A kind of hybrid breeding method of Chinese medicinal material Artemisia annua

Abstract

The invention relates to a hybridization breeding method of a traditional Chinese medicinal material-sweet wormwood and belongs to the technical field of genetic breeding of plants. The hybridization breeding method of the traditional Chinese medicinal material-sweet wormwood comprises the following steps: collecting different sweet wormwood sources and selecting a single plant with the high yield of sweet wormwood leaves and the high content of artemisinin; carrying out insulation and autocopuation by using a breeding bag after the pregnancy of buds and in ahead of the blooming stage, and continuously carrying out autocopuation for more than three generations; carrying out single plant tissue culture by starting from the fourth generation and carrying out hybridization combining ability tests to select male parents and female parents; and finally carrying out expanding propagation on the selected single plants obtained from tissue culture, inserting and planting the male parents and the female parents in an isolation area, carrying out natural pollination and respectively collecting seeds of the male parents and the female parents. The hybridization breeding method of the traditional Chinese medicinal material-sweet wormwood is capable of culturing different generations of sweet wormwood autocopuation systems, culturing and producing sweet wormwood hybrid seeds with the stable yield of the sweet wormwood leaves and the high content of the artemisinin; the method is simple to operate, mild in conditions and suitable for agricultural industrialization promotion.

Description

technical field [0001] The invention relates to a method for hybrid breeding of Chinese medicinal materials, in particular to a method for hybrid breeding of Artemisia annua which can increase the yield per mu of dried leaves of Artemisia annua and the content of artemisinin, and belongs to the technical field of plant genetics and breeding. Background technique [0002] Artemisia annua (Artemisia annua L.), also known as Artemisia annua, is an annual herb of the genus Artemisia in the family Asteraceae. Artemisia annua is mainly distributed in Jilin, Liaoning, Hebei, Shaanxi, Shandong, Hubei, Hunan, Sichuan, Guangdong, Guangxi, Guizhou and other provinces and regions in my country. It is a traditional Chinese medicine in China. Mainly above ground. In the 1970s, Chinese scientists discovered artemisinin in Artemisia annua, and confirmed that artemisinin has a significant effect in treating malaria. The medicinal part of Artemisia annua used as the raw material for extractin...

AI Technical Summary

Problems solved by technology

[0004] Due to the rapid expansion of the artemisinin industry, the wild Artemisia annua in the main production areas has been over-harvested for many years, the distribution of wild Artemisia annua communities has decreased sharply, the competition among artemisinin production enterprises is fierce, and the demand for artemisinin drugs in the international market is gradually increasing , the quantity and low content of wild Artemisia annua leaves can no longer meet the needs of artemisinin extraction. At present, the raw materials of Artemisia annua leaves are mainly planted by farmers, with a total annual planting scale of 100,000 to 200,000 mu. Generally 7‰~10‰

[0005] At present, it is generally believed that because Artemisia annua is a cross-pollinated plant and has "self-incompatibility", it is impossible to stabilize the traits of excellent lines through selfing to form inbred lines

Artemisia annua breeding units all use systematic breeding methods to cultivate conventional varieties / lines, but conventional varieties / lines are difficult to industrialize, and breeding research is difficult to obtain corresponding economic and sustainable support, resulting in the widespread use of Artemisia annua planting varieties / lines in major production areas. Homogenization, artemisinin content and yield stagnated

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