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Method for extracting myricetin through microbial fermentation

A microbial fermentation and extract technology, applied in the directions of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of reducing the purity of myricetin, difficult to remove residual substances, low safety, etc., so as to avoid the degradation of fermentation quality. , Improve the extraction yield and purity, and improve the effect of yield and purity

Active Publication Date: 2019-12-31
宁波德康生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these strong acids are industrial raw materials that are easy to pollute the environment, and the safety is not high, and the residual substances after strong acid hydrolysis of myricetin are difficult to remove, reducing the purity of myricetin

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] In the present embodiment 1, the extraction method of myricetin comprises the following steps:

[0047] 1) Construction of immobilized cells: the Aspergillus niger strain was cultured on a solid slant medium for 7 to produce a large number of spores, and the spores were placed in physiological saline to make a content of 3×10 8 Aspergillus niger spore suspension in CFU / ml; Lactobacillus in the logarithmic growth phase was resuspended in normal saline to obtain a lactobacillus content of 2 × 10 10 CFU / ml of Lactobacillus suspension. Mix the Aspergillus niger spore suspension and the Lactobacillus cell suspension at a volume ratio of 1:1, then add it to the sodium alginate solution so that the final concentration of sodium alginate is 3%, stir the mixture evenly, and drop it in with a syringe to 1% CaCl 2 In the solution, co-immobilized gel beads with a diameter ranging from 2.5 to 3 mm were formed and placed in a refrigerator at 4° C. for 10 h to solidify.

[0048] 2)...

Embodiment 2

[0054] In the present embodiment 1, the extraction method of myricetin comprises the following steps:

[0055] 1) Construction of immobilized cells: the Aspergillus niger strain was cultured on a solid slant medium for 6 to produce a large number of spores, and the spores were placed in physiological saline to make a content of 7×10 8 CFU / ml of Aspergillus niger spore suspension; Lactobacillus in the logarithmic growth phase was resuspended in normal saline to obtain a lactobacillus content of 6 × 10 10 CFU / ml of Lactobacillus suspension. Mix the Aspergillus niger spore suspension and the Lactobacillus cell suspension at a volume ratio of 1:1, then add it to the sodium alginate solution so that the final concentration of sodium alginate is 4%, stir the mixture evenly, and drop it in with a syringe to 1.5% CaCl 2 In the solution, co-immobilized gel beads with a diameter ranging from 2.5 to 3 mm were formed and placed in a refrigerator at 4° C. for 11 hours to solidify.

[00...

Embodiment 3

[0062] The difference between embodiment 3 and embodiment 1 is only that step 3) of embodiment 3) the fermentation temperature is 40°C, and the others are the same as in embodiment 1.

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Abstract

The invention belongs to the technical field of separation of natural products, and relates to a method for extracting myricetin through microbial fermentation. The method comprises the following steps of (1) preparing co-immobilization gel beads containing aspergillus niger spore suspension and lactobacilli suspension; (2) mashing myricetin extraction raw materials, adding ethanol, performing reflow extraction for 1-5 times, combining filtrate, performing concentration, and drying filter residues; (3) adding water to a fermenter, adding the co-immobilization gel beads obtained in the step (1), an extract obtained in the step (2) and 1 / 5-4 / 5 of dried filter residues, and performing fermentation and extraction at 40-50 DEG C for 140-170h; (4) after the fermentation is completed, filtratingfermentation liquid with a screen mesh, and removing the co-immobilization gel beads; (5) performing reflow extraction on the filtered fermentation liquid with ethanol for 1-5 times, combining filtrate, and performing concentration so as to obtain precipitate; and (6) repeatedly washing the precipitate with water for 4-6 times so as to obtain finished products. Through the adoption of the extraction method, the yield and the purity of the myricetin are greatly increased, and the method is environmentally-friendly and safe.

Description

technical field [0001] The invention belongs to the technical field of natural product separation, and relates to a method for extracting myricetin by microbial fermentation. Background technique [0002] Myricetin [3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-1-benzofuran-4-one, Myricetin] also known as myricetin, myricetin , myricetin, myricetin, is a polyhydroxy flavonol compound, yellow needle crystal, melting point is 324.0 ~ 325.5 ℃, easily soluble in methanol, ethanol, acetone, ethyl acetate, slightly soluble in water, hardly soluble in Chloroform, petroleum ether. Modern pharmacological studies have shown that myricetin has various pharmacological activities such as anti-inflammatory and analgesic, anti-tumor, hypoglycemic, liver protection and anti-oxidation, especially in the prevention and treatment of cardiovascular diseases. In view of its excellent antioxidant function and cholesterol-lowering effects, extracting myricetin from plants has become a research ...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12R1/225C12R1/685
CPCC12P17/06
Inventor 王明谦纪东艺姚恩琪
Owner 宁波德康生物制品有限公司
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