Hemoglobin catalytic chemiluminescence enzyme-linked immunoassay method for diethylstilbestrol

An immunological detection method and chemiluminescence enzyme technology, which are applied in the direction of chemiluminescence/bioluminescence, analysis by making materials react chemically, biological testing, etc., can solve problems that endanger the physical and mental health of consumers, and reduce detection costs and costs. Reduced effect

Inactive Publication Date: 2017-09-15
ZHENGZHOU UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, driven by strong economic interests, the phenomenon of illegal use of synthetic estrogen still exists, and many breeding users are still prohibited from using DES. The animals and animal food produced seriously endanger the physical and mental health of consumers.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hemoglobin catalytic chemiluminescence enzyme-linked immunoassay method for diethylstilbestrol
  • Hemoglobin catalytic chemiluminescence enzyme-linked immunoassay method for diethylstilbestrol
  • Hemoglobin catalytic chemiluminescence enzyme-linked immunoassay method for diethylstilbestrol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0048] The present invention will be described in detail below with reference to the drawings and specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. The test methods that do not indicate specific conditions in the following examples are usually operated according to conventional conditions, and the sources of unindicated experimental materials are commercially available. Since they do not involve the invention point, the steps are not described in detail.

[0049] When the examples give numerical ranges, it should be understood that, unless otherwise stated in the present invention, the two endpoints of each numerical range and any value between the two endpoints can be selected. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, equipment, and m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a hemoglobin catalytic chemiluminescence enzyme-linked immunoassay method for diethylstilbestrol. The hemoglobin catalytic chemiluminescence enzyme-linked immunoassay method is characterized in that diethylstilbestrol is structurally modified to derive a hydroxyl group, and the hydroxyl group is connected with an amino group on a protein carrier, so that diethylstilbestrol is connected to the macromolecular protein carrier to form a complete antigen, then body immunoreaction is induced to synthesize a diethylstilbestrol artificial antigen, an antibody and a diethylstilbestrol enzyme-labeled antigen, and accordingly, ELISA can be performed. According to the method, the low-price hemoglobin (Hb) serves as a peroxidase mimic enzyme to replace a horse radish peroxidase, so that the Hb-labeled DES is prepared, and accordingly, the detection cost is greatly lowered.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a hemoglobin-catalyzed chemiluminescent ELISA detection method for diethylstilbestrol. Background technique [0002] With the rapid development of modern society, people's material living standards have been significantly improved, and food safety problems have become more and more frequent. With the gradual development of modern animal husbandry in the direction of intensification and scale, farmers use veterinary drugs and feed additives in animal husbandry in order to reduce animal morbidity and mortality, improve feed utilization, promote animal growth, and shorten animal feeding cycles. It has been widely used in industrial production. However, excessive use of veterinary drugs can cause harm to the human body through the food chain, such as toxic reactions, drug-resistant strains, allergic reactions, intestinal flora imbalance, affecting the ecological environment ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/74G01N21/76
CPCG01N21/76G01N33/74
Inventor 王瑞勇尹瑜静
Owner ZHENGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap