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A kind of preparation method of neutral endo-pectate lyase

A technology of pectate lyase and endo-cut fruit, which is applied in the biological field, can solve problems such as inability to adapt, and achieve the effects of improved spinnability, uniform fiber strength, and reduced production costs

Active Publication Date: 2021-03-19
南宁雄晋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still many problems in its wide application, resulting in the reality of selective use by enterprises
[0005] In addition, the enzyme production activity of the strains in the prior art is generally not well adapted to the papermaking process of waste paper at the same time.

Method used

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  • A kind of preparation method of neutral endo-pectate lyase
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  • A kind of preparation method of neutral endo-pectate lyase

Examples

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Effect test

preparation example Construction

[0071] The preparation method of the composite enzyme preparation of the present invention is as follows: the raw material enzyme preparations are subjected to pretreatment respectively, and then mixed uniformly in proportion, then an appropriate amount of preservative and enzyme protective agent are added, sterilized by a diatomaceous earth filter, and then passed through the determination Enzyme activity, quantitative packaging, and become a product.

[0072] The invention also discloses a waste paper papermaking process, in which the compound enzyme preparation is continuously added through a metering pump into a hydraulic pulper in the waste paper pulping process, and the compound enzyme preparation is added according to the standard of 100 grams per ton of dry raw material.

[0073] The components and mass percentages of the compound enzyme preparation are as follows:

[0074] Neutral endo-pectate lyase (enzyme activity ≥ 20000u / ml): 25%-35%;

[0075] Endo-cellulase (enz...

Embodiment 1

[0089] On July 11, 2017, Dongguan Xufeng Paper Co., Ltd. began to try out the compound enzyme preparation of the present invention. It was added and used at 11:00 am on July 11, and the trial ended on September 21. After more than 60 days of application Test, the specific test data are shown in the table below:

[0090] Table 1 Test data

[0091]

[0092]

[0093] Among them: due to the failure of the retention aid adding pump during September 12-September 16, the data during the period were not included in the statistics.

[0094] The test data in Table 1 above shows that:

[0095] 1. The concentration of the Internet is reduced, and the load of the network department is reduced;

[0096] 2. The concentration of white water under the screen is reduced, and the retention rate is significantly improved, which is beneficial to sludge reuse;

[0097] 3. The water line is shortened, and the filterability is enhanced, which is beneficial to save steam.

[0098] Table 2 Co...

Embodiment 2

[0112] On April 28, 2017, Guangzhou Huadu Changxing Paper Co., Ltd. began to try out a series of papermaking enzyme products provided by the present invention ---, as of June 2, after 35 days of continuous application tests, the specific test data are as follows:

[0113] Table 4 Test data comparison

[0114]

[0115] Table 5: Analysis of economic benefits per ton of paper after using papermaking enzymes (estimation)

[0116]

[0117] Note: Papermaking enzymes are calculated at 80,000 yuan / ton (excluding tax).

[0118] From the above data analysis, it can be known that the composite enzyme preparation of the present invention can achieve the following effects:

[0119] 1. The sticky matter is significantly reduced, and the sticky matter in the drying section is significantly reduced, and the paper breakage caused by this is reduced;

[0120] 2. The speed difference of the wire section is significantly reduced, the dehydration of the wire section is obviously improved, th...

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Abstract

The invention discloses a preparation method of neutral endo-pectate lyase. The preparation method comprises the following steps: inoculating cultured mature bacillus cereus WENA703 into a seed tank from an inclined plane of an eggplant bottle, and performing secondary fermentation in a fermentation tank to produce fermented wort; and performing tubular centrifugation, plate frame filtration, ultrafiltration concentration, paperboard diatomite sterilization and standardization on the fermented wort to prepare the neutral pectate lyase with the enzyme activity being more than or equal to 300000u / ml, and performing anti-corrosion treatment to obtain standard high-activity neutral pectate lyase liquid keeping the enzyme activity to be 90% or more (at 25 DEG C for six months), wherein the bacillus cereus WENA703 is preserved with a preservation number of CCTCC NO:M2017368 in the China Center for Type Culture Collection. By adopting the technical scheme of the invention, the produced neutral pectate lyase system can better adapt to a paper-making process of waste paper.

Description

[0001] The present invention relates to the preservation of biological material samples. The classification name of the biological material is: Bacillus cereus (Bacillus cereus) WENA703, which has been preserved in the China Type Culture Collection Center on June 23, 2017, and the address is China.Wuhan.Wuhan University, the deposit number is CCTCC NO: M2017368. technical field [0002] The invention belongs to the field of biotechnology, in particular to a preparation method of neutral endo-pectate lyase. . Background technique [0003] Although waste paper is a renewable resource that can be used to avoid the environmental pollution caused by pulping and alkali recovery, when producing waste paper pulp regardless of the use of natural waste paper and cardboard or white waste paper, it contains various wastes. Impurities will also be discharged into waste water to pollute the environment. The pulping system configured by large-scale waste paper and papermaking enterprises...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/88C12N9/42C12N9/24C12N9/20C12N9/02C12N9/96C12N1/20D21C5/00C12R1/085
CPCC12N1/20C12N9/0061C12N9/20C12N9/2437C12N9/2485C12N9/88C12N9/96C12Y110/03002C12Y301/01003C12Y302/01004C12Y302/01032C12Y402/02002D21C5/005
Inventor 庄冉郝志军周广军
Owner 南宁雄晋生物科技有限公司
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