Amino-acid oxidase supported catalyst used for oxidizing amino acid to prepare ketonic acid
A supported catalyst and amino acid technology, applied in the direction of organic compound/hydride/coordination complex catalysts, physical/chemical process catalysts, chemical instruments and methods, etc., can solve the problem of low yield of ketoacids, regeneration of amino acid oxidase catalysis Reduce activity and other problems, achieve the effect of enhanced surface activity, excellent surface adsorption activity, and enhanced lipophilicity
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Embodiment 1
[0030] (1) Preparation of co-fixative: add 0.5 g of microcrystalline cellulose and 0.05 g of ultrafine magnesium oxide to 1.5 g of poloxamer, and heat up to 115-120 ° C at a heating rate of 5 ° C / min and mix for 15 min , then add 0.3g cationic polyacrylamide and 0.05g sodium alginate, continue to mix at 115-120°C for 15 minutes, transfer the resulting mixture to an environment of 0-5°C and keep it sealed for 1h, and heat up again at a rate of 5°C / min Speed up to 115-120°C and keep mixing for 15 minutes, then add 10g of 60-65°C water, mix well, keep it at 60-65°C for 30 minutes, filter, and send the obtained filter residue into a spray dryer, and dry the obtained particles through ultra- Micro pulverizer into fine powder, that is, the co-fixative agent;
[0031] (2) Preparation of composite carrier: add 2.5g of ceramic micropowder, 0.3g of polyaspartic acid and 0.05g of sodium citrate to 10g of water, heat up to a reflux state at a heating rate of 5°C / min and mix for 15min,...
Embodiment 2
[0037](1) Preparation of co-fixative: add 0.5 g of microcrystalline cellulose and 0.05 g of ultrafine magnesium oxide to 2 g of poloxamer, and heat up to 115-120 ° C at a heating rate of 5 ° C / min and mix for 15 min. Then add 0.5g cationic polyacrylamide and 0.05g sodium alginate, continue to heat and mix at 115-120°C for 15min, transfer the resulting mixture to a sealed environment at 0-5°C for 1h, and heat up again at a rate of 5°C / min Heat up to 115-120°C and keep mixing for 15 minutes, then add 10g of 60-65°C water, mix well and keep it at 60-65°C for 30 minutes, filter, and send the obtained filter residue into a spray dryer, and dry the obtained particles through ultrafine Grinding machine into fine powder, that is, the co-fixative agent;
[0038] (2) Preparation of composite carrier: add 2.5g of ceramic micropowder, 0.3g of polyaspartic acid and 0.05g of sodium citrate to 10g of water, heat up to a reflux state at a heating rate of 5°C / min and mix for 15min, filter, a...
Embodiment 3
[0044] (1) Preparation of co-fixative: add 0.5 g of microcrystalline cellulose and 0.05 g of ultrafine magnesium oxide to 2 g of poloxamer, and heat up to 115-120 ° C at a heating rate of 5 ° C / min and mix for 15 min. Then add 0.5g cationic polyacrylamide and 0.05g sodium alginate, continue to heat and mix at 115-120°C for 15min, transfer the resulting mixture to a sealed environment at 0-5°C for 1h, and heat up again at a rate of 5°C / min Heat up to 115-120°C and keep mixing for 15 minutes, then add 10g of 60-65°C water, mix well and keep it at 60-65°C for 30 minutes, filter, and send the obtained filter residue into a spray dryer, and dry the obtained particles through ultrafine Grinding machine into fine powder, that is, the co-fixative agent;
[0045] (2) Preparation of composite carrier: add 2.5g of ceramic micropowder, 0.3g of polyaspartic acid and 0.05g of sodium citrate to 10g of water, heat up to a reflux state at a heating rate of 5°C / min and mix for 15min, filter, ...
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