44 results about "Bovine hemoglobin" patented technology

The invention provides a preparation method for a thermo-sensitive type molecularly imprinted polymer with a metal frame. The method is used for enriching or sensing bovine hemoglobin and comprises the following steps: preparing a metal frame compound with upconversion fluorescence; introducing a thermo-sensitive monomer. According to the preparation method for the thermo-sensitive type molecularly imprinted polymer of the metal frame, UCNPs with strong fluorescence and MOF with larger specific surface area are introduced into a molecular imprinting technology, and a prepared composite material can be used for enriching or sensing the bovine hemoglobin. Meanwhile, the thermo-sensitive monomer is introduced, so that the composite material has a temperature-based reversible adsorption / release function and has a broad application prospect.

The invention discloses a middle / low concentration positive quality control liquid for urine analysis. The positive quality control liquid comprises a buffer solution with a pH value of 4.5 to 8.5 and a concentration of 20 to 200 mmol / L, a surfactant with the concentration of 20 to 200 mmol / L, a carbohydrate protectant with the concentration of 20 to 200 mmol / L, a protein protectant with the concentration of 20 to 200 mmol / L, anhydrous glucose with the concentration of 300 to 990 mg / L, bovine serum albumin with the concentration of 100 to 1000 mg / L, bovine hemoglobin with the concentration of0.6 to 5 mg / L, esterase with the concentration of 2 to 20 mg / L, acetone or lithium acetoacetate or sodium ethyl acetoacetate or ethyl acetoacetate with the concentration of 50 to 900 mg / L, and a direct bilirubin or naphthylamine salt with the concentration of 3 to 40 mg / L. Quality control objects of corresponding projects can be added as needed. Compared with the prior art, the quality control liquid has the advantages of good stability, high sensitivity, less toxicity of each component, wide source and low production cost.

The invention belongs to the fields of material science and engineering and biological separation engineering, and particularly relates to a quantum dot-based protein imprinted polymer microsphere as well as preparation and application thereof. The CdTe quantum dot is directly used as a functional monomer through taking mercaptoacetic acid (TGA) and glutathione (GSH) together as a stabilizer, protein is a template molecule, dopamine is a cross-linking agent, and the imprinted polymer microsphere is obtained through the auto polymerization of the dopamine. The quantum dot-based protein imprinted polymer microsphere as well as the preparation and the application thereof provided by the invention have the advantages that fluorescence signals of the quantum dots are utilized for detection, and the functionalized quantum dots are directly taken as functional monomers, so that the quantum dots are contained in imprinted holes. Therefore, the detection sensitivity can be improved. Meanwhile, an imprinted polymer layer is obtained through the auto polymerization of the dopamine, and the imprinting process is further simplified. The quantum dot-based protein imprinted polymer microsphere is used for analysis and detection on phycocyanin in a sea water and lake water sample and bovine hemoglobin in a bovine urine sample, the adding standard recovery rate can reach 90 percent or higher, and the reuse performance is good.

The invention discloses a method for preparing haemoglobin polypeptide with bovine hemoglobin as the raw material. The method is characterized by mainly comprising the following steps of 1, raw material preparation, wherein 600 g of hemoglobin crude products are selected, and water is added to the crude products to dilute the crude products till the concentration is 5%-6%; 2, primary enzymolysis, wherein 100-150 g of flavourzyme is added, pH is 6.5-7.5, and hydrolysis is conducted for 4.5-5.5 hours at the temperature of 50-55 DEG C; 3, cooling, wherein after the primary enzymolysis, the temperature is lowered to be 43-46 DEG C, and the pH is adjusted to 8.5-9.5; 4, secondary enzymolysis, wherein 60-70 g of alkaline protease is added, and enzymolysis is conducted for 4.5-5.5 hours; 5, high-temperature enzyme deactivation, wherein water bath enzyme deactivation is conducted for 10-20 minutes; 6, centrifugation, wherein sediment is conducted, liquid supernatant is obtained, cooling and drying are conducted, and the haemoglobin polypeptide is obtained. Compared with the prior art, the method has the advantages that the production process is simple, and a prepared polypeptide product is rich in heme, low in bitterness value and the like.

The invention discloses molecular imprinted magnetic nanoparticle capable of specifically adsorbing bovine hemoglobin, which belong to the technical field of biochemical separation. The molecular imprinted functionalized magnetic nanoparticles having the capability of specifically adsorbing a target protein can be prepared, the adsorption and separation conditions for bovine hemoglobin are mild bythe nanoparticles, the reaction is simple and rapid, and the specific adsorption of the target protein-bovine hemoglobin can be achieved in a protein mixed solution, which lays a good foundation forthe separation and purification of proteins.

The invention specifically relates to a fluorescence carbon spot as well as a preparation method and application thereof and belongs to the field of novel nano material preparation. The fluorescence carbon spot preparation method disclosed by the invention comprises the specific steps: cleaning leaves, putting in an oven to be dried and smashing and screening to obtain leaf powder; adding deionized water into the leaf powder, then adding a polyethyleneimine solution into a magnetic stirring process and continuing magnetically stirring under the room temperature; centrifuging, collecting supernatant and filtering and dialyzing the supernatant to obtain fluorescence carbon spot dispersion liquid. The preparation method disclosed by the invention has simpleness, convenience and safety; meanwhile, use of expensive instruments is reduced; the raw materials are low in cost, environmentally friendly and renewable. The fluorescence carbon spot disclosed by the invention has the advantages of uniform particle size and evenness in dispersion; the fluorescence carbon spot has high detection flexibility to bovine hemoglobin, a linear range is 0 to 54 mu g / L, a complex modified process is avoided, a detection method is simple, and good optical detection capacity is achieved. The fluorescence carbon spot disclosed by the invention has a wide application prospect in detecting bovine hemoglobin.

The invention belongs to the field of analytical chemistry and quick detection, and particularly relates to a red-green-blue tri-fluorescent emission molecularly imprinted sensor based on a post-imprinting mixing method and a preparation method and application thereof in accurately and visually detecting hemoglobin. The red-green-blue tri-fluorescent emission molecularly imprinted sensor is obtained by mixing red fluorescent bovine hemoglobin imprinted microspheres, green fluorescent bovine hemoglobin imprinted microspheres and blue fluorescent bovine hemoglobin imprinted microspheres throughthe post-imprinting mixing method. The sensor prepared by the method can detect bovine hemoglobin in a high-sensitivity, high-selectivity and self-correcting manner, and the sensor is wider and richerthan conventional dual-fluorescent emission molecularly imprinted sensors in fluorescent color changing range and can visually detect a target more accurately.

The invention discloses a tolyltriazole-containing polyethylene glycol protein modifier, a preparation method thereof and the application thereof, wherein the tolyltriazole-containing polyethylene glycol protein modifier has the following chemical general formula, wherein A is linear-chain alkyl group of which the carbon atom number is 0-20. The preparation method of the modifier comprises the following step of carrying out copper-catalyzed click chemistry on azide group-containing methoxy polyethyleneglycol and terminal acetylene link and succinimide-containing alkane derivative. The tolyltriazole-containing polyethylene glycol protein modifier provided by the invention can be used for modifying the hemoglobin, particularly used for modifying the bovine hemoglobin and the hemoglobin from human blood for human body. According to the method of the copper-catalyzed click chemistry, various functional groups can be effectively modified, and the invention is simple in technology, low in cost, and convenient for industrial production.

The invention relates to a method for preparing a bovine hemoglobin / titanium-based titanium dioxide hybrid material and belongs to the technical field of biological-inorganic hybridization. The method comprises the following steps of: preparing a titanium-based titanium dioxide nano array tubular film; and preparing a hemoglobin and titanium-based titanium dioxide nano array tube hybrid film. A highly ordered titanium dioxide nanotube array film is prepared by an anode oxidation method and the hemoglobin is fixed to the titanium-based titanium dioxide nanotube array film by using electrostatic self-assembly technology. The method has the advantages of simple operation and good enzyme activity keeping. The prepared hybrid film can be used in the fields of bio-sensing, biological electrochemical apparatuses and the like.

The invention relates to a novel magnetic bead capable of being used for separating, purifying and immobilizing histidine tag protein and bovine hemoglobin as well as a preparation method and application of the novel magnetic bead. The preparation method comprises the following steps: firstly, a nano ferroferric oxide magnetic core is prepared by utilizing a co-precipitation method; and then, thesurface of the magnetic core is directly modified with a plurality of different carboxylated silane coupling agents as ligands, so that the magnetic core can chelate metal ions. The magnetic bead prepared by the method is good in magnetism, stable in property and easy to disperse, and has the advantage that the synthesis method is simple and easy to control and implement. Target proteins such as His-tagged protein and bovine hemoglobin can be separated and purified with high selectivity and high efficiency through stable combination of strong coordination between metal ions on the surface of the magnetic bead and histidine residues on the surface of a recombinant protein.

A process for preparing the poly-haematoglobin with homogeneous molecular weight includes adsorbing haematoglobin by solid phase, adding dual-function reagent, polymerizing reaction, eluting and collecting the polymer by buffering liquid, and chromatography for separating and purifying the polyhaematoglobin. Its advantages are homogeneous molecular weight (about 135 thosands Dol), easy separation, and lower osmotic pressure.

The invention discloses an Au NPs coated WP5 / BiOBr composite material as well as a preparation method and application thereof. The Au NPs coated WP5 / BiOBr composite material is prepared by loading Au NPs coated WP5 in a bismuth oxybromide nanoflower lamella, the Au NPs coated WP5 / BiOBr composite material is modified on the surface of a glassy carbon electrode, and the Au NPs coated WP5 / BiOBr composite material is prepared by utilizing the local surface plasma effect of gold nanoparticles under visible light. The host-guest complexing effect of water-soluble pillararene and the photogenerated electron hole acceleration redox reaction of BiOBr under visible light illumination are cooperated to detect the dopamine in the solution. Bovine hemoglobin with different concentrations is sequentially dropwise added into the solution, an electric signal generated by dopamine can be weakened, so that indirect detection of the bovine hemoglobin is achieved, the detection range is 10<-11>-10<-1> mg / mL, and the detection limit is 4.2* 0<-12> mg / mL.

The embodiment of the invention discloses a surfactant-free urine analysis quality control liquid. The surfactant-free urine analysis quality control liquid comprises 5-50 mmol / L of a buffer solution,0.1-1 g / L of hydrolase, 50-500 mg / L of BSA, 0.1-1 g / L of bovine hemoglobin, 20-200 mg / L of sodium nitrite, 50-500 mg / L of calcium ions, 20-200 mg / L of a bilirubin substitute, 0.1-1 g / L of a urogen substitute, 0.1-1 g / L of ketone substitute, 50-500 mg / L of creatinine and 1-10g / L of sugar. The surfactant-free urine analysis quality control liquid disclosed by the embodiment of the invention does not need a surfactant or a protein protective agent, so that the real-time stability is improved, the toxicity is lower, the sensitivity is higher, and the detection result is more accurate; moreover, the production cost is lower, and the storage time is longer; and the surfactant-free urine analysis quality control liquid provided by the embodiment of the invention is a greener, more environment-friendly and more excellent urine analysis quality control liquid solution.

The invention provides a decitabine nano carrier and an application thereof in preparing a tumor fluorescence imaging agent. The decitabine nano carrier is prepared by a multiple emulsion method. Bovine hemoglobin, fluorescent dye Nile red, a polylactic acid-glycolic acid copolymer and the like are adopted for emulsification, then wrapping is performed by using chitosan loaded with decitabine andchitosan modified by nitrobenzooxadiazole (NBD), and sterilizing is performed to prepare freeze-dried powder, wherein the freeze-dried powder is dissolved in a water phase for oxygen loading before being used. The decitabine nano carrier is based on 769-P and OS-RC-2 kidney cancer cell lines, and a hypoxia cell model and a tumor-bearing mouse model are constructed. The results show that CNDHNNPs can improve the hypoxia microenvironment and reduce drug efflux, and decitabine can increase uptake of chemotherapeutic drugs and increase sensitization of chemotherapy. Peroxidation of lipid is increased by catalysis, and ferroptosis therapy is combined. The invention provides a novel scheme of hypoxia and hypermethylation combined ferroptosis of targeting tumor for renal cancer treatment, provides a drug delivery system, and realizes tumor fluorescence imaging. The decitabine nano carrier is reasonable in design and high in repeatability.

The invention discloses a dextran cross-linked hemoglobin-based oxygen carrier, a preparation method thereof and an application. The preparation method includes the steps: oxidizing hydroxy on dextran 40 into an aldehyde group by sodium periodate to obtain activated dextran; protecting sulfydryl on hemoglobin by 4-PDS; crosslinking the activated dextran with the bovine hemoglobin closed by the sulfydryl; finally, de-protecting the sulfydryl to obtain the dextran cross-linked hemoglobin-based oxygen carrier. P50 of the dextran cross-linked hemoglobin-based oxygen carrier (Dex-Hb) prepared by the method can be effectively increased, oxygen carrying-releasing capacity is improved, intramolecular crosslinking of hemoglobin tetramer is realized, so that tetramer stability of HBOCs is remarkably enhanced, and physiological functions of the HBOCs are further improved.

The invention relates to an anti-porcine hemoglobin hybridoma cell strain, and a monoclonal antibody and an application of the anti-porcine hemoglobin hybridoma cell strain, and belongs to the technical field of immunology. The anti-porcine hemoglobin hybridoma cell strain PHb-3B4 has a collection number of CCTCC NO (China Center for Type Culture Collection number): C201784. The anti-porcine hemoglobin monoclonal antibody is generated by the anti-porcine hemoglobin hybridoma cell strain; a subtype is IgG1 (immune globulin G1); the valence is 1:819200; an affinity constant is 2.8*10<8>L / mol; and cross reaction rates of the monoclonal antibody with bovine hemoglobin and human hemoglobin are less than 0.1%. The anti-porcine hemoglobin monoclonal antibody is used for detecting porcine hemoglobin, and has the advantages of high valence, good sensitivity and high specificity. The porcine hemoglobin monoclonal antibody can be used for developing an immunological diagnostic reagent related tothe porcine hemoglobin, such as a colloidal gold test strip, a fluorescence detection test strip and an ELISA (enzyme-linked immuno sorbent assay) reagent kit.

The invention provides a PolyHb-rPA complex, a preparation method and an application of the PolyHb-rPA complex. Reteplase (r-PA) is cross-linked with high-purity bovine hemoglobin (Hb) by glutaraldehyde to form the PolyHb-rPA complex, so that the stability and the half life of the r-PA in vivo after intravenous injection are effectively improved. The volume of the cross-linked complex PolyHb-rPA is hundreds of times smaller than that of red blood cells in the blood, the hemoglobin in the PolyHb-rPA can form capillaries to supply oxygen to embolized ischemic tissues, and the capillaries are narrow at an early stage. After the r-PA is cross-linked with the hemoglobin, the half life is prolonged, the activity is reduced, and the effect is more stable and durable, so that the risk of bleeding is reduced.

A protein modifier with long tail of monomethoxy polyethanol for modifying human or ox haematoglobin is prepared from the monomethoxy polyethanol with 750-10,000 of mass average molecular weight and L-glutamic acid through condensation reaction.

The invention discloses construction and application of a saccharomyces cerevisiae strain for efficiently synthesizing hemoglobin or myoglobin from different sources, and belongs to the technical field of genetic engineering. Proper expression vectors, chaperonin and promoters are selected, a host is modified, heterologous expression of the hemoglobin or myoglobin from different sources is achieved in saccharomyces cerevisiae, safety of the hemoglobin and myoglobin is guaranteed, efficient production of the hemoglobin or myoglobin is achieved, and the potential safety hazard that an expression host is not a food-grade host in the previous report is solved. At a shake flask level, the yield of soybean hemoglobin can at least reach 120 mg L<-1>, the yield of clover hemoglobin can at least reach 20 mg L<-1>, the yield of porcine myoglobin can at least reach 99 mg L<-1>, the yield of bovine myoglobin can at least reach 89 mg L<-1>, the yield of porcine hemoglobin can at least reach 20 mg L<-1>, and the yield of bovine hemoglobin can at least reach 23 mg L<-1>. The result lays a foundation for application of the hemoglobin / myoglobin in the field of processing of foods such as artificial meat.

The invention relates to a quantitative detection method of bovine genome DNA in a bovine hemoglobin product and application of the quantitative detection method, and particularly provides a fluorescent quantitative PCR detection method of the bovine genome DNA in the bovine hemoglobin product. The detection method comprises the steps of extracting DNA in the bovine hemoglobin product, and performing amplification detection on the extracted DNA by using the fluorescent quantitative PCR method. According to the method disclosed by the invention, the bovine DNA purified from artificially polymerized bovine hemoglobin is subjected to specific amplification and fluorescence detection, and rapid and accurate detection of bovine components in biochemical drug products is realized.

The invention discloses a tolyltriazole-containing polyethylene glycol protein modifier, a preparation method thereof and the application thereof, wherein the tolyltriazole-containing polyethylene glycol protein modifier has the following chemical general formula, wherein A is linear-chain alkyl group of which the carbon atom number is 0-20. The preparation method of the modifier comprises the following step of carrying out copper-catalyzed click chemistry on azide group-containing methoxy polyethyleneglycol and terminal acetylene link and succinimide-containing alkane derivative. The tolyltriazole-containing polyethylene glycol protein modifier provided by the invention can be used for modifying the hemoglobin, particularly used for modifying the bovine hemoglobin and the hemoglobin from human blood for human body. According to the method of the copper-catalyzed click chemistry, various functional groups can be effectively modified, and the invention is simple in technology, low in cost, and convenient for industrial production.

The invention relates to the technical field of biological catalytic synthesis and particularly discloses a method for synthesizing quinoxaline compounds by a double-protein catalytic cascade reaction. The method comprises the steps as follows: a beta-keto ester compound, substituted o-phenylenediamine and methylphenylsulfonyl azido are taken as reactants to be dissolved in a solvent, a catalyst and a surfactant are added, the mixture is stirred to react to produce a product, and the product is sequentially dried, concentrated and purified, wherein the solvent is water, and the catalyst is procine pancreaslipase (PPL) and hemoglobin from bovine blood (HbBv). The process is convenient, a heme protein catalytic carbene reaction and a lipase protein catalytic reaction are coupled to constructa green method for synthesizing quinoxaline compound by double proteins by a one-pot method, the target product can be rapidly and conveniently synthesized, meanwhile, synthesis is completed in water, and the problems that the existing quinoxaline compound preparation method comprises more synthesis steps and is not green and environmentally friendly enough are solved.

The invention discloses molecular imprinted magnetic nanoparticle capable of specifically adsorbing bovine hemoglobin, which belong to the technical field of biochemical separation. The molecular imprinted functionalized magnetic nanoparticles having the capability of specifically adsorbing a target protein can be prepared, the adsorption and separation conditions for bovine hemoglobin are mild bythe nanoparticles, the reaction is simple and rapid, and the specific adsorption of the target protein-bovine hemoglobin can be achieved in a protein mixed solution, which lays a good foundation forthe separation and purification of proteins.

The invention discloses a hemoglobin catalytic chemiluminescence enzyme-linked immunoassay method for diethylstilbestrol. The hemoglobin catalytic chemiluminescence enzyme-linked immunoassay method is characterized in that diethylstilbestrol is structurally modified to derive a hydroxyl group, and the hydroxyl group is connected with an amino group on a protein carrier, so that diethylstilbestrol is connected to the macromolecular protein carrier to form a complete antigen, then body immunoreaction is induced to synthesize a diethylstilbestrol artificial antigen, an antibody and a diethylstilbestrol enzyme-labeled antigen, and accordingly, ELISA can be performed. According to the method, the low-price hemoglobin (Hb) serves as a peroxidase mimic enzyme to replace a horse radish peroxidase, so that the Hb-labeled DES is prepared, and accordingly, the detection cost is greatly lowered.

The invention discloses a washing buffer solution. The washing buffer solution is composed of HEPES and a sulfhydryl compound, wherein the molar concentration of the HEPES in the buffer solution is 19-21 mM, the molar concentration of the sulfhydryl compound is 3-6 mM, and the pH value of the washing buffer solution is 7.5-7.8. The invention also discloses a method for purifying bovine hemoglobinby adopting the washing buffer solution. The washing buffer solution is convenient to prepare, can be used for purifying bovine hemoglobin by anion exchange chromatography, and can obtain high-puritybovine hemoglobin, and the purity can reach 99% or above. The method for purifying the bovine hemoglobin can simplify the anion exchange chromatography process, the purification method is simple, thehigh-purity bovine hemoglobin can be obtained by washing chromatography columns with the washing buffer solution, the yield can reach more than 90 percent, and the bovine hemoglobin can be directly used as a raw material for post-chemical cross-linking polymerization and has good industrial application prospect.

The invention provides a resuscitation fluid applied to the first aid of hemorrhagic shock of pets and a preparation method thereof, belongs to the field of a preparation method of resuscitation fluid, and solves problems that the conventional blood substitutes are single in component, only supply single oxygen and have fewer effects on the expanding of blood volume. The preparation method comprises the following steps: firstly, carrying out a reaction between bovine hemoglobin solution and glutaraldehyde solution, and centrifuging to obtain hemoglobin particles cross-linked with glutaraldehyde; and then, mixing the obtained hemoglobin particles cross-linked with glutaraldehyde with injection solution, to obtain the resuscitation fluid applied to the first aid of hemorrhagic shock of pets, wherein the injection solution is HES, HES2000 or physiological saline. The invention further provides the resuscitation fluid obtained according to the preparation method. The preparation method provided by the invention is simple; the raw materials are easy to obtain; the prepared resuscitation fluid can reversibly bind and release oxygen in vivo and simultaneously expand the blood volume.

The invention belongs to the field of analytical chemistry and quick detection, and particularly relates to a red-green-blue tri-fluorescent emission molecularly imprinted sensor based on a post-imprinting mixing method and a preparation method and application thereof in accurately and visually detecting hemoglobin. The red-green-blue tri-fluorescent emission molecularly imprinted sensor is obtained by mixing red fluorescent bovine hemoglobin imprinted microspheres, green fluorescent bovine hemoglobin imprinted microspheres and blue fluorescent bovine hemoglobin imprinted microspheres throughthe post-imprinting mixing method. The sensor prepared by the method can detect bovine hemoglobin in a high-sensitivity, high-selectivity and self-correcting manner, and the sensor is wider and richerthan conventional dual-fluorescent emission molecularly imprinted sensors in fluorescent color changing range and can visually detect a target more accurately.