High-yield bacillus cereus industrial production and fermentation process

A Bacillus cereus, fermentation process technology, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of high cost, low product quality, inability to produce high yields, etc., to reduce the fermentation cost, increase the number of viable fermentation bacteria, and increase the profit value. Effect

Pending Publication Date: 2021-02-26
朝阳华星生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the production process of the existing Bacillus cereus, due to the high cost of carbon source and nitrogen source in the fermentation step, mass production fermentation cannot be realized, and the number of viable fermentation bacteria in the existing fermentation technology is low, resulting in product low quality and incapable of high yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A high-yielding Bacillus cereus industrial production fermentation process, comprising: 1) primary cultivation: inoculate the Bacillus into an eggplant bottle containing a culture solution for activation treatment, cultivate it in a culture shaker to obtain primary seeds, and place the primary seeds Inoculate into a 50L seed tank, adjust the pH value to 6.4, and cultivate at 36°C for 15 hours in an aerobic environment, and put into the seed medium to form first-class seeds;

[0024] 2) Secondary cultivation: Inoculate primary seeds into a 300L seed tank, adjust the pH value to 6.4, and cultivate at 36°C for 18 hours in an aerobic environment, and put into the seed medium to form secondary seeds;

[0025] 3) Tertiary cultivation: sterilize the 5000L seed tank at a temperature of 121°C for 30 minutes, inoculate the secondary seeds into the sterilized 5000L seed tank, control the batching volume to 3000L, and adjust the 5000L seed tank After the sterilization, the pH value...

Embodiment 2

[0030] A high-yielding Bacillus cereus industrial production fermentation process, comprising: 1) primary cultivation: inoculate the Bacillus into an eggplant bottle containing a culture solution for activation treatment, cultivate it in a culture shaker to obtain primary seeds, and place the primary seeds Inoculate into a 50L seed tank, adjust the pH value to 6.7, and cultivate at a temperature of 37°C for 18 hours in an aerobic environment, and put into the seed medium to form first-class seeds;

[0031] 2) Secondary cultivation: Inoculate primary seeds into a 300L seed tank, adjust the pH value to 6.7, and cultivate at 37°C for 21 hours in an aerobic environment, and put into the seed medium to form secondary seeds;

[0032] 3) Tertiary cultivation: sterilize the 5000L seed tank at a temperature of 124°C for 30 minutes, inoculate the secondary seeds into the sterilized 5000L seed tank, control the batching volume to 3000L, and adjust the actual temperature of the 5000L seed ...

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PUM

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Abstract

The invention discloses a high-yield bacillus cereus industrial production and fermentation process. The problems of low yield and high cost in the bacillus cereus processing process are solved. According to the key point of the technical scheme, the high-yield bacillus cereus industrial production and fermentation process comprises the steps of primary culture, secondary culture, tertiary culture, fermentation tank transfer and drying treatment, and is applied to a 5000L seed tank and a 60m<3> fermentation tank. Raw materials of a seed culture medium and a fermentation culture medium are thesame, and the seed culture medium and the fermentation culture medium are each prepared from the raw materials: bean pulp meal, soybean meal, corn flour, glucose, a bran leachate, sodium chloride, ammonium sulfate, dipotassium phosphate, magnesium sulfate and calcium chloride. The purposes of increasing the product yield and reducing the production cost are achieved.

Description

technical field [0001] The invention belongs to the technical field of bacillus processing, in particular to a high-yielding bacillus cereus industrial production fermentation process. Background technique [0002] Bacillus cereus is a commonly used probiotic. After Bacillus cereus enters the intestinal tract, it consumes excess oxygen in the intestinal tract, creates an anaerobic environment, promotes the growth of anaerobic bacteria, adjusts the imbalance of intestinal flora, improves the micro-ecological environment, and inhibits the growth of harmful aerobic bacteria. It is beneficial to the survival of beneficial anaerobic bacteria, improves the feed utilization rate of animals and promotes the growth of animals. Pruss research shows that Bacillus cereus can also synthesize plasmin like other Bacillus. [0003] Adding Bacillus cereus to the feed can improve the feed conversion rate of weaned piglets and reduce the post-weaning diarrhea rate of piglets. Studies on mic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N3/00C12R1/085
CPCC12N1/20C12N3/00
Inventor 韩国宝梁爱民张天航孟欣达
Owner 朝阳华星生物工程有限公司
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