Method for preparing bovine hemoglobin/titanium-based titanium dioxide hybrid material

A technology of bovine hemoglobin and titanium dioxide, which is applied in the field of preparation of bovine hemoglobin and titanium-based titanium dioxide hybrid materials, can solve the problems of non-continuous operation, inability to directly use powder performance test, and increased use cost to achieve low cost and good protein activity. , Easy device effect

Inactive Publication Date: 2012-06-20
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional TiO 2 Powder materials have a high specific surface area, but they cannot be operated continuously during application, and recycling and reuse will increase the cost of use
In addition, electrode materials cannot directly use powder for performance testing

Method used

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  • Method for preparing bovine hemoglobin/titanium-based titanium dioxide hybrid material
  • Method for preparing bovine hemoglobin/titanium-based titanium dioxide hybrid material
  • Method for preparing bovine hemoglobin/titanium-based titanium dioxide hybrid material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] a. Cut the purchased titanium sheet into a small rectangular piece of 1.0×2.0cm, and place it in isopropanol, acetone and methanol for 10 minutes for ultrasonic cleaning. Then ultrasonically cleaned with deionized water for 10 min at room temperature under N 2 Blow dry in the atmosphere;

[0030] b. Take 0.045mol NH 4 Dissolve F in a mixed solution of 1mL deionized water and 49mL ethylene glycol to prepare a 0.09mol / L electrolyte;

[0031] c. TiO by anodic oxidation 2Nano-array tube: connect the titanium and platinum electrodes to the anode and cathode of a DC stabilized power supply, apply a DC voltage of 40 volts for 6 hours, and keep the temperature of the electrolyte prepared in step b at 25°C throughout the process. After the electrolysis was completed, the sample was ultrasonically cleaned with deionized water for 30 seconds (carried out in 3 times), and the cleaned sample was washed with N 2 blow dry.

[0032] d.TiO 2 Calcination of nano-array tubes: put th...

Embodiment 2

[0038] a. Substrate treatment: Cut the purchased titanium sheet into small rectangular pieces of 1.0×2.0 cm, and place them in isopropanol, acetone and methanol for 10 minutes of ultrasonic cleaning. Then ultrasonically cleaned with deionized water for 10 min, under N 2 Blow dry in the atmosphere;

[0039] b. Preparation of electrolyte: take 0.01mol NH 4 Dissolve F in a mixed solution of 2mL deionized water and 98mL ethylene glycol to form an electrolyte;

[0040] c. TiO by anodic oxidation 2 Nano-array tube: connect the titanium and platinum electrodes to the anode and cathode of a DC stabilized power supply, apply a DC voltage of 20 volts for 2 hours, and keep the temperature of the electrolyte prepared in step b at 25°C throughout the process. After the electrolysis was completed, the sample was ultrasonically cleaned with deionized water for 30 seconds (carried out in 3 times), and the sample after cleaning was heated in N 2 Blow dry in the atmosphere;

[0041] d.TiO ...

Embodiment 3

[0046] a. Substrate treatment: Cut the purchased titanium sheet into 1.0×1.0cm rectangular pieces, and place them in isopropanol, acetone and methanol for 10 minutes of ultrasonic cleaning. Then ultrasonically cleaned with deionized water for 10 min, under N 2 Blow dry in the atmosphere;

[0047] b. Preparation of electrolyte: take 0.012mol NH 4 Dissolve F in a mixed solution of 2mL deionized water and 98mL ethylene glycol to form an electrolyte;

[0048] c. TiO by anodic oxidation 2 Nanotube array: connect titanium and platinum electrodes to the anode and cathode of a DC stabilized power supply, apply a DC voltage of 60 volts for 2 hours, and keep the temperature of the electrolyte prepared in step b at 25°C throughout the process. After the electrolysis was completed, the sample was ultrasonically cleaned with deionized water for 30 seconds (carried out in 3 times), and the sample after cleaning was heated in N 2 Blow dry in the atmosphere;

[0049] d.TiO 2 Calcination...

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Abstract

The invention relates to a method for preparing a bovine hemoglobin / titanium-based titanium dioxide hybrid material and belongs to the technical field of biological-inorganic hybridization. The method comprises the following steps of: preparing a titanium-based titanium dioxide nano array tubular film; and preparing a hemoglobin and titanium-based titanium dioxide nano array tube hybrid film. A highly ordered titanium dioxide nanotube array film is prepared by an anode oxidation method and the hemoglobin is fixed to the titanium-based titanium dioxide nanotube array film by using electrostatic self-assembly technology. The method has the advantages of simple operation and good enzyme activity keeping. The prepared hybrid film can be used in the fields of bio-sensing, biological electrochemical apparatuses and the like.

Description

technical field [0001] The invention belongs to the technical field of biological-inorganic hybridization, and in particular relates to a method for preparing a hybrid material of bovine hemoglobin and titanium-based titanium dioxide. The activity of protein in the hybrid material is kept good, and the catalytic oxidation activity to hydrogen peroxide is good. Background technique [0002] As an important branch of supramolecular chemistry, bio-inorganic hybrid technology has always been the focus of scientific research, and the construction and functionalization of bio-inorganic hybrid materials is a research hotspot recently and for a long time to come. Combining the characteristics of biomolecules with wide application potential, the functionalization and deviceization of biomacromolecules can be realized through bio-inorganic hybrid technology, so as to realize the value of biomolecules in practical applications. Active biomolecules (such as enzymes, amino acids, and pep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K17/14B01J31/38
Inventor 何静王艳
Owner BEIJING UNIV OF CHEM TECH
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