A method for preparing xanthus sorbifolium polypeptide based on papaya seed gum immobilized enzyme
A papaya seed gum, immobilized enzyme technology, applied in the directions of immobilization on/in organic carriers, hydrolase, fermentation, etc., can solve the problems of high immobilization cost, few immobilized enzymes, poor stability, etc., and achieves immobilization. Simple chemical method, good stability and good mechanical properties
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Embodiment 1
[0016] 1. Dissolve 1g of papain, 3g of papaya seed gum and 3.8g of chromium acetate in phosphate buffer solution of 100g of pH=6.5 respectively to prepare papain solution with a mass concentration of 1% and papaya seed gum with a mass concentration of 3%. solution, a mass concentration of 3.8% chromium acetate solution; after mixing 20mL mass concentration of 3% papaya seed gum solution with 10mL mass concentration of 1% papain solution, add 60mL mass concentration of 3.8% chromium acetate solution , standing at 40° C. for 6 hours, and then centrifuging at 5000 rpm, and freeze-drying the precipitate to obtain papaya seed gum-immobilized enzyme.
[0017] 2. After adjusting the pH value to 6.5 with phosphate in the 2% aqueous solution of radix sorbifolia protein, add papaya seed gum to immobilize the enzyme according to the ratio of 3300U papain / g sorbifolia protein, and enzyme at 55°C Decompose for 60 minutes, after enzymolysis finishes, carry out centrifugation at a centrifuga...
Embodiment 2
[0019] 1. Dissolve 1.5g of protease (trypsin and neutral protease in an activity ratio of 1:1 mixture), 2.5g of papaya seed gum, and 3g of chromium propionate in 100g of phosphate buffer at pH=8.0, and prepare the mass concentration 1.5% protease solution, 2.5% papaya seed gum solution, 3% chromium propionate solution; 20mL 2.5% papaya seed gum solution and 1.5% protease After the solution was mixed evenly, 40 mL of chromium propionate solution with a mass concentration of 3% was added, and it was left to stand at 50° C. for 3 hours, then centrifuged at a speed of 5000 rpm, and the precipitate was freeze-dried to obtain papaya seed gum-immobilized enzyme.
[0020] 2. Adjust the pH value of the 4.5% sorbifolia protein aqueous solution to 8.0 with phosphate, add papaya seed gum to immobilize the enzyme according to the ratio of 3300U protease / g sorbifolia protein, and enzymatically hydrolyze at 50°C 180 minutes, after enzymatic hydrolysis, centrifugal separation was performed at...
Embodiment 3
[0022] 1, 2.5g alkaline protease, 4g papaya seed gum, 2.5g chromium acetate are dissolved in the phosphate buffer solution of 100g pH=9.0 respectively, are mixed with the alkaline protease solution that mass concentration is 2.5%, the mass concentration is 4% Papaya seed gum solution, a mass concentration of 2.5% chromium acetate solution; after mixing 20mL of a mass concentration of 4% papaya seed gum solution with 10mL of a mass concentration of 2.5% alkaline protease solution, adding 24mL of a mass concentration of 2.5% The chromium acetate solution was allowed to stand at 45° C. for 2.5 hours, then centrifuged at a speed of 5000 rpm, and the precipitate was freeze-dried to obtain papaya seed gum-immobilized enzyme.
[0023] 2. After adjusting the pH value to 9.0 with phosphate in the aqueous solution of radix sorbifolia protein with a mass concentration of 3%, add papaya seed gum to immobilize the enzyme according to the ratio of 4000U alkaline protease / g radix sorbifolia p...
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