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50 results about "B cereus" patented technology
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Loop-mediated isothermal amplification (LAMP) primer pair of bacillus cereus and detection method
InactiveCN101831493AStrong specificityImprove featuresMicrobiological testing/measurementMicroorganism based processesLoop-mediated isothermal amplificationToxin
The invention relates to a primer pair and a method required by loop-mediated isothermal amplification (LAMP) detection of bacillus cereus causing vomit food poisoning. The primer pair can be used for preparing an LAMP detection kit which can be used for the rapid detection of the bacillus cereus expressing vomitoxin. The specific solution scheme comprises the step of designing a plurality of groups of LAMP inside and outside primer pairs including the whole target sequence by using a cesA / B gene with plasmid pCERP, carried by the vomit bacillus cereus as a target sequence. The invention comprises a vomit bacillus cereus cesA / B gene specificity LAMP primer pair, optimized reaction conditions, LAMP, minimum detection limit measurement and positive result identification. The LAMP method adopted by the vomit bacillus cereus has the advantages of high efficiency and rapid operation, high sensitivity and specificity, convenience, practicability and the like.
Owner:WUHAN POLYTECHNIC UNIVERSITY
Use of cereour bacillus in preparing thrombus treating medicine
InactiveCN1857722AProlong formation timeObvious antithrombotic effectBacteriaPeptide/protein ingredientsHplc fingerprintDisease
Owner:CHENGDU DIAO JIU HONG PHARMACEUTICAL FACTORY
Optimized saccharomyces cerevisiae strain capable of producing fucosyllactose at high yield and application of saccharomyces cerevisiae strain
The invention discloses an optimized saccharomyces cerevisiae engineering strain capable of highly producing 2'-fucosyllactose. Through modification by metabolic engineering, the saccharomyces cerevisiae can absorb lactose and takes GDP-mannose as a precursor in a cell to synthesize GDP-fucose, and alpha-1,2-fucosyltransferase derived from bacillus cereus is heterologously expressed. The 2'-fucosyllactose with a high yield can be obtained by the obtained recombinant strain, and the 2'-fucosyllactose yield can be increased to about 3.8 g / L through subsequent overexpression of the GDP-mannose synthesis related enzyme. Therefore, an important reference is provided for industrial production of the 2'-fucosyllactose from the saccharomyces cerevisiae.
Owner:SHANDONG UNIV
Saccharomyces cerevisiae engineering strain with high yield of fucosyllactose and application of saccharomyces cerevisiae engineering strain
The invention discloses a saccharomyces cerevisiae engineering strain with high yield of 2'-fucosyllactose. Through metabolic engineering modification, the saccharomyces cerevisiae can absorb lactoseand takes GDP-mannose as a precursor in a cell to synthesize GDP-fucose, alpha-1,2-fucosyltransferase derived from bacillus cereus is subjected to heterologous expression, the obtained recombinant strain can produce the 2'-fucosyllactose at a high yield, and an important reference is provided for industrial production of the 2'-fucosyllactose.
Owner:SHANDONG UNIV
Preparation method of chitosanase
InactiveCN108531418ALow costSimplify separation and purification stepsBacteriaMicroorganism based processesDEAE-SepharoseFreeze-drying
The invention discloses a preparation method of cihtosanase. Bacillid cereus 116 is used as starting strain, the extracellular chitosanase is prepared by optimizing a culture condition by virtue of liquid fermentation, the enzymatic activity of the chitosanase in the fermented solution can reach 40 to 80 U / mL, the fermented solution is frozen and centrifuged and precipitated level by level by virtue of ammonium sulfate, and the solid chitosanase is obtained by virtue of DEAE-Sepharose Fast Flow ion-exchange column chromatography, concentration and freeze drying.
Owner:INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
Substrate and method for improving survival rate of afforestation in drought and barren mountainous regions
ActiveCN104844311AImprove utilization efficiencyImprove survivalUrea compound fertilisersFertilizer mixturesImproved survivalBacillus cereus
The present invention discloses a substrate capable of improving the survival rate of afforestation in a drought and barren mountainous region. The substrate consists of a CLP-urea gel and a CLP-Z77 gel; the CLP-urea gel is prepared by uniformly mixing a urea solution and a CLP braking carrier in a proportion of (50-80)ml:1g, and the urea solution is absorbed by the CLP braking carrier, thus the CLP-urea gel is formed; the CLP-Z77 gel is prepared by uniformly mixing a bacillus cereus Z77 solution and the CLP braking carrier in a proportion of (15-30)ml:1g, and the bacillus cereus Z77 solution is absorbed by the CLP braking carrier, thus, the CLP-Z77 gel is formed. The present invention further discloses a method for afforestation in the drought and barren mountainous region by adopting the substrate. By adopting the substrate and the method, the survival rate and preserving rate of afforestation are remarkably improved.
Owner:SHANDONG FOREST SCI RES INST
Application and application method of bacillus cereus keratinase
ActiveCN112126536AImprove stabilityImprove heat resistanceHydrolasesMicroorganism based processesBiotechnologyMicrobiology
The invention belongs to the technical field of enzyme application, and particularly relates to application of bacillus cereus Y15 keratinase. The invention mainly protects the application of the bacillus cereus CereusY15 keratinase in the preparation of a descaling product, in particular to the application of the keratinase in the preparation of a washing product, and discloses the bacillus cereus CereusY15 keratinase. The keratinase disclosed by the invention is obtained by inoculating an optimized enzyme production culture medium with a B.cereus Y15 seed solution and culturing; the xylanasehas excellent thermal stability, and satisfies the following conditions: the enzyme activity half-life period at 40 DEG C is 34-36.5 h; the enzyme activity half-life period t1 / 2 at 60 DEG C is 5.5-6h, the half-life period at 65 DEG C is 2.2-2.5 h, and the half-life period at 70 DEG C is 0.58-0.65 h. In addition, the keratinase can tolerate a reducing agent, an oxidizing agent and a detergent, and has potential application value in industrial production.
Owner:BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
Method for fermenting liquid fertilizer by using beneficial microorganisms
InactiveCN113264793AEasy accessPromote decompositionBio-organic fraction processingBioloigcal waste fertilisersBiotechnologyNutrition
The invention relates to the technical field of microbial fermentation, and discloses a method for fermenting a liquid fertilizer by using beneficial microorganisms. The method comprises the following steps: A, weighing the following raw materials in parts by mass: 11-14 parts of traditional Chinese medicine residues, 120-320 parts of a microbial fermentation agent, 2-8 parts of a nutritional agent, 50-90 parts of water, 35-45 parts of a solvent, 1-4 parts of a stabilizer, 3-9 parts of an additive, 16-24 parts of soybean meal, 1.5-2.5 parts of amino acid, 5-10 parts of rice bran and 3-8 parts of molasses, B, pouring the soybean meal, the rice bran, the amino acid and the traditional Chinese medicine residues into a container, uniformly stirring, adding water, and controlling the water content to be 62-68%. The microbial fermentation agent and the nutritional agent are added into the raw materials, balanced nutrients are provided for plants or crops, the raw materials are easy to obtain, harmless to soil and easy to decompose, and the bacillus cereus, the bacillus subtilis, the lactobacillus, the lactic acid coccus, the streptococcus faecalis and the bifidobacterium can degrade heavy metals such as lead, cadmium, mercury and arsenic, so that the soil activity is effectively improved; and the crop yield is improved.
Owner:江苏绿科生物技术有限公司
Planting method of rice rich in selenium and SOD
InactiveCN110915581AReduce the number of timesSimple and fast operationRice cultivationBiotechnologyInsect pest
The invention belongs to the field of agricultural science and technology, and particularly discloses a planting method of rice rich in selenium and SOD. The planting method comprises the steps of treating seeds, raising seedlings, performing transplant rice seedlings, performing fertilizing, performing field management, performing controlling of diseases and insect pests and performing harvesting, wherein in the process of planting rice, a reinforcing agent is applied, and the reinforcing agent comprises bacillus cereus capable of generating SOD and selenium-containing fertilizers. Accordingto the planting method, the content of SOD and the content of trace element selenium in rice grains can be increased at the same time, and the method is simple and convenient.
Owner:张家界鱼泉生态农业开发有限公司
Application and Application Method of Bacillus Cereus Keratinase
ActiveCN112126536BImprove stabilityImprove heat resistanceHydrolasesMicroorganism based processesBiotechnologyMicrobiology
Owner:BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
Preparation for improving intestinal microbial environment and preparation method thereof
InactiveCN104116999AGood effectNo side effectsPowder deliveryBacteria material medical ingredientsBiotechnologyMedicinal herbs
The invention discloses a preparation for improving an intestinal microbial environment. The preparation is prepared from the following main raw materials in parts by weight: 3-9 parts of monkshood, 3-10 parts of jasmine, 9-18 parts of fortune eupatorium herb, 4-12 parts of massa medicata fermentata fujianensis, 3-10 parts of rhizoma nelumbinis, 5-12 parts of concha ostreae, 9-20 parts of notoginseng powder, 5-12 parts of fructus amomi and 0.3-1.0 part of bacillus cereus. The selected medicinal raw materials of the preparation are traditional Chinese medicinal materials, have no side effect, are easily available and low in cost and the preparation is prepared on the basis of research of a lot of published documents, pharmacological work and clinical tests, and has a good curative effect on diarrhea and constipation caused by a chaotic intestinal microbial environment.
Owner:王健芳
Application of bacillus cereus B-28 in aspect of repairing environment polluted by heavy metal Cr
InactiveCN112831323AIncrease biomassPromote growthPlant growth regulatorsBiocideBiotechnologyMicroorganism
The invention provides application of bacillus cereus B-28 in the aspect of repairing the environment polluted by heavy metal Cr. The content of ammonium nitrate extraction state Cr and DTPA extraction state Cr of plant rhizosphere can be remarkably reduced by inoculating a B-28 strain to a plant, the content of Cr in each tissue of the plant is remarkably reduced, the chlorophyll content in plant leaves is promoted to be increased, the activity of CAT and POD enzymes at the root is promoted to be enhanced, the biomass of each tissue of the plant is remarkably improved, and a new scheme for plant-microorganism combined passivation of Cr to promote plant growth is provided.
Owner:JINAN UNIVERSITY
Dual-aptamer functional nucleic acid constant-temperature micro-fluidic chip sensor for microbiological detection
ActiveCN114487401ASensitive Field DetectionEfficient on-site inspectionBiological material analysisLaboratory glasswaresAptamerMicroorganism
The invention discloses a dual-aptamer functional nucleic acid constant-temperature micro-fluidic chip sensor for microbiological detection. The method comprises the following steps: dropwise adding a sample solution, a magnetic capture complex-dumbbell-shaped probe, an RCA-luminescent system and a buffer solution onto a micro-fluidic chip, and realizing separation, enrichment and detection of bacillus cereus through control of a valve and an external magnetic field; wherein the aptamer of the bacillus cereus is obtained through whole-cell screening and cutting, and has high affinity and specificity. Meanwhile, the sensor has the advantages of complete functions, simplicity, portability, capability of completing detection within one hour, no need of enlarged culture and the like.
Owner:CHINA AGRI UNIV
Bacillus cereus NiR (nitrite reductase), gene and application
InactiveCN105063066AHigh purityAddressing Purity IssuesWater contaminantsEnzymesEscherichia coliNitrite reductase
The invention discloses a Bacillus cereus NiR (nitrite reductase), a gene and an application. The NiR gene in Bacillus cereus LJ01 is cloned and induced for expression in Escherichia coli with the PCR (polymerase chain reaction) technology, cells of blank-plasmid-containing BL21 subjected to IPTG (isopropyl beta-D-thiogalactopyranoside) induction, recombinant-plasmid-containing BL21 subjected to induction without IPTG and recombinant-plasmid-containing BL21 subjected to IPTG induction are subjected to ultrasonic crushing for extraction of crude enzymes, SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is performed on the crude enzymes for detection of recombinant protein expression, high-purity recombinant protein is obtained through affinity chromatography purification, and the problem about separation of NiR with higher purity and content from the Bacillus cereus is solved. A large amount of NiR recombinant protein can be used for degrading nitrite in food, non-acid environments and aquatic water.
Owner:SOUTH CHINA UNIV OF TECH
Bacillus cereus strain B2, liquid preparation, preparation method of liquid preparation and application in controlling ellinia trunk rot of hybridized bamboo
The invention discloses bacillus cereus strain B2, a liquid preparation, a preparation method of the liquid preparation and an application in controlling rosellinia necatrix of hybridized bamboo. The strain has been preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms (CGMCC), Chaoyang District, Beijing, China, since 20th June, 2013, and has a preservation number of CGMCC No.7767 and a classified name of bacillus cereus. The above liquid preparation is diluted by 100-200 times (volume ratio) and is poured to the root of each of non-pathogeny hybridized bamboos along root system breadth direction with 100 mL per plant and once in spring every year, thereby the generation of rosellinia necatrix can be prevented. The above liquid preparation is diluted by 50-100 times (volume ratio) and is poured to the root of each of morbidity hybridized bamboos along root system breadth direction with 200 mL per plant and once in spring and once in summer every year for three successive years, thereby rosellinia necatrix can be effectively cured.
Owner:SICHUAN AGRI UNIV
Harmless premix additive for pig
ActiveCN104000039AGrow fastReduced disease control costsFood processingAnimal feeding stuffBiotechnologyNutrition
The invention belongs to the field of culture techniques, and particularly relates to a harmless premix additive for pigs. The additive of the invention is prepared from certain amount of the following raw materials: calcium carbonate, calcium hydrogen phosphate, medical stone, compound enzyme, lysine, methionine, histidine, semen vaccariae, motherwort, semen raphani, anise, radix curcumae, cinnamon, tangerine peel and licorice; the premix additive also comprises 0.8-1.2 billions / kg of bacillus cereus, and the balance being wheat bran. The additive of the invention is a harmless premix additive for pigs, which is comprehensive in nutrition, low in mineral, free of heavy metal, free of hormone, free of clenbuterol, free of antibiotics, free of mildew inhibitors, free of preservatives, free of anti-oxidants, and free of arsenic or chromium preparations; the additive can accelerate live pig growth, reduce feed to gain ratio, enable pork to be appetizing, chewy, and free of fishy smell or peculiar smell, increase the protein content of pork, decrease the stearic acid content, and ensure the chewing moutfeel, the nutrition value and the edible safety of pork.
Owner:郭培星
Medicine for treating bowel dysfunction and preparation method thereof
InactiveCN104083633AEasy to get drug sourceLow costPowder deliveryDigestive systemMedicinal herbsBowel dysfunction
The invention discloses a medicine for treating bowel dysfunction, which is prepared from the following main raw materials in parts by weight: 4-12 parts of medicated leaven, 6-15 parts of morinda officinalis, 9-18 parts of eupatorium, 3-9 parts of monkshood, 3-9 parts of rheum officinale, 3-10 parts of root of common peony, 30-45 parts of rhizoma bletillae, 8-16 parts of grifola, and 0.3-1 part of bacillus cereus. The medicine uses the traditional Chinese medicines, and has no toxic and side effects, the raw materials are easy to obtain and have low cost, and the medicine has a good curative effect to bowel dysfunction.
Owner:王健芳
Viable bacteria mixture for treating diarrhea with flatulence and preparation method thereof
InactiveCN104116920ASignificant effectEasy to get drug sourcePowder deliveryBacteria material medical ingredientsBiotechnologyMedicinal herbs
The invention discloses a viable bacteria mixture for treating diarrhea with flatulence. The viable bacteria mixture is prepared from the following main raw materials in parts by weight: 3-9 parts of monkshood, 3-10 parts of jasmine, 4-12 parts of massa medicata fermentata fujianensis, 3-9 parts of saussurea lappa, 5-12 parts of poria with hostwood, 30-45 parts of rhizoma bletillae, 9-20 parts of chorion ovi and 0.3-1 part of bacillus cereus. The selected materials for the viable bacteria mixture are traditional Chinese medicinal materials; and the viable bacteria mixture is prepared from medicinal materials, is low in medicinal material cost, and has a good curative effect on the symptoms such as abdominal pain, fever, diarrhea, intestinal colic and flatulence.
Owner:王健芳
CAMP detection primer group of bacillus cereus enterotoxin gene and kit
ActiveCN110257541AQuick checkAccurate detectionMicrobiological testing/measurementAgainst vector-borne diseasesBacillus cereusBacillus aryabhattai
The invention relates to a CAMP detection primer group of a bacillus cereus enterotoxin gene and a kit. The primer group comprises two primers which are respectively a primer for amplifying the entFM region fragment of the bacillus cereus enterotoxin gene and a primer for amplifying the bceT region fragment of the bacillus cereus enterotoxin gene. The primer group provided by the invention is high in sensitivity and high in specificity, and the kit prepared by the primer group can quickly and accurately detect whether the sample contains bacillus cereus or not, and simultaneously judge whether the sample contains toxigenic genes entFM and bceT. Moreover, because the primer group provided by the invention has extremely high specificity, the time required for CAMP amplification is short, the detection time is further shortened, and operation is simplified.
Owner:SHENYANG AGRI UNIV
A kind of laying hen feed for producing high-calcium and high-zinc nutritious eggs and preparation method thereof
ActiveCN107259186BIncrease egg productionProlong laying cycleAnimal feeding stuffAccessory food factorsBiotechnologyBacillus megaterium
Owner:WUHAN POLYTECHNIC UNIVERSITY
A dual-aptamer functional nucleic acid thermostatic microfluidic chip sensor for microbial detection
ActiveCN114487401BSensitive Field DetectionEfficient on-site inspectionBiological material analysisLaboratory glasswaresAptamerMicroorganism
Owner:CHINA AGRI UNIV
Planting method of sod-rich cereal crops
ActiveCN110100668BIncreased SOD contentIncrease productionBiocidePlant growth regulatorsBiotechnologyBacilli
Owner:ZHENGZHOU INST OF AGRI & FORESTRY
Simultaneous rapid and accurate detection of live vomitoxin-producing and non-emetoxin-producing bacillus cereus kit and detection method in food
ActiveCN103725771BAvoid interferenceThe test result is accurateMicrobiological testing/measurementBiotechnologyPropidium monoazide
Owner:WUXI ZODOLABS BIOTECH
Multi-enzyme complex capable of efficiently degrading gluten protein and application of multi-enzyme complex
PendingCN114438044AEfficient degradationReduce security risksBacteriaPeptide/protein ingredientsEnzyme complexBacilli
The invention provides a multi-enzyme complex capable of efficiently degrading gluten protein. The multi-enzyme complex is a multi-enzyme complex derived from bacillus cereus AFA01. The multi-enzyme complex not only can efficiently degrade gluten protein, but also can efficiently degrade celiac disease causing peptides and destroy T cell epitopes in the celiac disease causing peptides. The enzyme preparation prepared from the multi-enzyme complex can be used as an oral enzyme preparation for assisting human body in digesting gluten protein and is used for assisting in treating celiac disease; the strain can also be used in food processing, can more effectively degrade gluten protein, and is expected to provide safe and reliable hypoallergenic food for celiac disease patients.
Owner:NANCHANG UNIV
Detection kit, detection method and application for bacillus cereus
ActiveCN107236812BSimple and fast operationShort reaction timeMicrobiological testing/measurementBiotechnologyPublic health
Owner:INSPECTION & QUARANTINE TESTING CENT OF HEBEI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Bacillus cereus high-density fermentation method and application
The invention discloses a high-density fermentation method of bacillus cereus and application, and belongs to the technical field of fermentation engineering. According to the high-density fermentation method, high-density fermentation of the bacillus cereus is carried out through a strategy of regularly and instantaneously supplementing nutrient substances required by thallus growth, converting different alkali liquor at a thallus growth stage to maintain pH in combination with a three-stage dissolved oxygen regulation and control mode. After fermentation is finished, the dry weight of the bacillus cereus reaches 40-48 g / L and is increased by 70-135% in comparison with traditional continuous fermentation, and is increased by 328% in comparison with a non-fed-batch fermentation method. The method provided by the invention effectively increases the dry weight of thalli of the bacillus cereus, is simple and easy to implement, and has a wide application prospect.
Owner:JIANGNAN UNIV +1
Bacillus cereus immune colloidal gold rapid detection test strip and preparation method thereof
The invention discloses a bacillus cereus immune colloidal gold rapid detection test strip. The test strip comprises a PVC bottom plate, a sample pad arranged on the PVC bottom plate, a combination pad adjacent to the sample pad and a water absorption pad adjacent to the combination pad, and a nitrocellulose membrane layer is arranged between the conjugate pad and the PVC bottom plate, and a detection line and a quality control line are arranged on the conjugate pad. According to the invention, the method can be used for detecting the immunogen of the bacillus cereus, and the detection sensitivity is high.
Owner:UNIV OF SHANGHAI FOR SCI & TECH
A kind of preparation method of neutral endo-pectate lyase
ActiveCN107937378BEasy to separateTo achieve the purpose of degummingBacteriaMicroorganism based processesBiotechnologyUltrafiltration
The invention discloses a preparation method of neutral endo-pectate lyase. The preparation method comprises the following steps: inoculating cultured mature bacillus cereus WENA703 into a seed tank from an inclined plane of an eggplant bottle, and performing secondary fermentation in a fermentation tank to produce fermented wort; and performing tubular centrifugation, plate frame filtration, ultrafiltration concentration, paperboard diatomite sterilization and standardization on the fermented wort to prepare the neutral pectate lyase with the enzyme activity being more than or equal to 300000u / ml, and performing anti-corrosion treatment to obtain standard high-activity neutral pectate lyase liquid keeping the enzyme activity to be 90% or more (at 25 DEG C for six months), wherein the bacillus cereus WENA703 is preserved with a preservation number of CCTCC NO:M2017368 in the China Center for Type Culture Collection. By adopting the technical scheme of the invention, the produced neutral pectate lyase system can better adapt to a paper-making process of waste paper.
Owner:南宁雄晋生物科技有限公司
A liquid chip for detecting Bacillus cereus and its application
ActiveCN112575097BShort detection timeHigh detection throughputMicrobiological testing/measurementMicroorganism based processesBiotechnologyMicrosphere
Owner:NANKAI UNIV
High-yield bacillus cereus industrial production and fermentation process
PendingCN112410238AReduce the cost of industrial-grade fermentationReduce fermentation costsBacteriaMicroorganism based processesBiotechnologyDipotassium phosphate
The invention discloses a high-yield bacillus cereus industrial production and fermentation process. The problems of low yield and high cost in the bacillus cereus processing process are solved. According to the key point of the technical scheme, the high-yield bacillus cereus industrial production and fermentation process comprises the steps of primary culture, secondary culture, tertiary culture, fermentation tank transfer and drying treatment, and is applied to a 5000L seed tank and a 60m<3> fermentation tank. Raw materials of a seed culture medium and a fermentation culture medium are thesame, and the seed culture medium and the fermentation culture medium are each prepared from the raw materials: bean pulp meal, soybean meal, corn flour, glucose, a bran leachate, sodium chloride, ammonium sulfate, dipotassium phosphate, magnesium sulfate and calcium chloride. The purposes of increasing the product yield and reducing the production cost are achieved.
Owner:朝阳华星生物工程有限公司
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