31 results about "Artemisia aucheri" patented technology

The invention relates to a method for using the effective part of sweet wormwood polysaccharide to prepare drug. Wherein, said effective part is extracted from sweet wormwood, whose polysaccharide content is higher than 50% at 5000-50000 molecule weight, as well asisodulcitol, arabinose, xylose, mannose, cerebrose and amylaceum. The invention can be used to prepare the anti-cancer drug or immunity drug.

The invention relates to the field of biological technologies and provides a method for increasing content of artemisinin in Artemisia annua L. by genetic modification with CHI (chalcone Isomerase) genes. According to the method, the CHI genes are cloned from Artemisia annua L., a plant expression carrier containing the CHI genes is established, the CHI genes are mediated by agrobacterium tumefaciens to be transferred into Artemisia annua L. for regeneration of a plant, the integration condition of an exogenous target gene CHI is detected through the PCR (polymerase chain reaction), the content of artemisinin in transgenic Artemisia annua L. is detected through an HPLC-ELSD (high performance liquid chromatography-evaporative light scattering detector), and the transgenic Artemisia annua L. plant with the content of artemisinin increased is screened out. The content of artemisinin in transgenic Artemisia annua L. is increased and is 1.5 times that of a non-transgenic contrast plant in the highest level, and the method for increasing the content of artemisinin in Artemisia annua L. is provided and lays a foundation for large-scale production of artemisinin from transgenic Artemisia annua L..

The invention discloses a fast propagation method of tissue culture of traditional Chinese medicine Artemisia annua. The fast propagation method of tissue culture of traditional Chinese medicine Artemisia annua is that culture a terminal bud or an axillary bud of Artemisia annua are cultured in an initial medium so as to obtain an aseptic seedling. The initial medium is a medium of MS medium added with 6-BA and GA. The final concentration of the 6-BA is 0.1 to 2.0 mg / l and the GA is 0.2 to 2.0mg / l. The invented method has the advantages of easy avail of materials and aseptic materials, low variation rate, high enhancement factor, 5 to 6 times of multiple in 4 weeks, over 93 percent of rooting rate of tissue culture seedlings, over 95 percent of survival rate of transplanting and very strong commercialized production capacity. The high productive Chinese medicine Artemisia annua is reproduced by making use of the invention, and the high arteannuin of the offspring can be stabilized, thus increasing the output of arteannuin.

The invention discloses Artemisia annua L. PDR sub-family transport protein. Amino acid sequences of the protein comprise the amino acid sequence shown as SEQ ID NO:2 or the amino acid sequence of the protein is shown as SEQ ID NO:2 or nucleic acid codes crossed by the protein and complementary chains of nucleic acid of protein with the coded amino acid sequence shown in SEQ ID NO:2 under the highly-strict condition are achieved. Furthermore, the protein is named AaPDR1 by the inventor and is transport protein specifically expressed by Artemisia annua L. secreting type gladular trichomes, and it is proved through a yeast transport experiment and interference of Artemisia annua L. in the transport protein that the AaPDR1 participates in transporting an artemisinin synthetic route intermediate product dihydroartemisinic acid in Artemisia annua L.. The Artemisia annua L. PDR sub-family transport protein has great significance in providing high-yield and stable new drug resources for large-scale production of artemisinin.

The invention relates to a detecting method for a traditional Chinese medicinal preparation for treating cold. The detecting method comprises the steps of identifying characters, checking, identifying components and determining the content, wherein the component identifying step comprises the following specific steps: identifying grape-leaved anemone roots, identifying radix scutellariae, identifying radix glycyrrhizae, identifying sun-dried radix ginseng, identifying herba artemisiae scopariae and identifying herba artemisiae apiaceae; the content identifying step means content determination of baicalin. Aiming at a current detecting method that more comprehensive monitoring of effective components of the traditional Chinese medicinal preparation cannot be realized, and interfusion of the grape-leaved anemone roots as a counterfeit drug and the herba artemisiae scopariae as a confused material cannot be monitored, the invention formulates a component identifying and content determining method with scientificity, reasonability and feasibility; by using the component identifying and content determining method, clinical curative effect of a medicine is ensured, several medicinal materials are enabled to have specific quality indexes, and use of all the medicinal materials in the medicine is ensured, thereby ensuring the curative effect of the medicine; besides, the grape-leaved anemone roots as the counterfeit drug of radix bupleuri and the herba artemisiae scopariae as the confused material of the herba artemisiae apiaceae can be effectively detected, and further the harm to the health of patients caused by toxic and harmful ingredients is avoided.

The invention discloses a method for treating and increasing arteannuin content in sweet wormwood through carbonyl chloride (CoC12). The method is characterized in that seeds of sweet wormwood are planted into a seedling sprouting culture medium for sprouting, and then sweet wormwood seedlings are transplanted into a peat perlite culture medium and are irrigated with a common nutrient solution once per week, in the earlier stage of flowering, a CoC12 solution is used for treating the sweet wormwood, and then the sweet wormwood is collected on schedule. The method can effectively improve the arteannuin content in the sweet wormwood, is simple in operation, and free of higher earlier stage investment demanded by a genetic engineering method, effectively lowers production cost of arteannuin, and has significance for meeting arteannuin medical industry mass production.

The invention relates to a method of utilizing the pts gene and antisense ads to increase patchouli alcohol content in Artemisia annua L. plants. Using transgenic Artemisia annua L. plants, the method of the invention consistently increases the patchouli alcohol content in those plants, thus laying down a solid foundation for large-scale production of patchouli alcohol and other secondary metabolites such as terpenes other than artemisinin.

The invention relates to a hybridization breeding method of a traditional Chinese medicinal material-sweet wormwood and belongs to the technical field of genetic breeding of plants. The hybridization breeding method of the traditional Chinese medicinal material-sweet wormwood comprises the following steps: collecting different sweet wormwood sources and selecting a single plant with the high yield of sweet wormwood leaves and the high content of artemisinin; carrying out insulation and autocopuation by using a breeding bag after the pregnancy of buds and in ahead of the blooming stage, and continuously carrying out autocopuation for more than three generations; carrying out single plant tissue culture by starting from the fourth generation and carrying out hybridization combining ability tests to select male parents and female parents; and finally carrying out expanding propagation on the selected single plants obtained from tissue culture, inserting and planting the male parents and the female parents in an isolation area, carrying out natural pollination and respectively collecting seeds of the male parents and the female parents. The hybridization breeding method of the traditional Chinese medicinal material-sweet wormwood is capable of culturing different generations of sweet wormwood autocopuation systems, culturing and producing sweet wormwood hybrid seeds with the stable yield of the sweet wormwood leaves and the high content of the artemisinin; the method is simple to operate, mild in conditions and suitable for agricultural industrialization promotion.