an S-type substrate into an S-type product within shorter time, high in substrate tolerance and strict in S selectivity for the substrate and structural analogues thereof; due to the addition of cobalt ions as well as a protective agent with phenylacetic acid and glycerin as enzyme active centers, the immobilized recombinant penicillin G acylase disclosed by the invention is prevented from being successfully subjected to multipoint covalent immobilization on the surface of an epoxy resin carrier under the condition that enzyme molecules are seriously inactivated in an immobilization process, and the immobilization method is simple and feasible, cheap in raw materials and suitable for large-scale operation; the immobilized recombinant penicillin G acylase can be used for synthesizing cephalosporin antibiotics and splitting the prepared (S)-2-aryl-amino acid, and can be continuously used for more than 50 batches before the activity of the immobilized enzyme is not remarkably reduced.

Production of fixed bacterium as peat as carrier is carried out by alkalizing while moistening for peat, taking it as carrier material, steam sterilizing at high-temperature and high-pressure, inducing into bacterial suspension, multiplication culturing to obtain the final product. It's simple and efficient, has better growth environment, degradation and binding functions and excellent affinity ability.

The invention relates to the cell immobilization technology, specifically speaking, it's about the preparing method of a solidified granule which can be used in the repair of soil which is polluted by polycyclic aromatic hydrocarbon. The main carrier is corncob. Mix the fresh, dry and pulverized corncob (the diameter is about 1.5-2cm) with cotton kernel pastry, wheat bran, bean cake powder, rice bran and / or wheat bran, of which the corncob accounts for 70-85%, the accessories account for 15-30%; regulate the water content to 40-55wt% and pH value to 6.3-6.5; after autoclaving, inoculate the foundation stock full of fungal spore and enrichment cultivate to get the solidified granule. The advantage of the invention includes: low cost, high solidifying efficiency, simple operating method and suitable to repair the soil in large scale.

The invention provides a production method of inosinic acid. The method comprises the step of adopting adenosine deaminase fixed in anion resin to catalyze the generation of the inosinic acid from adenylic acid. According to the method provided by the invention, the adenylic acid is taken as a substrate, the supply of raw materials is sufficient, the requirements for equipment are low, the separation and extraction are convenient, the semi-continuous production of the inosinic acid can be realized, the substrate is convenient and easy to obtain, the transformation is thorough, and the cost is relatively low.

The present invention provides a gene modification method to realize its soluble and active expression: take the lipase gene with polyamine tags at the N-terminus and C-terminus as the target gene, and add coding A nucleotide sequence of m histidines, m arginines or m lysines, m=2-10, and at the same time add n histidines and n sperms to the C-terminus of the lipase parent gene sequence amino acid or n lysine nucleotide sequence, n=2-10, and primers are designed for the target gene; the N-terminal and C-terminal lipase genes with polyamine tags are obtained by PCR. After the gene is expressed, a soluble lipase with a polyamine tag is produced, which can induce the formation and immobilization of biomimetic nano-silicon. The invention can obtain the immobilized enzyme with high activity and immobilization efficiency under mild conditions and in a short time; the operation is simple, the immobilization conditions are easy to control, and the cost is low.

The invention provides a method for utilizing a lactobacillus fermented agricultural and sideline product to prepare gamma-aminobutyric acid, and belongs to the technical field of biological processing of agricultural and sideline products. The method comprises the steps that S1, lactobacillus bacterial suspension liquid is prepared; S2, lactobacillus is fixed; and S3, the fixed lactobacillus obtained in the step S2 is inoculated to a liquid culture medium containing the agricultural and sideline product and sufficiently stirred, and the liquid ferments. Lactobacillus is fixed by utilizing sericin, cellulose and oxidized dextran containing polyaldehyde, and the lactobacillus fermented agricultural and sideline product has the characteristics that the biocompatibility is high, the product is safe and non-toxic, the stability is good, and multiplication of lactobacillus is promoted. The waste agriculture and sideline product is adopted to be a fermentation culture substrate, resource recycling is achieved, and the obtained fermented liquid contains the nutrient matter in the agricultural and sideline product and a great amount of gamma-aminobutyric acid, and has the very high nutrient value.

The invention discloses an L-lactic acid fermentation method by using polyvinyl alcohol to solidify rhizopus oryzae. The method comprises the following steps: heating polyvinyl alcohol and sodium algiThe invention discloses an L-lactic acid fermentation method by using polyvinyl alcohol to solidify rhizopus oryzae. The method comprises the following steps: heating polyvinyl alcohol and sodium alginate in a boiling water bath to be jointly dissolved; mixing with a rhizopus oryzae spore suspension; using an injector to drip the mixed solution to a saturated boric acid solution containing calciumnate in a boiling water bath to be jointly dissolved; mixing with a rhizopus oryzae spore suspension; using an injector to drip the mixed solution to a saturated boric acid solution containing calcium chloride while using a magnetic stirrer to continuously stir so that particles are spherical and good immobilized particles can be hardened at a low temperature; and then, treating by a monobasic sodchloride while using a magnetic stirrer to continuously stir so that particles are spherical and good immobilized particles can be hardened at a low temperature; and then, treating by a monobasic sodium phosphate solution and washing with distilled water after solidified cells are propagated and grow so as to be used for the batch fermentation and the repetitive batch fermentation of the L-lacticium phosphate solution and washing with distilled water after solidified cells are propagated and grow so as to be used for the batch fermentation and the repetitive batch fermentation of the L-lactic acid. The improved L-lactic acid fermentation method by using the polyvinyl alcohol to solidify the rhizopus oryzae overcomes the defects of spherical carriers and poor permeability of the prior polyacid. The improved L-lactic acid fermentation method by using the polyvinyl alcohol to solidify the rhizopus oryzae overcomes the defects of spherical carriers and poor permeability of the prior polyvinyl alcohol and has the advantages of great mechanism strength, large acid production amount, high acid production speed and good repetitive use effect.vinyl alcohol and has the advantages of great mechanism strength, large acid production amount, high acid production speed and good repetitive use effect.