50 results about "B cereus" patented technology

The present invention relates to a kind of streptokinase, and is especially one kind of Bacillus cereus produced fibrinolysin, or ceryl kinase. The ceryl kinase has HPLC fingerprint with peak within 7-9 min in the conditions of chromatographic column Waters Protein -PakTM 60 7.8*300 mm, mobile phase 0.2mol / L NaH2PO4-CH3OH(95:5), flow rate 1ml / min and detecting wavelength 220 nm. The present invention also provides the preparation process of the ceryl kinase, and the use of the Bacillus cereus and the ceryl kinase in preparing thrombus treating medicine. The ceryl kinase of the present invention, the rat carotid artery thrombosis experiment shows, possesses obvious thrombosis resisting effect, so that the present invention provides one new option for treatment of thrombus diseases.

The invention belongs to the field of feed, and in particular relates to a laying hen feed for producing high-calcium and high-zinc nutritious eggs and a preparation method thereof. The laying hen feed contains: base material fermented product, calcium lactate, zinc gluconate, glycyl glutamine and high nucleic acid yeast hydrolyzate; Bacillus and the product after ventilated fermentation; the inoculation amount of said Bacillus cereus is 0.05-0.8% by weight, the inoculum amount of Bacillus megaterium is 0.005-0.05% by weight; the content of calcium lactate is 8-15% by weight, glucose The content of zinc acid zinc is 0.1-0.5% by weight, the content of glycyl glutamine is 0.01-0.5% by weight, and the content of high nucleic acid yeast hydrolyzate is 0.1-0.5% by weight. The laying hen feed of the invention obviously improves the laying quality of laying hens, and reduces the amount of feed to be fed, thereby improving the production benefits of laying hen breeding.

The invention discloses a nucleic acid thermostatic microfluidic chip sensor with double aptamer function for microorganism detection. The sample solution, magnetic capture complex-dumbbell-shaped probe, RCA-luminescent system, and buffer solution are added dropwise to the microfluidic chip, and the separation, enrichment and Detection; the aptamer of Bacillus cereus is obtained through whole-cell screening and tailoring, and has high affinity and specificity. At the same time, the sensor has the advantages of complete functions, simple and portable, can complete the detection within 1 hour, and does not need to be expanded and cultivated.

The invention belongs to the field of the detection of microorganisms and discloses a method for rapidly and accurately detecting live waxy bacilli capable of producing vomitoxin and waxy bacilli incapable of producing vomitoxin simultaneously. A specific primer is designed aiming to (i)cesB( / i) of waxy bacilli encoded vomitoxin and (i)16SrRNA( / i) of waxy bacilli; the interference of dead waxy bacilli in foods is removed by using propidium monoazide (PMA); the DNA of live bacilli in foods is extracted through a boiling water bath method; polymerase chain reaction (PCR) detection is repeatedly carried out; a possible false negative result in a PCR system is indicated through the addition of non-competitive internal amplification control (IAC). By adopting the method, the interference of normal false positive and negative results in the detection through a molecular biological method is avoided and then detection result is more accurate; meanwhile, the detection time of the method is shorter than that of a traditional culture method.

The invention relates to the field of biotechnology, and concretely discloses a detection kit as well as a detection method and application for bacillus cereus. The detection kit is used for detecting the bacillus cereus through real-time fluorescence RPA, and comprises a pair of primers based on a bacillus cereus 16sRNA gene sequence and a probe. The detection method has excellent specificity and sensitivity, the detection limit is 1.0 x 10<-3>ng / muL, and when the pollution load is larger than or equal to 1.5 x 10<4>CFU / g, the bacillus cereus can be detected through the real-time fluorescence RPA, and only 6 to 13min is required. Therefore, the real-time fluorescence RPA established by the invention can be used for quickly, efficiently and sensitively detecting the bacillus cereus, and provides a referential technological means for detection of the bacillus cereus in food processing quality control, food-borne epidemic diseases and other public health events.